Background:Hepatocellular carcinoma(HCC)is a highly lethal malignancy driven by both intrinsic oncogenic pathways and immune microenvironmental regulation.Emerging evidence suggests that DNASE1L3 may influence tumor b...Background:Hepatocellular carcinoma(HCC)is a highly lethal malignancy driven by both intrinsic oncogenic pathways and immune microenvironmental regulation.Emerging evidence suggests that DNASE1L3 may influence tumor biology and immune responses;however,its specific roles in HCC progression and macrophage-mediated regulation remain unclear.This study aimed to elucidate the biological functions of DNASE1L3 in HCC and to determine how it modulates tumor behavior and immune interactions.Methods:Bioinformatics analyses of the GSE41804 and Cancer Genome Atlas-Liver Hepatocellular Carcinoma(TCGA-LIHC)datasets were used to identify hub genes.Functional assays assessed the impact of DNASE1L3 on HCC cell proliferation,migration,invasion,and cell cycle progression.The effects of DNASE1L3 on macrophage polarization and the Wnt/β-catenin signaling pathway were examined using a co-culture system.An HCC organoid model was established to further validate its regulatory function.Results:Eight prognostic signature genes were identified,with deoxyribonuclease I-like 3(DNase I-like 3)selected as the hub gene.DNASE1L3 overexpression suppressed HCC cell growth,inhibited migration and invasion,induced G1 arrest,and modulated epithelial-mesenchymal transition(EMT)markers.DNASE1L3 knockdown promoted M2-like macrophage polarization.Mechanistically,DNASE1L3 interacted withβ-catenin to enhance its ubiquitination and degradation,thereby inhibiting Wnt/β-catenin signaling and reducing PD-L1 expression.DNASE1L3 overexpression similarly restricted organoid growth and suppressed pathway activity.Conclusion:DNASE1L3 acts as a negative regulator of HCC progression by targeting the Wnt/β-catenin pathway and reducing PD-L1 expression,thereby influencing both tumor cell behavior and macrophage-mediated immune responses.展开更多
In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E.coli)K88,microorganisms were screened from the feces of Min pigs,and their antibacterial and biological...In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E.coli)K88,microorganisms were screened from the feces of Min pigs,and their antibacterial and biological characteristics were studied.Using E.coli K88 as the indicator bacterium,the supernatant of the isolated strain from Min pigs was co-cultured with the indicator bacteria for the antibacterial test.The Bacillus velezensis(B.velezensis)strain MZ-78 showed stronger antimicrobial activity against E.coli K88 than other isolates,and its supernatant inhibited the growth of other pathogens including Salmonella typhimurium(S.typhimurium),E.coli,Staphylococcus aureus(S.aureus),and Pseudomonas aeruginosa(P.aeruginosa).Further studies showed that the selected strain MZ-78 exhibited a certain tolerance to sodium chloride conditions.The B.velezensis strain MZ-78 exhibited no hemolytic activity and was not susceptible to antibiotics.Additionally,during metabolism,it generated a certain quantity of short-chain fatty acids,including isobutyric acid and isovaleric acid.Furthermore,this strain demonstrated the capability to counteract the adhesion of E.coli K88 to porcine small intestinal epithelial cells(IPEC-J2),while also promoting their growth and proliferation.The B.velezensis strain MZ-78 isolated from Min pigs feces showed a series of probiotic characteristics and the potential to survive in the digestive tract of animals,which indicated its potential commercial application value as a probiotic alternative to antibiotics in animal husbandry.展开更多
