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丹栀逍遥散调控Beclin-1相关自噬诱导乳腺癌细胞铁死亡的机制研究
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作者 李慧 刘立萍 +2 位作者 杨鹤丹 陆飞 李然 《时珍国医国药》 北大核心 2025年第14期2622-2629,共8页
目的研究丹栀逍遥散含药血清对乳腺癌MDA-MB-231细胞铁死亡的调控作用及机制。方法将MDA-MB-231细胞分为空白对照(NC)、空白血清(Ctrl)、丹栀逍遥散(DZXY)、铁死亡诱导剂(SAS)、铁死亡抑制剂(Fer-1)以及Fer-1+DZXY组,用噻唑蓝比色法(MTT... 目的研究丹栀逍遥散含药血清对乳腺癌MDA-MB-231细胞铁死亡的调控作用及机制。方法将MDA-MB-231细胞分为空白对照(NC)、空白血清(Ctrl)、丹栀逍遥散(DZXY)、铁死亡诱导剂(SAS)、铁死亡抑制剂(Fer-1)以及Fer-1+DZXY组,用噻唑蓝比色法(MTT)和Annexin V/PI双染色法分别检测各组的增殖率及死亡情况,透射电镜观察线粒体形态,试剂盒检测丙二醛(MDA)、超氧化物歧化酶(SOD)、亚铁离子(Fe^(2+))、谷胱甘肽(GSH)的含量,蛋白免疫印迹法(Western blot)检测谷胱甘肽过氧化物酶4(GPX4)、铁蛋白重链亚基(FTH1)、转铁蛋白受体(TFR1)、铁转运蛋白(FPN1)、铁死亡抑制蛋白1(FSP1)、BECN1基因(Beclin-1)及微管相关蛋白1轻链3(LC3)的表达。结果与Ctrl组比较,DZXY组细胞的增殖率下降(P<0.01),死亡数量增多(P<0.01),MDA、Fe^(2+)含量上升(P<0.01、P<0.05),SOD、GSH水平下降(P<0.01、P<0.05),TFR1、Beclin-1、LC3-II/I的蛋白表达升高(P<0.01、P<0.05),GPX4、FTH1、FPN1、FSP1的蛋白表达降低(P<0.01,P<0.05);Fer-1组各项指标的变化呈相反趋势,而Fer-1+DZXY联合可以一定程度上逆转这些改变。此外,透射电子显微镜观察到药物干预后细胞出现线粒体皱缩等形态变化。结论丹栀逍遥散含药血清可能通过调控Bec⁃lin-1自噬促进MDA-MB-231细胞的铁死亡。 展开更多
关键词 乳腺癌 丹栀逍遥散 铁死亡 自噬 铁代谢
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Key fungal communities related to alleviating replanting stress of Lanzhou lily under silicon fertilizer and microbial agents application
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作者 WANG Yi-qin YU Yan-lin +5 位作者 YANG Hong-yu li hui HOU Lei MAN Hua-li HAN Jia SHI Gui-ying 《植物营养与肥料学报》 北大核心 2025年第2期395-406,共12页
【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference fo... 【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor. 展开更多
关键词 Lanzhou lily soil fungi diversity pathotroph saprophyte silicon fertilization microbial agent
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Regulatory effects of seed soaking with a beneficial microbial agent on the rhizosphere soil microflora of edible lilies
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作者 MAN Hua-li li hui +3 位作者 SHI Gui-hong YANG Hong-yu li Mou-qiang SHI Gui-ying 《植物营养与肥料学报》 北大核心 2025年第5期994-1005,共12页
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ... 【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health. 展开更多
关键词 edible lily second-generation sequencing technology(NGS) seed treating fungal community structure bacterial community structure PROPHYLAXIS
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Strength Development of Alkali-activated Binders Prepared with Mechanically Ground Fly Ash During Microwave-curing
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作者 ZHU huimei liU Yu li hui 《材料导报》 北大核心 2025年第20期108-114,共7页
