目的对银花抗病毒口服液的化学成分进行分析鉴定。方法采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS)正负离子模式,Agilent ...目的对银花抗病毒口服液的化学成分进行分析鉴定。方法采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS)正负离子模式,Agilent ZORBAXEclipsePlusC_(18)(100mm×3.0mm,1.8μm)色谱柱;流动相为体积分数0.1%甲酸(A)和乙腈(B),梯度洗脱;流速为0.4mL·min^(-1);柱温为30℃;进样量为1μL。采用Agilent Masshunter Qualitative Analysis工作站中的分子特征提取(molecular feature extraction,MFE)功能,根据正负离子模式质谱扫描提供化合物一级、二级质谱数据的质谱碎片离子信息,采用中药成分数据库(traditional Chinese medicine component database,TCM-DATA),对照品比对等进行定性分析,并对化学成分进行归属。结果从银花口服液中共鉴定出85个化学成分,包括黄酮类20个、萜类19个、生物碱类5个、苯乙醇苷类6个、酚酸类10个、苯丙素类10个、其他类15个,并对各成分来源进行归属,同时对酚酸类、生物碱类、黄酮类、苯乙醇苷类化合物的裂解规律进行分析。结论建立的方法灵敏度高、准确高效,能够全面、快速地分析和鉴定银花抗病毒口服液的化学成分,为银花抗病毒口服液的质量控制和物质基础研究提供依据。展开更多
目的:利用数据挖掘方法,探析中医药治疗转移性乳腺癌(metastatic breast cancer,mBC)的用药规律及病机治法特点。方法:检索2000年1月至2021年9月数据库中有关中医药治疗mBC的文献,运用中医传承辅助系统平台(V2.5)对中药复方进行频次统...目的:利用数据挖掘方法,探析中医药治疗转移性乳腺癌(metastatic breast cancer,mBC)的用药规律及病机治法特点。方法:检索2000年1月至2021年9月数据库中有关中医药治疗mBC的文献,运用中医传承辅助系统平台(V2.5)对中药复方进行频次统计、关联规则和聚类分析,研究中医药治疗mBC用药规律并探讨其病机治法。结果:经筛选后,纳入108首中药复方,涉及210味中药。高频药物有白术、黄芪、茯苓、熟地黄、白花蛇舌草、山慈菇、白芍、党参、当归、莪术、补骨脂等,药类多为补虚药、活血化瘀药、利水渗湿药及清热药四大类,药性以甘温为主,归经以肝脾肾经为主。出现频次最高的药对是“白术-茯苓”。挖掘出6组核心药物配伍及3个新方组合。结论:mBC多属本虚标实之证,病机多为肝、脾、肾三脏失调,中医药治疗mBC以“扶正-活血-解毒”为主轴,辨证采用补气活血、补肾健脾、清热解毒为主线的治法,随证加减。展开更多
OBJECTIVE:To investigate the effects of luteolin on chronic unpredictable mild stress(CUMS)-induced depressive rats and corticosterone(CORT)-induced depressive primary hippocampal neurons,and to elucidate the mechanis...OBJECTIVE:To investigate the effects of luteolin on chronic unpredictable mild stress(CUMS)-induced depressive rats and corticosterone(CORT)-induced depressive primary hippocampal neurons,and to elucidate the mechanism behind the action.METHODS:The antidepressant mechanism of luteolin was studied by using CUMS rat model and primary hippocampal neurons in fetal rats.In vivo,novelty suppressed feeding,open-field and sucrose preference tests as well as Morris water maze were evaluated.The content of brain derived neurotrophic factor(BDNF),5-hydroxytryptamine(5-HT),norepinephrine(NE),and dopamine(DA)in serum were detected by enzyme-linked immunosorbent assay.The mechanisms of luteolin were explored based on neurotrophin and hippocampal neurogenesis,and proliferation.Survival of the septotemporal axis in hippocampus was assayed using the 5-bromo-2-deoxyuridine(BrdU),the expression of BDNF,neurotrophin-3(NT-3),and nerve growth factor(NGF)in hippocampus dentate gyrus region were measured by Western-blotting.In vitro,BDNF,NT-3,tropomyosin receptor kinase B(TrkB),and phosphorylated cyclic adenosine monophosphate responsive element binding protein(p-CREB)were detected through the high content analysis(HCA)to investigate neurotrophin and apoptosis.RESULTS:Induction of CUMS in rats induced depressive symptoms,while luteolin significantly enhanced sucrose consumption,decreased feeding latency,increased locomotor activity,escape latency,distance of target quadrant and regulated the content of depressive-like biomarkers.Histology analysis revealed that luteolin increased the abundance of new born neurons that had been labeled with BrdU,BrdU+neuronal nuclear antigen,and BrdU+doublecortin in septo-temporal axis of S2(mid-septal)and T3(mid-temporal).Moreover,expression of BDNF,NT-3,and NGF increased significantly in the septo-temporal axis of S2 and T3.HCA showed increased expression of BDNF,NT-3,TrkB and p-CREB in primary hippocampal neurons.CONCLUSION:The results provided direct evidence that luteolin has an antidepressant effect and could effectively promote the regeneration of the septotemporal axis nerve and hippocampal neuronutrition,which suggested that the antidepressant effect of luteolin may be related to hippocampal neurogenesis.展开更多
文摘目的对银花抗病毒口服液的化学成分进行分析鉴定。方法采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS)正负离子模式,Agilent ZORBAXEclipsePlusC_(18)(100mm×3.0mm,1.8μm)色谱柱;流动相为体积分数0.1%甲酸(A)和乙腈(B),梯度洗脱;流速为0.4mL·min^(-1);柱温为30℃;进样量为1μL。采用Agilent Masshunter Qualitative Analysis工作站中的分子特征提取(molecular feature extraction,MFE)功能,根据正负离子模式质谱扫描提供化合物一级、二级质谱数据的质谱碎片离子信息,采用中药成分数据库(traditional Chinese medicine component database,TCM-DATA),对照品比对等进行定性分析,并对化学成分进行归属。结果从银花口服液中共鉴定出85个化学成分,包括黄酮类20个、萜类19个、生物碱类5个、苯乙醇苷类6个、酚酸类10个、苯丙素类10个、其他类15个,并对各成分来源进行归属,同时对酚酸类、生物碱类、黄酮类、苯乙醇苷类化合物的裂解规律进行分析。结论建立的方法灵敏度高、准确高效,能够全面、快速地分析和鉴定银花抗病毒口服液的化学成分,为银花抗病毒口服液的质量控制和物质基础研究提供依据。