The in-flight calibration and performance of the Solar Disk Imager(SDI),which is a pivotal instrument of the LyαSolar Telescope onboard the Advanced Space-based Solar Observatory mission,suggested a much lower spatia...The in-flight calibration and performance of the Solar Disk Imager(SDI),which is a pivotal instrument of the LyαSolar Telescope onboard the Advanced Space-based Solar Observatory mission,suggested a much lower spatial resolution than expected.In this paper,we developed the SDI point-spread function(PSF)and Image Bivariate Optimization Algorithm(SPIBOA)to improve the quality of SDI images.The bivariate optimization method smartly combines deep learning with optical system modeling.Despite the lack of information about the real image taken by SDI and the optical system function,this algorithm effectively estimates the PSF of the SDI imaging system directly from a large sample of observational data.We use the estimated PSF to conduct deconvolution correction to observed SDI images,and the resulting images show that the spatial resolution after correction has increased by a factor of more than three with respect to the observed ones.Meanwhile,our method also significantly reduces the inherent noise in the observed SDI images.The SPIBOA has now been successfully integrated into the routine SDI data processing,providing important support for the scientific studies based on the data.The development and application of SPIBOA also paves new ways to identify astronomical telescope systems and enhance observational image quality.Some essential factors and precautions in applying the SPIBOA method are also discussed.展开更多
在“双碳”目标驱动下,综合能源系统(integrated energy system,IES)参与电-碳市场协同优化面临自愿减排激励不足与跨主体利益分配失衡的双重挑战,亟须构建兼顾经济性、低碳性与公平性的市场交易机制。提出一种综合能源系统自愿碳减排的...在“双碳”目标驱动下,综合能源系统(integrated energy system,IES)参与电-碳市场协同优化面临自愿减排激励不足与跨主体利益分配失衡的双重挑战,亟须构建兼顾经济性、低碳性与公平性的市场交易机制。提出一种综合能源系统自愿碳减排的电-碳市场投标竞价与利益分配机制。基于核密度估计与Wasserstein距离生成风光不确定性场景,考虑风电及光伏出力预测误差对电-碳市场投标的影响,建立了三阶段电-碳市场投标竞价与利益分配方法。第一阶段为日前电-碳预投标阶段,基于风光出力的预测值进行预投标,获得预期投标量与预计碳排放。第二阶段为日内调度阶段,考虑IES间的合作博弈与能源共享,确定模糊集内最坏情况分布中最小化调度成本。第三阶段为利益分配阶段,采用Myerson值与Shapley值分配方法分配收益,同时考虑碳转移、碳抵消、碳补偿等多种碳减排机制,激励IES减少系统碳排放,减少投标成本。最后以某工业园区为例,验证了自愿碳减排的电-碳市场投标竞价与利益分配方法的有效性。展开更多
Transition metal catalysts supported on oxides undergo significant structural changes under reaction conditions,which are influenced by interactions with reactants.These interactions can lead to the dispersion of meta...Transition metal catalysts supported on oxides undergo significant structural changes under reaction conditions,which are influenced by interactions with reactants.These interactions can lead to the dispersion of metal atoms,leading to the formation of stable single-nucleus metal complexes.Understanding the stability of these complexes is essential for catalyst design.In this study,we use multitask symbolic regression to identify a descriptor for the stability of singlenucleus metal complexes with common reactants,such as CO and H_(2)O,on the basis of first-principles calculations.We develop a multidimensional descriptor incorporating metal‒metal,metal‒support,and metal‒adsorbate interactions,achieving high accuracy in predicting the stability of single-nucleus metal complexes.Our analysis revealed that in the absence of reactants,the stability of single metal atoms is mainly determined by the hardness(cohesive energy)of the metal.The presence of reactants such as CO and H_(2)O further stabilizes single-nucleus metal complexes by saturating undercoordinated metal atoms.This stabilization is correlated with the Lewis acidity of the surface oxygen in the support.Supports with lower Lewis acidity enhance metal‒support interactions,promoting CO adsorption on all metals and H_(2)O adsorption on complexes of hard metals.In contrast,supports with higher Lewis acidity enhance hydrogen adsorption,promoting H_(2)O interactions with complexes of soft metals as well as Pd and Pt.Additionally,our descriptor predicts nanoparticle(NP)dissociation into single-nucleus metal complexes or Ostwald ripening(OR)tendencies.Under CO conditions,harder metals tend to favor dissociation,whereas softer metals(e.g.,Ag,Cu,and Au)are more prone to OR.Under H_(2)O conditions,the Lewis acidity of the support surface oxygen influences NP behavior,with supports such as CeO_(2)stabilizing single-nucleus metal complexes and promoting NP dissociation.These insights provide guidance for selecting catalyst components and optimizing reaction conditions to control the stability of single-nucleus metal complexes and guide NP dissociation or OR processes.展开更多