Microwave-curing and mechanical grinding of fly ash have both beenadopted as effective methods for improving the early-age strength of alkali-activated fly ash(AAFA)binders.This study combined these two approaches by ... Microwave-curing and mechanical grinding of fly ash have both beenadopted as effective methods for improving the early-age strength of alkali-activated fly ash(AAFA)binders.This study combined these two approaches by synthesizing AAFA using original,medium-fine,and ultrafine fly ash as precursors,and then specimens were cured with a five-stage temperature-controlled microwave.The compressive strength results indicate that the original AAFA develops the highest strength initially during microwave-curing,reaching 28 MPa at stage 2.Medium-fine AAFA exhibits the highest strength of 60 MPa when cured to stage 4-I,which is 26%higher than the peak strength of original AAFA.It is attributed to the significant rise in their specific surface area,which accelerates the dissolution of Si and Al from the precursor and facilitates the subsequent formation of N-A-S-H gels.Additionally,nanoscale zeolite crystals formed as secondary products fill the tiny gaps between amorphous products,thereby significantly improving their microstructure.In contrast,ultrafine fly ash,primarily composed of fragmented particles,necessitated a substantial amount of water,which adversely affects the absorption efficiency for microwave of AAFA specimens.Thus,ultrafine AAFA specimens consistently exhibit the lowest compressive strength.Specifically,at the end of curing,the compressive strength of these three specimens with microwave-curing is approximately 32%,59%,and 172%higher than that of the steam-cured sample,respectively.These findings demonstrate the compatibility of microwave-curing and fly ash refinement in enhancing the early compressive strength development of AAFA. 展开更多
关键词 alkali-activated fly ash binder microwave-curing particle size compressive strength
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Cyclic behavior of root-loess composites under direct simple shear test conditions and insights from discrete element method modeling
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作者 SUN Yuan li hui CHENG Zhifeng 《水利水电技术(中英文)》 北大核心 2025年第S1期665-680,共16页
Plant roots are widely known to provide mechanical reinforcement to soils against shearing and further increase slope stability.However,whether roots provide reinforcement to loess cyclic re-sistance and how various f... Plant roots are widely known to provide mechanical reinforcement to soils against shearing and further increase slope stability.However,whether roots provide reinforcement to loess cyclic re-sistance and how various factors affect roots reinforcement during seismic loading have rarely been studied.The objective is to conduct a series of cyclic direct simple shear tests and DEM numerical simulation to investigate the cyclic behaviour of rooted loess.The effects of initial static shear stress and loading frequency on the cyclic resistance of root-soil composites were first investigated.After that,cyclic direct simple shear simulations at constant volume were carried out based on the discrete element method(PFC^(3D))to investigate the effects of root geome-try,mechanical traits and root-soil bond strength on the cyclic strength of rooted loess.It was