文摘目的:利用数据挖掘方法,探析中医药治疗转移性乳腺癌(metastatic breast cancer,mBC)的用药规律及病机治法特点。方法:检索2000年1月至2021年9月数据库中有关中医药治疗mBC的文献,运用中医传承辅助系统平台(V2.5)对中药复方进行频次统计、关联规则和聚类分析,研究中医药治疗mBC用药规律并探讨其病机治法。结果:经筛选后,纳入108首中药复方,涉及210味中药。高频药物有白术、黄芪、茯苓、熟地黄、白花蛇舌草、山慈菇、白芍、党参、当归、莪术、补骨脂等,药类多为补虚药、活血化瘀药、利水渗湿药及清热药四大类,药性以甘温为主,归经以肝脾肾经为主。出现频次最高的药对是“白术-茯苓”。挖掘出6组核心药物配伍及3个新方组合。结论:mBC多属本虚标实之证,病机多为肝、脾、肾三脏失调,中医药治疗mBC以“扶正-活血-解毒”为主轴,辨证采用补气活血、补肾健脾、清热解毒为主线的治法,随证加减。
基金Outstanding Youth Project of Natural Science Foundation of Hunan Province:Mechanisms of Emotional-behavioural and Cognitive Impairment in Depression and Intervention with Traditional Chinese Medicine(No.2020JJ3027)the Training Plan of Outstanding Innovative Youth of Changsha(No.kq2009018)+2 种基金Key Projects of Hunan Provincial Department of Education:Hspa5/Pr Pcbased Study on the Mechanism of Lymphoid System Damage in Cerebral Small Vessel Disease with Depression and Intervention with Traditional Chinese Medicine(23A0281)Traditional Chinese Medicine Research Program Project of Hunan Province:Mechanisms of Impairment of Ventral and Dorsal Hippocampal Differential Function in Depression based on Sonic Hedgehog/Brain-Derived Neurotrophic Factor Signalling-Mediated Synaptogenesis in Newborn Neurons and Intervention with Traditional Chinese Medicine(B2023021)Open Fund Project of State Key Laboratory of Traditional Chinese Medicine Powder and Innovative Drugs:Mechanisms of Glymphatic Iinjury in Cerebral Small Vessel Disease with Depression Based on Aquaporin Protein-4 Signalling-Mediated Astrocyte-Responsive Hyperplasia and Intervention with Traditional Chinese Medicine(23PTKF1013)。
文摘OBJECTIVE:To investigate the effects of luteolin on chronic unpredictable mild stress(CUMS)-induced depressive rats and corticosterone(CORT)-induced depressive primary hippocampal neurons,and to elucidate the mechanism behind the action.METHODS:The antidepressant mechanism of luteolin was studied by using CUMS rat model and primary hippocampal neurons in fetal rats.In vivo,novelty suppressed feeding,open-field and sucrose preference tests as well as Morris water maze were evaluated.The content of brain derived neurotrophic factor(BDNF),5-hydroxytryptamine(5-HT),norepinephrine(NE),and dopamine(DA)in serum were detected by enzyme-linked immunosorbent assay.The mechanisms of luteolin were explored based on neurotrophin and hippocampal neurogenesis,and proliferation.Survival of the septotemporal axis in hippocampus was assayed using the 5-bromo-2-deoxyuridine(BrdU),the expression of BDNF,neurotrophin-3(NT-3),and nerve growth factor(NGF)in hippocampus dentate gyrus region were measured by Western-blotting.In vitro,BDNF,NT-3,tropomyosin receptor kinase B(TrkB),and phosphorylated cyclic adenosine monophosphate responsive element binding protein(p-CREB)were detected through the high content analysis(HCA)to investigate neurotrophin and apoptosis.RESULTS:Induction of CUMS in rats induced depressive symptoms,while luteolin significantly enhanced sucrose consumption,decreased feeding latency,increased locomotor activity,escape latency,distance of target quadrant and regulated the content of depressive-like biomarkers.Histology analysis revealed that luteolin increased the abundance of new born neurons that had been labeled with BrdU,BrdU+neuronal nuclear antigen,and BrdU+doublecortin in septo-temporal axis of S2(mid-septal)and T3(mid-temporal).Moreover,expression of BDNF,NT-3,and NGF increased significantly in the septo-temporal axis of S2 and T3.HCA showed increased expression of BDNF,NT-3,TrkB and p-CREB in primary hippocampal neurons.CONCLUSION:The results provided direct evidence that luteolin has an antidepressant effect and could effectively promote the regeneration of the septotemporal axis nerve and hippocampal neuronutrition,which suggested that the antidepressant effect of luteolin may be related to hippocampal neurogenesis.