The use of lithium-sulfur(Li-S)batteries is limited by sulfur redox reactions involving multi-phase transformations,especially at low-temperatures.To address this issue,we report a material(FCNS@NCFs)consisting of nit...The use of lithium-sulfur(Li-S)batteries is limited by sulfur redox reactions involving multi-phase transformations,especially at low-temperatures.To address this issue,we report a material(FCNS@NCFs)consisting of nitrogen-doped carbon fibers loaded with a ternary metal sulf-ide((Fe,Co,Ni)_(9)S_(8))for use as the sulfur host in Li-S batteries.This materi-al was prepared using transfer blot filter paper as the carbon precursor,thiourea as the source of nitrogen and sulfur,and FeCl_(3)·6H_(2)O,CoCl_(2)·6H_(2)O and NiCl_(2)·6H_(2)O as the metal ion sources.It was synthesized by an impreg-nation method followed by calcination.The nitrogen doping significantly in-creased the conductivity of the host,and the metal sulfides have excellent catalytic activities.Theoretical calculations,and adsorption and deposition experiments show that active sites on the surface of FCNS@NCFs selectively adsorb polysulfides,facilitate rapid adsorption and conversion,prevent cathode passivation and inhib-it the polysulfide shuttling.The FCNS@NCFs used as the sulfur host has excellent electrochemical properties.Its initial dis-charge capacity is 1639.0 mAh g^(−1) at 0.2 C and room temperature,and it remains a capacity of 1255.1 mAh g^(−1) after 100 cycles.At−20~C,it has an initial discharge capacity of 1578.5 mAh g^(−1) at 0.2 C,with a capacity of 867.5 mAh g^(−1) after 100 cycles.Its excellent performance at both ambient and low temperatures suggests a new way to produce high-performance low-temper-ature Li-S batteries.展开更多
To obtain the outlet temperature of combustor,a kind of high-temperature and water cooling thermocouple was designed.The main factors affecting the results of thermocouple measurement were analyzed after numerical sim...To obtain the outlet temperature of combustor,a kind of high-temperature and water cooling thermocouple was designed.The main factors affecting the results of thermocouple measurement were analyzed after numerical simulation.Results showed that the high-temperature water cooling thermocouple can achieve high temperature measurement under the condition of 2 400 K.With the increase of the distance between the water cooling structure and the stagnation cover,the temperature measurement result was more accurate,and the increase in the area ratio of the inlet and outlet of the stagnation cover within a reasonable range can make the measurement results more accurate.The surface emissivity of the measuring point had a great influence on the radiation error.The pressure and flow rate of cooling water can be effectively reduced after adding zirconia coating onto the surface of the rake body.展开更多
现有基于虚拟正弦信号注入(virtual sine signal injection,VSSI)的最大转矩电流比(maximum torque per ampere,MTPA)控制方法以理想永磁同步电动机(permanent magnet synchronous machine,PMSM)数学模型为理论基础,未考虑电感变化带来...现有基于虚拟正弦信号注入(virtual sine signal injection,VSSI)的最大转矩电流比(maximum torque per ampere,MTPA)控制方法以理想永磁同步电动机(permanent magnet synchronous machine,PMSM)数学模型为理论基础,未考虑电感变化带来的不利影响。为了提升MTPA控制效果,增加考虑磁路交叉耦合效应,提出在线电感辨识方法和改进的MTPA控制方法。基于帕德逼近与变步长神经网络的电感辨识方法,不仅可以解决辨识模型欠秩问题,而且能够在线更新变化的电感参数,结合改进的基于VSSI的MTPA控制,可以有效降低电流矢量角误差,减小定子铜损耗,实现更准确的MTPA控制。通过仿真实验对所提方法的准确性进行了验证。展开更多
基金funded by Shanghai Science and Technology Innovation Action Plan Project(22140901100)Shanghai Key Laboratory of Molecular Imaging(18DZ2260400)Shanghai University of Medicine and Health Science Seed Fund(SSF-24-21-01).