discovered that the roots could effectively improve the cyclic resistance of loess.The cyclic resistance of the root-soil composite decreases with the increase of the initial shear stress,then increases,and improves with the increase of the frequency.The simulation result show that increases in root elastic modulus and root-soil interfacial bond strength can all enhance the cyclic resistance of root-soil composites,and the maximum cyclic resistance of the root-soil composite was obtained when the initial inclination angle of the root system was 90°. 展开更多
关键词 root-soil composite cyclic direct simple shear tests PFC^(3D)
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Key Technologies for Security Control of 5G-R Terminal and Application Access
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作者 MA Jun liU Chang +2 位作者 liN Wei li hui HAN Xu(Translated) 《Chinese Railways》 2025年第1期3-10,共8页
Terminals and their access represent a vulnerable aspect in the security framework of 5G-railway(5G-R)system.To enhance the control of 5G-R terminals and their access to applications,this paper analyzes the applicatio... Terminals and their access represent a vulnerable aspect in the security framework of 5G-railway(5G-R)system.To enhance the control of 5G-R terminals and their access to applications,this paper analyzes the application scenarios,operational modes,services supported by 5G-R terminals,and the data paths between these terminals and the connected railway application service systems.Further analysis concentrates on the security risks posed by the characteristics of intelligent 5G-R handheld terminals,lightweight Internet of Things(IoT)communication terminals,and onboard integrated wireless transmission equipment with public-private convergence.In light of the risks above,this paper presents the terminal security control requirements.Furthermore,based on the planned architecture of the 5G-R system and security technologies such as terminal identity authentication and behavior auditing,the paper proposes a solution package for the 5G-R terminal security control system,including the overall architecture,functional implementation,and interface configuration.These solutions aim to achieve unified control over the admission and access of 5G-R handheld terminals,IoT communication terminals,and onboard integrated wireless communication equipment to railway application systems.Additionally,they enable the security control and analysis of terminal behaviors and application data,facilitate the security management of terminals,and ensure the secure release,download,and installation of mobile applications. 展开更多
关键词 5G-R 5G-R terminal application access security control communication interface
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Urban geological information platform for smart city construction:A shift from public service to integration with urban engineering
