目的基于文献研究归纳新外来中药海葱的中药药性,应用智能感官进一步验证并探讨其五味药性,为新外来中药的药性研究提供思路。方法通过中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(Wanfang Data)、PubMed、We...目的基于文献研究归纳新外来中药海葱的中药药性,应用智能感官进一步验证并探讨其五味药性,为新外来中药的药性研究提供思路。方法通过中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(Wanfang Data)、PubMed、Web of Science检索海葱相关研究文献,结合中医理论归纳海葱的中药药性。应用PEN3型电子鼻和SA402B型电子舌采集海葱的智能感官信息,利用主成分分析法辨识海葱的五味药性。结果通过文献研究归纳得到海葱具有抗氧化、抗肿瘤、强心利尿、止咳平喘等药理作用,结合理论探讨可知,海葱性微温,味苦、辛、甘,有小毒,归肺、脾、胃、心经。基于智能感官的海葱五味药性辨识结果为苦、微辛、微甘、咸、非酸,与文献研究结果基本一致。结论通过文献研究结合智能感官可以较好地归纳和辨识海葱的中药药性,本研究可为客观、充分地探析新外来中药的药性提供参考。展开更多
以蓝圆鲹(Decapterus maruadsi)分离蛋白为原料,采用碱性蛋白酶对其进行限制性酶解,研究水解度(degree of hydrolysis,DH)对分离蛋白酶解产物溶解性、持油力、乳化性与起泡性等功能特性的影响。结果表明,碱性蛋白酶酶解产物的相对分子...以蓝圆鲹(Decapterus maruadsi)分离蛋白为原料,采用碱性蛋白酶对其进行限制性酶解,研究水解度(degree of hydrolysis,DH)对分离蛋白酶解产物溶解性、持油力、乳化性与起泡性等功能特性的影响。结果表明,碱性蛋白酶酶解产物的相对分子质量显著下降。酶解可有效提高分离蛋白的溶解性,其溶解度随DH增加而增加。与对照组相比,分离蛋白经酶解后,产物乳化性与起泡性均显著提高,并呈现先升高后降低的趋势。不同DH酶解产物在pH值为4.0时的乳化性和起泡性最低;当pH值为10.0时,DH5(DH=5%)的乳化性最高((100.9±0.7)m2/g);当pH值为7.0时,DH5起泡性最高((227.3±3.8)%)。除DH20外,其他组的持油力均有显著提高,其中DH5的持油力最高,达3.50 g/g(油/蛋白)。结果表明,一定程度的水解可以显著提高蓝圆鲹分离蛋白的功能特性。本研究为鱼蛋白在食品蛋白配料中的应用提供了一定理论参考。展开更多
以蓝圆鲹(Decapterus maruadsi)为研究对象,采用酸/碱溶解-等电点沉淀法制备蓝圆鲹肌肉分离蛋白(acid/alkaline aided protein isolate,API/KPI),分析比较不同分离蛋白与肌肉全蛋白(total protein,TP)的自身降解规律,并对其水溶性蛋白...以蓝圆鲹(Decapterus maruadsi)为研究对象,采用酸/碱溶解-等电点沉淀法制备蓝圆鲹肌肉分离蛋白(acid/alkaline aided protein isolate,API/KPI),分析比较不同分离蛋白与肌肉全蛋白(total protein,TP)的自身降解规律,并对其水溶性蛋白酶的酶学性质展开研究。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)结果显示,TP与KPI均会发生强烈的自身降解现象,API则无明显的自身降解。酶谱与酶活力测定结果表明,KPI保留了TP超过80%的酶活性,而API则仅剩10%的水解活性。酶学性质结果表明,TP的内源酶具有两个最适pH值,分别为3.0与9.0,而KPI的最适pH值为9.0;TP与KPI内源酶的最适温度均为60℃。荧光底物结果表明,TP与KPI的最适荧光底物为Boc-Gln-Arg-Arg-MCA,且对羧基端为Arg的底物均具有较高的水解活性。抑制剂结果显示,TP和KPI水溶性蛋白酶都能被丝氨酸蛋白酶抑制剂强烈抑制,暗示水溶性丝氨酸蛋白酶在碱法等电点制备的分离蛋白凝胶劣化中起关键作用。展开更多
The housing price has been paid close attention by people in all walks of life,and the development of big data provides a new data environment for the study of urban housing price.Housing price data of four national c...The housing price has been paid close attention by people in all walks of life,and the development of big data provides a new data environment for the study of urban housing price.Housing price data of four national central cities (Beijing,Shanghai,Guangzhou and Wuhan) are taken as research samples.With the help of software GIS,exploratory spatial data analysis method is used to depict the spatial distribution pattern of urban housing price,and commonness and difference of spatial distribution of housing price are explored.The conclusions are as below:①regional imbalance of housing price in national central cities is significant.②Spatial distribution of urban housing price in Beijing,Shanghai,Guangzhou and Wuhan presents a polycentric pattern,and there is obvious spatial agglomeration.③The internal change of housing price in different cities has significant spatial difference.Beijing,Shanghai,Guangzhou and Wuhan are taken as typical city samples for research,with reference and practical significance,which could help to effectively predict spatial development trend of housing prices in other first and second tier cities.The research aims to provide certain reference for the government implementing real estate control policies according to local conditions,project location and reasonable pricing of real estate developers.展开更多