文摘Background:Hepatocellular carcinoma(HCC)is a highly lethal malignancy driven by both intrinsic oncogenic pathways and immune microenvironmental regulation.Emerging evidence suggests that DNASE1L3 may influence tumor biology and immune responses;however,its specific roles in HCC progression and macrophage-mediated regulation remain unclear.This study aimed to elucidate the biological functions of DNASE1L3 in HCC and to determine how it modulates tumor behavior and immune interactions.Methods:Bioinformatics analyses of the GSE41804 and Cancer Genome Atlas-Liver Hepatocellular Carcinoma(TCGA-LIHC)datasets were used to identify hub genes.Functional assays assessed the impact of DNASE1L3 on HCC cell proliferation,migration,invasion,and cell cycle progression.The effects of DNASE1L3 on macrophage polarization and the Wnt/β-catenin signaling pathway were examined using a co-culture system.An HCC organoid model was established to further validate its regulatory function.Results:Eight prognostic signature genes were identified,with deoxyribonuclease I-like 3(DNase I-like 3)selected as the hub gene.DNASE1L3 overexpression suppressed HCC cell growth,inhibited migration and invasion,induced G1 arrest,and modulated epithelial-mesenchymal transition(EMT)markers.DNASE1L3 knockdown promoted M2-like macrophage polarization.Mechanistically,DNASE1L3 interacted withβ-catenin to enhance its ubiquitination and degradation,thereby inhibiting Wnt/β-catenin signaling and reducing PD-L1 expression.DNASE1L3 overexpression similarly restricted organoid growth and suppressed pathway activity.Conclusion:DNASE1L3 acts as a negative regulator of HCC progression by targeting the Wnt/β-catenin pathway and reducing PD-L1 expression,thereby influencing both tumor cell behavior and macrophage-mediated immune responses.
基金Supported by the Natural Science Foundation of Heilongjiang Province of China(LH2021C038)the Earmarked Fund for CARS-35the National Key R&D Program of China(2018YFD0501202)。
文摘In order to obtain probiotics that can adapt to the intestinal environment of pigs and inhibit Escherichia coli(E.coli)K88,microorganisms were screened from the feces of Min pigs,and their antibacterial and biological characteristics were studied.Using E.coli K88 as the indicator bacterium,the supernatant of the isolated strain from Min pigs was co-cultured with the indicator bacteria for the antibacterial test.The Bacillus velezensis(B.velezensis)strain MZ-78 showed stronger antimicrobial activity against E.coli K88 than other isolates,and its supernatant inhibited the growth of other pathogens including Salmonella typhimurium(S.typhimurium),E.coli,Staphylococcus aureus(S.aureus),and Pseudomonas aeruginosa(P.aeruginosa).Further studies showed that the selected strain MZ-78 exhibited a certain tolerance to sodium chloride conditions.The B.velezensis strain MZ-78 exhibited no hemolytic activity and was not susceptible to antibiotics.Additionally,during metabolism,it generated a certain quantity of short-chain fatty acids,including isobutyric acid and isovaleric acid.Furthermore,this strain demonstrated the capability to counteract the adhesion of E.coli K88 to porcine small intestinal epithelial cells(IPEC-J2),while also promoting their growth and proliferation.The B.velezensis strain MZ-78 isolated from Min pigs feces showed a series of probiotic characteristics and the potential to survive in the digestive tract of animals,which indicated its potential commercial application value as a probiotic alternative to antibiotics in animal husbandry.