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作者 Huaixue Xing Bofan Yu +5 位作者 li hui Weiya Ge Wen Zhou Jian Hua Congxuan Kang Yan Zou 《Intelligent Geoengineering》 2025年第2期80-95,共16页
Urban geological information platforms have traditionally focused on static data provision for public service,constrained by funding and limited engagement with engineering applications.This study takes Hangzhou-a maj... Urban geological information platforms have traditionally focused on static data provision for public service,constrained by funding and limited engagement with engineering applications.This study takes Hangzhou-a major Chinese megacity-as a model to propose a technically integrated platform that aligns with urban infrastructure development,particularly underground space engineering.Through the adoption of the large-scale relational database system Oracle,we first established a comprehensive storage framework for fundamental urban geological and underground infrastructure information,thereby completed the construction of the core databases.To ensure spatial consistency across multi-source data and to meet the platform’s high computational demands while improving overall server responsiveness,we introduced three critical innovations:voxel-based model encoding,distributed computing,and frontend-backend separation with asynchronous processing.To align with urban engineering projects and enhance economic returns,the platform was initially developed through the integration of foundational geological data,including borehole records and aboveground-underground spatial information.Based on this foundation,its practical application in Hangzhou’s Qiantang New Town further demonstrated the platform’s potential in supporting subway routing,underground structure planning,and engineering cost analysis.Consequently,the construction of the Hangzhou geological information platform not only offers robust support for urban decision-making and smart city development but also provides a replicable model for addressing the technical and institutional challenges commonly encountered in the development of urban geological platforms. 展开更多
关键词 Urban geology Geological database Geology information platform Underground construction Smart city
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Basic Properties,Mechanical Properties,Long-Term Durability and Application Prospects of Magnesium Slag Materials
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作者 ZHANG Lu XU Zhiming +3 位作者 li hui NIU Ditao ZHOU Yinuo YAN Ziwei 《Journal of Wuhan University of Technology(Materials Science)》 2025年第5期1434-1449,共16页