In recent years,more and more researches focus on the self characteristics and spatial location of housing,and explore the influencing factors of urban housing price from the micro perspective.As representative of big...In recent years,more and more researches focus on the self characteristics and spatial location of housing,and explore the influencing factors of urban housing price from the micro perspective.As representative of big cities,spatial distribution pattern of housing price in national central cities has attracted much attention.In order to return the spatial distribution pattern of housing price to the research on influencing factors of housing price,the reasons behind the spatial distribution pattern of housing price in three national central cities:Beijing,Wuhan and Chongqing are explored.The results show that①urban housing price is affected by many factors.Due to different social and economic conditions in each city,there are differences in the influence direction of the proximity to expressways,city squares,universities and living facilities,characteristics of companies and enterprises on Beijing,Wuhan and Chongqing.②Various factors have different value-added effects on housing price in different cities.The location of ring line in Beijing and Wuhan has the greatest increase effect on housing price,while metro station of Chongqing has the greatest increase effect on housing price.展开更多
畸形性骨炎于1876年由James Paget首先报道,因此又称为Paget′s骨病(Paget′s disease of bone,PDB)^([1])。其临床特征是骨痛、骨骼畸形和骨折。该病发病率存在明显地域差异,与种族及年龄也有很大关系,男性较女性更易罹患,多见于55岁...畸形性骨炎于1876年由James Paget首先报道,因此又称为Paget′s骨病(Paget′s disease of bone,PDB)^([1])。其临床特征是骨痛、骨骼畸形和骨折。该病发病率存在明显地域差异,与种族及年龄也有很大关系,男性较女性更易罹患,多见于55岁以上人群^([2])。由于国内该病较罕见,常被延误诊治。展开更多
文摘目的基于文献研究归纳新外来中药海葱的中药药性,应用智能感官进一步验证并探讨其五味药性,为新外来中药的药性研究提供思路。方法通过中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(Wanfang Data)、PubMed、Web of Science检索海葱相关研究文献,结合中医理论归纳海葱的中药药性。应用PEN3型电子鼻和SA402B型电子舌采集海葱的智能感官信息,利用主成分分析法辨识海葱的五味药性。结果通过文献研究归纳得到海葱具有抗氧化、抗肿瘤、强心利尿、止咳平喘等药理作用,结合理论探讨可知,海葱性微温,味苦、辛、甘,有小毒,归肺、脾、胃、心经。基于智能感官的海葱五味药性辨识结果为苦、微辛、微甘、咸、非酸,与文献研究结果基本一致。结论通过文献研究结合智能感官可以较好地归纳和辨识海葱的中药药性,本研究可为客观、充分地探析新外来中药的药性提供参考。
文摘以蓝圆鲹(Decapterus maruadsi)分离蛋白为原料,采用碱性蛋白酶对其进行限制性酶解,研究水解度(degree of hydrolysis,DH)对分离蛋白酶解产物溶解性、持油力、乳化性与起泡性等功能特性的影响。结果表明,碱性蛋白酶酶解产物的相对分子质量显著下降。酶解可有效提高分离蛋白的溶解性,其溶解度随DH增加而增加。与对照组相比,分离蛋白经酶解后,产物乳化性与起泡性均显著提高,并呈现先升高后降低的趋势。不同DH酶解产物在pH值为4.0时的乳化性和起泡性最低;当pH值为10.0时,DH5(DH=5%)的乳化性最高((100.9±0.7)m2/g);当pH值为7.0时,DH5起泡性最高((227.3±3.8)%)。除DH20外,其他组的持油力均有显著提高,其中DH5的持油力最高,达3.50 g/g(油/蛋白)。结果表明,一定程度的水解可以显著提高蓝圆鲹分离蛋白的功能特性。本研究为鱼蛋白在食品蛋白配料中的应用提供了一定理论参考。
文摘以蓝圆鲹(Decapterus maruadsi)为研究对象,采用酸/碱溶解-等电点沉淀法制备蓝圆鲹肌肉分离蛋白(acid/alkaline aided protein isolate,API/KPI),分析比较不同分离蛋白与肌肉全蛋白(total protein,TP)的自身降解规律,并对其水溶性蛋白酶的酶学性质展开研究。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)结果显示,TP与KPI均会发生强烈的自身降解现象,API则无明显的自身降解。酶谱与酶活力测定结果表明,KPI保留了TP超过80%的酶活性,而API则仅剩10%的水解活性。酶学性质结果表明,TP的内源酶具有两个最适pH值,分别为3.0与9.0,而KPI的最适pH值为9.0;TP与KPI内源酶的最适温度均为60℃。荧光底物结果表明,TP与KPI的最适荧光底物为Boc-Gln-Arg-Arg-MCA,且对羧基端为Arg的底物均具有较高的水解活性。抑制剂结果显示,TP和KPI水溶性蛋白酶都能被丝氨酸蛋白酶抑制剂强烈抑制,暗示水溶性丝氨酸蛋白酶在碱法等电点制备的分离蛋白凝胶劣化中起关键作用。