基金supported by the National Natural Science Foundation of China(NSFC)under grant No.12233012,the Strategic Priority Research Program of the Chinese Academy of Sciences,grant No.XDB0560102the National Key R&D Program of China 2022YFF0503003(2022YFF0503000)。
文摘The in-flight calibration and performance of the Solar Disk Imager(SDI),which is a pivotal instrument of the LyαSolar Telescope onboard the Advanced Space-based Solar Observatory mission,suggested a much lower spatial resolution than expected.In this paper,we developed the SDI point-spread function(PSF)and Image Bivariate Optimization Algorithm(SPIBOA)to improve the quality of SDI images.The bivariate optimization method smartly combines deep learning with optical system modeling.Despite the lack of information about the real image taken by SDI and the optical system function,this algorithm effectively estimates the PSF of the SDI imaging system directly from a large sample of observational data.We use the estimated PSF to conduct deconvolution correction to observed SDI images,and the resulting images show that the spatial resolution after correction has increased by a factor of more than three with respect to the observed ones.Meanwhile,our method also significantly reduces the inherent noise in the observed SDI images.The SPIBOA has now been successfully integrated into the routine SDI data processing,providing important support for the scientific studies based on the data.The development and application of SPIBOA also paves new ways to identify astronomical telescope systems and enhance observational image quality.Some essential factors and precautions in applying the SPIBOA method are also discussed.
文摘在“双碳”目标驱动下,综合能源系统(integrated energy system,IES)参与电-碳市场协同优化面临自愿减排激励不足与跨主体利益分配失衡的双重挑战,亟须构建兼顾经济性、低碳性与公平性的市场交易机制。提出一种综合能源系统自愿碳减排的电-碳市场投标竞价与利益分配机制。基于核密度估计与Wasserstein距离生成风光不确定性场景,考虑风电及光伏出力预测误差对电-碳市场投标的影响,建立了三阶段电-碳市场投标竞价与利益分配方法。第一阶段为日前电-碳预投标阶段,基于风光出力的预测值进行预投标,获得预期投标量与预计碳排放。第二阶段为日内调度阶段,考虑IES间的合作博弈与能源共享,确定模糊集内最坏情况分布中最小化调度成本。第三阶段为利益分配阶段,采用Myerson值与Shapley值分配方法分配收益,同时考虑碳转移、碳抵消、碳补偿等多种碳减排机制,激励IES减少系统碳排放,减少投标成本。最后以某工业园区为例,验证了自愿碳减排的电-碳市场投标竞价与利益分配方法的有效性。
基金supported by the Key Technologies R&D Program of China(2021YFA1502804)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0450102)+2 种基金the National Natural Science Foundation of China(22172150,22221003,22222306,22432004)the Innovation Program for Quantum Science and Technology(2021ZD0303302)high-performance computational resources provided by the University of Science and Technology of China and the Hefei Advanced Computing Center.
文摘Transition metal catalysts supported on oxides undergo significant structural changes under reaction conditions,which are influenced by interactions with reactants.These interactions can lead to the dispersion of metal atoms,leading to the formation of stable single-nucleus metal complexes.Understanding the stability of these complexes is essential for catalyst design.In this study,we use multitask symbolic regression to identify a descriptor for the stability of singlenucleus metal complexes with common reactants,such as CO and H_(2)O,on the basis of first-principles calculations.We develop a multidimensional descriptor incorporating metal‒metal,metal‒support,and metal‒adsorbate interactions,achieving high accuracy in predicting the stability of single-nucleus metal complexes.Our analysis revealed that in the absence of reactants,the stability of single metal atoms is mainly determined by the hardness(cohesive energy)of the metal.The presence of reactants such as CO and H_(2)O further stabilizes single-nucleus metal complexes by saturating undercoordinated metal atoms.This stabilization is correlated with the Lewis acidity of the surface oxygen in the support.Supports with lower Lewis acidity enhance metal‒support interactions,promoting CO adsorption on all metals and H_(2)O adsorption on complexes of hard metals.In contrast,supports with higher Lewis acidity enhance hydrogen adsorption,promoting H_(2)O interactions with complexes of soft metals as well as Pd and Pt.Additionally,our descriptor predicts nanoparticle(NP)dissociation into single-nucleus metal complexes or Ostwald ripening(OR)tendencies.Under CO conditions,harder metals tend to favor dissociation,whereas softer metals(e.g.,Ag,Cu,and Au)are more prone to OR.Under H_(2)O conditions,the Lewis acidity of the support surface oxygen influences NP behavior,with supports such as CeO_(2)stabilizing single-nucleus metal complexes and promoting NP dissociation.These insights provide guidance for selecting catalyst components and optimizing reaction conditions to control the stability of single-nucleus metal complexes and guide NP dissociation or OR processes.