By using the phased characteristics summarizing method of the existing research on magnesium slag,this study investigates the hydration reaction,alkali activation reaction and CO_(2) mineralization reaction processes ... By using the phased characteristics summarizing method of the existing research on magnesium slag,this study investigates the hydration reaction,alkali activation reaction and CO_(2) mineralization reaction processes and mechanisms,and then explores its high-value utilization.The results show that physical and chemical activation can improve the mechanical properties of the gelled material system by increasing the crystal phase defects and surface energy and by reconstructing a new gelling system by depolymerizing glass.The CO_(2) mineralization reaction of magnesium slag can be used to construct a new gelling system for CaCO_(3) and calcium-modified silica gel.Magnesium slag can also be used to enhance the dry shrinkage and carbonation resistance of concrete owing to its expansibility and high alkali reserves.The mechanism and existence form of heavy metal ions in magnesium slag have been clarified.The study proposed a production system for magnesium slag and highlighted the potential research value in the field of wet carbonation to promote the application of magnesium slag. 展开更多
关键词 magnesium slag CO_(2)mineralization solid waste mechanical properties DURABILITY
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Alleviating Poverty Like China:Evidence from the Poverty Alleviation and Development Plans
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作者 li hui Zhang Ziyao Yin Heng 《China Economist》 2025年第5期2-23,共22页
Amid China’s rapid economic growth,significant regional imbalances persisted.To address this disparity,it is essential to pursue high-quality development by fostering endogenous drivers within poverty-stricken areas.... Amid China’s rapid economic growth,significant regional imbalances persisted.To address this disparity,it is essential to pursue high-quality development by fostering endogenous drivers within poverty-stricken areas.In late 2011,the Chinese government introduced a new nationwide regional poverty alleviation and development program.This study examines the macroeconomic impacts and micro-level mechanisms of these policies by leveraging manually compiled policy documents and macro and micro data(county economies,enterprises,and land transactions),using a DID approach.The findings reveal that the program significantly boosted economic growth in poor regions without causing negative spillovers to neighboring or paired-assistance areas.Economic growth in the assisted regions was primarily driven by industrialization,encompassing both scale expansion and efficiency gains.A cost-benefit analysis further confirms substantial economic returns and long-term fiscal sustainability.These results provide important policy implications for refining regional poverty policies and advancing balanced development. 展开更多
关键词 Regional poverty policy High-quality development Difference-in-Differences(DID)
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LiFePO_(4)正极材料掺杂改性研究进展
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作者 李辉 李尉新 +3 位作者 吴立群 陈振绳 李意能 李顺艳 《云南化工》 2025年第8期23-27,共5页