基金Sponsored by National Natural Science Foundation of China (51808413)General Project of Hubei Social Science Fund (2018193)Innovation and Entrepreneurship Training Program for College Students in Hubei Province (S201910490027)。
文摘The housing price has been paid close attention by people in all walks of life,and the development of big data provides a new data environment for the study of urban housing price.Housing price data of four national central cities (Beijing,Shanghai,Guangzhou and Wuhan) are taken as research samples.With the help of software GIS,exploratory spatial data analysis method is used to depict the spatial distribution pattern of urban housing price,and commonness and difference of spatial distribution of housing price are explored.The conclusions are as below:①regional imbalance of housing price in national central cities is significant.②Spatial distribution of urban housing price in Beijing,Shanghai,Guangzhou and Wuhan presents a polycentric pattern,and there is obvious spatial agglomeration.③The internal change of housing price in different cities has significant spatial difference.Beijing,Shanghai,Guangzhou and Wuhan are taken as typical city samples for research,with reference and practical significance,which could help to effectively predict spatial development trend of housing prices in other first and second tier cities.The research aims to provide certain reference for the government implementing real estate control policies according to local conditions,project location and reasonable pricing of real estate developers.
基金Sponsored by National Natural Science Foundation of China (51808413)General Project of Hubei Social Science Fund (2018193)+1 种基金Innovation and Entrepreneurship Training Program for College Students in Hubei Province (S201910490024)University-level Graduate Innovation Fund of Wuhan Institute of Technology (CX2019036)。
文摘In recent years,more and more researches focus on the self characteristics and spatial location of housing,and explore the influencing factors of urban housing price from the micro perspective.As representative of big cities,spatial distribution pattern of housing price in national central cities has attracted much attention.In order to return the spatial distribution pattern of housing price to the research on influencing factors of housing price,the reasons behind the spatial distribution pattern of housing price in three national central cities:Beijing,Wuhan and Chongqing are explored.The results show that①urban housing price is affected by many factors.Due to different social and economic conditions in each city,there are differences in the influence direction of the proximity to expressways,city squares,universities and living facilities,characteristics of companies and enterprises on Beijing,Wuhan and Chongqing.②Various factors have different value-added effects on housing price in different cities.The location of ring line in Beijing and Wuhan has the greatest increase effect on housing price,while metro station of Chongqing has the greatest increase effect on housing price.
文摘畸形性骨炎于1876年由James Paget首先报道,因此又称为Paget′s骨病(Paget′s disease of bone,PDB)^([1])。其临床特征是骨痛、骨骼畸形和骨折。该病发病率存在明显地域差异,与种族及年龄也有很大关系,男性较女性更易罹患,多见于55岁以上人群^([2])。由于国内该病较罕见,常被延误诊治。