基金partially supported by National Natural Science Foundation of China(52172250)Institute of Process Engineering(IPE)Project for Frontier Basic Research(QYJC-2023-06)。
文摘The use of lithium-sulfur(Li-S)batteries is limited by sulfur redox reactions involving multi-phase transformations,especially at low-temperatures.To address this issue,we report a material(FCNS@NCFs)consisting of nitrogen-doped carbon fibers loaded with a ternary metal sulf-ide((Fe,Co,Ni)_(9)S_(8))for use as the sulfur host in Li-S batteries.This materi-al was prepared using transfer blot filter paper as the carbon precursor,thiourea as the source of nitrogen and sulfur,and FeCl_(3)·6H_(2)O,CoCl_(2)·6H_(2)O and NiCl_(2)·6H_(2)O as the metal ion sources.It was synthesized by an impreg-nation method followed by calcination.The nitrogen doping significantly in-creased the conductivity of the host,and the metal sulfides have excellent catalytic activities.Theoretical calculations,and adsorption and deposition experiments show that active sites on the surface of FCNS@NCFs selectively adsorb polysulfides,facilitate rapid adsorption and conversion,prevent cathode passivation and inhib-it the polysulfide shuttling.The FCNS@NCFs used as the sulfur host has excellent electrochemical properties.Its initial dis-charge capacity is 1639.0 mAh g^(−1) at 0.2 C and room temperature,and it remains a capacity of 1255.1 mAh g^(−1) after 100 cycles.At−20~C,it has an initial discharge capacity of 1578.5 mAh g^(−1) at 0.2 C,with a capacity of 867.5 mAh g^(−1) after 100 cycles.Its excellent performance at both ambient and low temperatures suggests a new way to produce high-performance low-temper-ature Li-S batteries.
基金National Natural Science Foundation(9174110013) Aviation Power Fund(614B090310)
文摘To obtain the outlet temperature of combustor,a kind of high-temperature and water cooling thermocouple was designed.The main factors affecting the results of thermocouple measurement were analyzed after numerical simulation.Results showed that the high-temperature water cooling thermocouple can achieve high temperature measurement under the condition of 2 400 K.With the increase of the distance between the water cooling structure and the stagnation cover,the temperature measurement result was more accurate,and the increase in the area ratio of the inlet and outlet of the stagnation cover within a reasonable range can make the measurement results more accurate.The surface emissivity of the measuring point had a great influence on the radiation error.The pressure and flow rate of cooling water can be effectively reduced after adding zirconia coating onto the surface of the rake body.
文摘现有基于虚拟正弦信号注入(virtual sine signal injection,VSSI)的最大转矩电流比(maximum torque per ampere,MTPA)控制方法以理想永磁同步电动机(permanent magnet synchronous machine,PMSM)数学模型为理论基础,未考虑电感变化带来的不利影响。为了提升MTPA控制效果,增加考虑磁路交叉耦合效应,提出在线电感辨识方法和改进的MTPA控制方法。基于帕德逼近与变步长神经网络的电感辨识方法,不仅可以解决辨识模型欠秩问题,而且能够在线更新变化的电感参数,结合改进的基于VSSI的MTPA控制,可以有效降低电流矢量角误差,减小定子铜损耗,实现更准确的MTPA控制。通过仿真实验对所提方法的准确性进行了验证。