正极材料是影响锂离子电池关键性能的核心材料,而橄榄石结构的磷酸铁锂(LFP)因其具有容量大、循环寿命长、成本低和安全等优点,被公认为是一种很有前途的正极材料。然而,LFP材料自身的晶体结构导致了该材料存在电子电导率差、离子扩散... 正极材料是影响锂离子电池关键性能的核心材料,而橄榄石结构的磷酸铁锂(LFP)因其具有容量大、循环寿命长、成本低和安全等优点,被公认为是一种很有前途的正极材料。然而,LFP材料自身的晶体结构导致了该材料存在电子电导率差、离子扩散系数小和压实密度低等问题,严重制约了其在锂离子动力电池中的进一步应用。如何进一步提升LFP正极材料的性能,是目前研究的重点领域之一。综述了LFP正极材料现有的掺杂改性技术,同时对LFP未来在商业化中的改良和开发等发展前景面临的挑战进行了展望。 展开更多
关键词 磷酸铁锂(LFP) 正极材料 掺杂改性
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基于AMESim/Simulink的补燃循环火箭发动机与调节阀特性仿真 被引量:3
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作者 李辉 郭迎清 +1 位作者 徐柯杰 闫星辉 《航空动力学报》 EI CAS CSCD 北大核心 2024年第1期169-176,共8页
在AMESim软件环境下重新建立了某型补燃循环液体火箭发动机的调节阀部件动态模型,在Simulink软件环境下建立了机电作动系统的动态模型,将两者与修改后的火箭发动机模型进行变推力过程联合仿真,并在仿真中注入输送管路压力扰动。仿真结... 在AMESim软件环境下重新建立了某型补燃循环液体火箭发动机的调节阀部件动态模型,在Simulink软件环境下建立了机电作动系统的动态模型,将两者与修改后的火箭发动机模型进行变推力过程联合仿真,并在仿真中注入输送管路压力扰动。仿真结果表明:机电作动系统与调节阀模型能够反映发动机变推力过程中各部件内部的参数变化;联合仿真保持了原发动机模型的稳态精度,各主要参数误差均在1‰量级;调节阀的活塞自反馈机构能够抑制输送管路中低频压力波动对发动机推力的影响。 展开更多
关键词 液体火箭发动机 调节阀 电动执行机构 AMESIM软件 Simulink软件 联合仿真
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基于氢能固态运输的电-氢综合能源系统双层调度模型 被引量:5
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作者 谭洪 王宇炜 +2 位作者 王秋杰 李辉 李振兴 《电工技术学报》 北大核心 2025年第3期744-758,共15页
中短距离氢能运输需求下,长管拖车运氢具有较好的经济性,但长管拖车的储氢密度低,且高压氢气存在爆炸和泄漏等风险。为此,该文提出一种基于氢能固态运输的电-氢综合能源系统(EHIES)双层调度模型。首先,通过分析金属固态储氢机理,并利用... 中短距离氢能运输需求下,长管拖车运氢具有较好的经济性,但长管拖车的储氢密度低,且高压氢气存在爆炸和泄漏等风险。为此,该文提出一种基于氢能固态运输的电-氢综合能源系统(EHIES)双层调度模型。首先,通过分析金属固态储氢机理,并利用氢能气-固两相转换过程中压强与反应温度关系,构建氢能固态运载车(HSTV)的装卸模型;其次,采用改进含时间窗车辆路径问题构建HSTV的运输模型;最后,基于隶属度的信息间隙决策理论(M-IGDT)建立EHIES日前双层调度模型,并将所提双层模型转换成单层模型求解。采用改进的IEEE 118和IEEE300系统进行仿真,结果表明氢能固态运输能有效地提升氢能运输效率和系统经济性。 展开更多
关键词 电-氢综合能源系统 氢能固态运输 信息间隙决策理论 双层调度
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中扬子西部下古生界页岩沉积古环境 被引量:1
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作者 沈均均 杨丽亚 +5 位作者 王玉满 李辉 王鹏万 周林 刘计勇 孟江辉 《沉积学报》 北大核心 2025年第1期314-334,共21页
【目的】下古生界筇竹寺组和龙马溪组是中上扬子地区页岩气勘探开发的重点层系,而对两套页岩有机质富集的控制因素一直缺乏系统对比研究。【方法】以中扬子西部两套海相页岩为例,对其在被动大陆边缘和克拉通坳陷内沉积学及地球化学特征... 【目的】下古生界筇竹寺组和龙马溪组是中上扬子地区页岩气勘探开发的重点层系,而对两套页岩有机质富集的控制因素一直缺乏系统对比研究。【方法】以中扬子西部两套海相页岩为例,对其在被动大陆边缘和克拉通坳陷内沉积学及地球化学特征进行对比分析,探讨有机质差异富集主控因素及形成模式。【结果】两套页岩高总有机碳(Total Organic Carbon,TOC)段均位于底部,形成于陆源输入量低、古生产力水平高和保存条件好的环境,且筇竹寺组底部古生产力水平和底水还原程度更高,导致其TOC含量更高。此后,筇竹寺期盆地拉张裂陷作用持续减弱,龙马溪期前陆挠曲—迁移作用逐渐增强,两套页岩均表现出陆源输入量增大和保存条件变差的特点,但古生产力水平变化却截然不同:筇竹寺期,洋流活动随着海平面下降而减弱,古生产力水平随之降低,而龙马溪期,挠曲—迁移作用导致扬子海盆北部障壁开口逐渐打开,洋流涌入规模逐渐扩大,受海平面下降影响较弱,古生产力水平随之升高。因此,纵向上,筇竹寺组TOC含量降低主要受古生产力水平降低、保存条件变差和陆源输入量增大共同控制,而龙马溪组则主要受后两个因素控制。横向上,两套页岩由被动大陆边缘过渡至克拉通坳陷内,海平面高度和洋流活跃程度均呈降低趋势,保存条件和古生产力水平同时变差,TOC含量随之降低。受此控制,筇竹寺组页岩气勘探开发重点应向西南落实,而龙马溪组则应转向西北方向,二者难以同时兼顾。【结论】该研究对于深入理解下古生界海相页岩沉积古环境演化特征、有机质富集机理及指导页岩气勘探实践均具有积极意义。 展开更多
关键词 笻竹寺组 龙马溪组 黑色页岩 有机质 沉积古环境 富集主控因素
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苏州市34所医疗机构全自动内镜清洗消毒机清洗和消毒效果评价 被引量:1
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作者 赵瑶 郭新怡 +7 位作者 杨波 陆建英 汪茜雅 金美娟 丁蔚 李慧 许圆 孙湘 《中华医院感染学杂志》 北大核心 2025年第2期281-285,共5页
目的了解医疗机构全自动内镜清洗消毒机(AER)的清洗效果和消毒效果,以期为医疗机构在使用和维护AER上提供数据支持。方法2023年9月—2024年4月对苏州市所有配备AER的医疗机构进行现场采样和检测,使用三磷酸腺苷(ATP)生物荧光检测法检测... 目的了解医疗机构全自动内镜清洗消毒机(AER)的清洗效果和消毒效果,以期为医疗机构在使用和维护AER上提供数据支持。方法2023年9月—2024年4月对苏州市所有配备AER的医疗机构进行现场采样和检测,使用三磷酸腺苷(ATP)生物荧光检测法检测AER清洗前后内镜表面和附件的清洗效果,使用蛋白残留法检测AER清洗前后内镜管腔的残留蛋白量,使用滤膜法检测内镜的消毒效果和AER内终末漂洗用水的情况,比较AER和手工两种方式消毒对内镜合格率的影响。结果共检测了34家医疗机构,43条消化内镜(胃镜27根,肠镜16根),内镜在AER清洗前后管腔的蛋白残留量平均值为51.82μg、35.38μg,表面ATP检测平均值为214 RLU、64 RLU,附件ATP检测平均值为126 RLU、32 RLU。ATP评价胃镜表面清洗前后合格率、内镜附件清洗前后合格率有统计学差异(P<0.05),管腔的蛋白残留在清洗前后未发现差别。使用AER消毒胃镜和肠镜的合格率分别为96.30%和87.50%,手工清洗消毒的胃镜和肠镜的合格率分别为100.00%和87.50%,使用AER和手工两种方式对消化内镜消毒后合格率未发现统计学差异。AER和手工的终末漂洗用水合格率分别为87.50%、80.00%。结论三级医疗机构的AER配置率较高,使用AER和手工两种方式对消化内镜消毒的效果无统计学差异,ATP生物荧光检测法和蛋白残留法评价AER清洗消化内镜效果尚可。建议多关注使用AER消毒的内镜和AER终末漂洗用水质量的定期监测。 展开更多
关键词 内镜 全自动内镜清洗消毒机 清洗消毒效果 三磷酸腺苷生物荧光检测法 蛋白残留法 终末漂洗用水
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外科治疗联合英夫利昔单抗与乌司奴单抗克对罗恩肛瘘的临床疗效观察 被引量:1
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作者 袁和学 田丰 +6 位作者 李卉 罗芳 赵亮 刘宗剑 潘春来 刘利军 朱娜 《实用医学杂志》 北大核心 2025年第6期872-876,共5页
目的研究克罗恩肛瘘(perianal fistulizing Crohn's disease,pfCD)外科治疗联合生物制剂的临床疗效观察。方法采用回顾性病例对照研究,选取医院2021年5月至2023年12月收治的pf CD患者60例,随机分为治疗组A(n=30)和治疗组B(n=30),治... 目的研究克罗恩肛瘘(perianal fistulizing Crohn's disease,pfCD)外科治疗联合生物制剂的临床疗效观察。方法采用回顾性病例对照研究,选取医院2021年5月至2023年12月收治的pf CD患者60例,随机分为治疗组A(n=30)和治疗组B(n=30),治疗组A患者采用外科治疗+英夫利昔单抗(IFX)+硫唑嘌呤(AZA),治疗组B患者采用外科治疗+乌司奴单抗(UST)+AZA,观察两组患者克罗恩病活动指数(CDAI)、肛周疾病活动指数(PDAI)及Assche评分,术后MRI检查,观察pf CD愈合状况。结果治疗组A与B的治疗前与治疗后比较,CDAI、PDAI、Assche评分差异有统计学意义(P<0.05)且各时间点的CDAI、PDAI、Assche评分变化量间均有差异。治疗组A与B,8、16、24、32周的CDAI、PDAI、Assche评分及40周的PDAI评分比较差异无统计学意义(P>0.05),而40周的CDAI及Assche评分比较差异有统计学意义(P<0.05)。结论外科治疗联合IFX/UST治疗pf CD安全有效,但外科治疗联合UST远期疗效更好。 展开更多
关键词 克罗恩肛瘘 外科治疗 英夫利昔单抗 乌司奴单抗
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青年缺血性脑卒中病人血清miR-218-5p、LASP1水平及其应用价值 被引量:1
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作者 亓超 李慧 +1 位作者 吴永亚 李晨曦 《安徽医药》 CAS 2025年第1期156-159,共4页
目的探究青年缺血性脑卒中(IS)病人血清微RNA-218-5p(miR-218-5p)、LIM和SH3蛋白1(LASP1)水平及其应用价值。方法选取2020年6月至2022年6月山东中医药大学附属医院收治的青年IS病人96例为IS组,对所有IS病人进行为期3个月的随访,按照改良... 目的探究青年缺血性脑卒中(IS)病人血清微RNA-218-5p(miR-218-5p)、LIM和SH3蛋白1(LASP1)水平及其应用价值。方法选取2020年6月至2022年6月山东中医药大学附属医院收治的青年IS病人96例为IS组,对所有IS病人进行为期3个月的随访,按照改良Rankin量表(mRS)评分进行分组,预后良好组68例(mRS评分≤2分)和预后不良组28例(mRS评分>2分)。选择同期在该院进行体检的健康志愿者96例为对照组。血清miR-218-5p、LASP1 mRNA水平检测采用实时荧光定量PCR(qRT-PCR);Pearson相关性分析血清miR-218-5p与LASP1 mRNA表达水平的关系。采用受试者操作特征曲线(ROC曲线)分析血清中miR-218-5p、LASP1 mRNA表达水平对IS预后评估的价值。结果与对照组相比,IS组白细胞计数、总胆固醇、三酰甘油、低密度脂蛋白胆固醇水平显著升高,高密度脂蛋白胆固醇水平显著降低(P<0.05);与对照组(1.03±0.11、1.01±0.11)相比,IS组血清中miR-218-5p水平0.88±0.09显著降低,LASP1 mRNA(1.12±0.12)水平显著升高(P<0.05)。IS病人血清miR-218-5p与LASP1 mRNA呈负相关(r=−0.73,P<0.001)。与预后良好组(0.94±0.10、1.05±0.11)相比,预后不良组血清中miR-218-5p(0.74±0.08)水平显著降低,LASP1 mRNA(1.28±0.13)水平显著升高(P<0.05)。ROC曲线显示,二者联合评估IS预后不良的AUC高于miR-218-5p、LASP1 mRNA单独预测的AUC值(Z=12.35,P<0.001;Z=6.60,P=0.010)。结论青年IS病人血清miR-218-5p较低,LASP1 mRNA较高,可用于评估青年IS病人的预后。 展开更多
关键词 卒中 脑梗死 青年 微核糖核酸-218-5p LIM和SH3蛋白1 预后
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装配式PVA-ECC梁柱节点抗震性能研究 被引量:1
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作者 李慧 杨丹 +1 位作者 郭瑞 余志祥 《建筑结构》 北大核心 2025年第4期38-43,共6页
节点核心区是装配式梁柱节点的薄弱部分,改善节点的连接是增强装配式结构核心区抗剪性能的关键问题。为提高装配式梁柱节点的抗震性能,采用聚乙烯醇-工程水泥基复合材料(PVA-ECC)代替梁柱节点核心区及相邻一定范围内梁端与柱端的普通混... 节点核心区是装配式梁柱节点的薄弱部分,改善节点的连接是增强装配式结构核心区抗剪性能的关键问题。为提高装配式梁柱节点的抗震性能,采用聚乙烯醇-工程水泥基复合材料(PVA-ECC)代替梁柱节点核心区及相邻一定范围内梁端与柱端的普通混凝土,并提出一种装配式梁柱节点的拆分构造。通过3个梁柱节点在循环荷载作用下的试验结果,比较了不同构造下梁柱节点的滞回性能、承载能力、耗能能力及刚度退化等抗震性能指标,验证了拆分构造方式的可行性。试验结果表明:现浇混凝土试件破坏最为严重,装配式PVA-ECC梁柱节点无混凝土剥落现象且裂缝宽度较小,并表现出多裂缝开裂现象;对比结果表明,纤维增强梁柱节点承载能力增强,可实现“等同现浇”,刚度退化减缓,耗能能力增强18%左右,表明提出的装配式PVA-ECC梁柱节点的拆分构造方式可行;通过对梁柱节点进行承载力验算,验证了装配式PVA-ECC梁柱节点的合理性,可进行实际应用。 展开更多
关键词 聚乙烯醇-工程水泥基复合材料 装配式梁柱节点 多裂缝开裂 拆分构造
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产业转型升级示范区的绿色转型效应--基于“数产融合”视角 被引量:3
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作者 李慧 佟孟华 +1 位作者 张国建 赵江山 《财经研究》 北大核心 2025年第1期4-18,共15页
协同推进“数产融合”发展是把握新一轮科技革命和产业变革新机遇的重要战略选择,也是实现经济高质量发展的必然要求。因此,文章基于2007—2021年城市层面的数据,采用三重差分模型考察了数字经济促进示范区城市绿色转型的异质性效应。... 协同推进“数产融合”发展是把握新一轮科技革命和产业变革新机遇的重要战略选择,也是实现经济高质量发展的必然要求。因此,文章基于2007—2021年城市层面的数据,采用三重差分模型考察了数字经济促进示范区城市绿色转型的异质性效应。研究发现:协同推进“数产融合”发展能够有效提升资源型城市的绿色全要素生产率水平。该结论在考虑内生性问题以及进行一系列稳健性检验和识别假定检验后依然成立。机制分析表明,数字经济促进示范区城市绿色转型的促进作用主要来自绿色技术效率的提升;同时,“数产融合”主要通过缓解资本错配和促进传统产业转型升级的方式推动资源型城市绿色转型发展,而劳动力供需不匹配导致的市场扭曲问题阻碍了资源型城市的绿色创新发展,这是导致示范区城市绿色技术进步水平较低的原因。进一步研究发现,数字经济促进示范区城市绿色转型具有显著的空间溢出效应和非线性特征;此外,协同推进“数产融合”发展是资源型城市打破“资源诅咒”问题的关键,但数字经济发展的不平衡导致了中心城市与外围城市间的“数字鸿沟”。文章的研究为加快推进传统产业数字化转型和解决“数字鸿沟”难题提供了重要启示。 展开更多
关键词 “数产融合”发展 绿色全要素生产率 “数字鸿沟”
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基于非均匀源活度分布反演的γ辐射场重构方法 被引量:2
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作者 刘立业 樊清 +3 位作者 李华 李会 靳海晶 赵原 《核技术》 北大核心 2025年第4期80-89,共10页
辐射场的准确重构是实现辐射防护数字化的基础,也是进行辐射剂量评估与可视化仿真的前提。为了有效解决目前核设施现场由于高梯度非均匀辐射源项分布导致的三维γ辐射场重构偏差较大的切实问题,本文创新性地提出基于贝叶斯信息准则建立... 辐射场的准确重构是实现辐射防护数字化的基础,也是进行辐射剂量评估与可视化仿真的前提。为了有效解决目前核设施现场由于高梯度非均匀辐射源项分布导致的三维γ辐射场重构偏差较大的切实问题,本文创新性地提出基于贝叶斯信息准则建立的非均匀源活度分布模型择优方法,并将最优模型用于三维γ辐射场的重构。结果表明:该方法在高活度梯度、高剂量率梯度情形下的辐射场重构准确度显著优于克里金插值方法;在核设施实际场景的三维辐射场重构案例中,测试集剂量率重构结果与实测值平均相对偏差仅为12.69%,低于克里金插值结果的85.40%。本研究可为核设施现场基于动态数据驱动的辐射防护数字化仿真工作提供先进技术支撑,增强其在实际应用中的效果。 展开更多
关键词 三维辐射场 源活度反演 非均匀分布 信息准则 辐射防护
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低氧条件下丹栀逍遥散对乳腺癌细胞增殖侵袭及缺氧相关因子的影响 被引量:2
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作者 李慧 李然 《时珍国医国药》 北大核心 2025年第4期651-657,共7页
目的研究丹栀逍遥散含药血清在低氧条件下对乳腺癌MDA-MB-231细胞增殖、侵袭及缺氧相关因子表达的影响。方法噻唑蓝比色法(MTT)检测细胞增殖,将MDA-MB-231细胞分为常氧空白组、低氧空白组、丹栀逍遥散组(含药血清10%、15%、20%),培养24,... 目的研究丹栀逍遥散含药血清在低氧条件下对乳腺癌MDA-MB-231细胞增殖、侵袭及缺氧相关因子表达的影响。方法噻唑蓝比色法(MTT)检测细胞增殖,将MDA-MB-231细胞分为常氧空白组、低氧空白组、丹栀逍遥散组(含药血清10%、15%、20%),培养24,48,72h,观察药物对细胞增殖的抑制作用;通过划痕实验观察各组细胞迁移侵袭情况;蛋白免疫印迹法(WB)检测各组低氧诱导因子-1α(HIF-1α)、单羧酸转运蛋白1/4(MCT1/4)、小窝蛋白-1(CAV-1)及趋化因子受体4(CXCR4)的蛋白表达情况。结果MTT结果表明,低氧条件下,丹栀逍遥散组可显著抑制细胞增殖(P<0.05),其中20%含药血清孵育48h抑制率最佳。划痕实验结果表明,丹栀逍遥散组(20%)细胞迁移率明显下降(P<0.01)。WB实验结果表明,低氧条件下,丹栀逍遥散组(20%)的HIF-1α、MCT1、MCT4、CXCR4的蛋白表达降低(P<0.05、P<0.01)、CAV-1的表达升高(P<0.05)。结论丹栀逍遥散含药血清在低氧条件下能明显抑制MDA-MB-231乳腺癌细胞增殖、迁移侵袭,发挥抗癌作用,可能与其调控缺氧相关乳酸代谢及上皮间质转化(EMT)有关。 展开更多
关键词 乳腺癌 丹栀逍遥散 低氧 增殖 侵袭
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