The use of antifouling agents is suggested to be a promising method for protecting oceanic instruments from biological contamination.We developed a novel antifouling material doped with capsaicin(CAP)as a filler and m...The use of antifouling agents is suggested to be a promising method for protecting oceanic instruments from biological contamination.We developed a novel antifouling material doped with capsaicin(CAP)as a filler and montmorillonite(MMT)as a carrier for the practical application of CTD(conductivity,temperature,depth)protection.The optimal parameters for preparing the material were established,and the obtained material achieved the maximum CAP loading capacity of 32.74%.The proposed material exhibited great release properties in acidic environments,which is beneficial for reducing bacterial attachment.Furthermore,the optimal conditions(temperature,flow rate,and pressure in the aquatic environment)for a better release rate of the material were determined through a series of simulation tests in lab.It provided good guidance and basis for practical application of the material.The CAP@MMT composite showed excellent efficiency and effectiveness in preventing the attachment of microorganisms during the four-month marine field tests.In the subsequent experiments,the great properties of the antifouling material were further confirmed by retesting the conductivity of four instruments participating in marine field tests.The measuring errors of CTD protected by the antifouling material are both within 0.01 mS/cm,which is far lower than that of the other two instruments.展开更多
The Lorentz force generated by electromagnetic field on the surface of the cylinder in the electrolyte solution may modify the structure of the flow boundary layer effectively. The transient control process of Lorentz...The Lorentz force generated by electromagnetic field on the surface of the cylinder in the electrolyte solution may modify the structure of the flow boundary layer effectively. The transient control process of Lorentz force is investigated experimentally for lift amplification and vibration suppression. The experiments are conducted in a rotating annular tank filled with a low-conducting electrolyte. A cylinder with an electro-magnetic actuator is placed into the electrolyte. The lift force of cylinder is measured using the strain gages attached to a fixed beam, and the flow fields are visualized by the dye markers. The results show that the upper vortex on the cylinder is suppressed, and the wake becomes a line and leans to the lower side under the action of upside Lorentz force while the lower vortex on the cylinder is suppressed and limited in a small region. Therefore, the value of lift increases with the variation of flow field. However, the vortexes on the cylinder are suppressed fully under the action of symmetrical Lorentz force which leads to the suppression of lift oscillation and then the vibration of cylinder are suppressed fully.展开更多
Inductively coupled mooring chain transmission technology plays a crucial role in the long-distance online monitoring of marine hydrographic information.However,the impedance characteristics of the seawater medium pos...Inductively coupled mooring chain transmission technology plays a crucial role in the long-distance online monitoring of marine hydrographic information.However,the impedance characteristics of the seawater medium pose a limitation on its capability to achieve high-speed real-time transmission of underwater images.In this paper,based on the principle of inductively coupled transmission for marine applications,the selected 1920×1080 pixel images are segmented,packaged,and then transmitted to the sending node using the user datagram protocol(UDP),and the modulation and demodulation of the transmitted images are realized using the orthogonal frequency division multiplexing(OFDM)algorithm of least squares(LS)estimation,and eight combinations with different mapping modes and coding rates are tested in the bandwidth of 200-800 k Hz and the quality of the transmitted images is evaluated using three criteria:mean squared error(MSE),peak signal to noise ratio(PSNR),and structural similarity index(SSIM).The results indicate that the image transmission quality is optimal when the mapping method is 16-quadrature amplitude modulation(16QAM),the coding rate is 1/2,and the center frequency is 800 k Hz.Under these conditions,the maximum transmission rate is 0.84 Mbit s^(-1),the SSIM criterion exceeds 0.91690 d B,and the learned perceptual image patch similarity(LPIPS)is less than 0.06000.This paper provides a solution for the underwater image transmission of inductively coupled mooring chains for marine applications.展开更多
In this study,a mathematical model of multipath channels is established,and the delay parameters of 10-path models are calculated at 300 m.A multipath-channel hardware simulator based on a field programmable gate arra...In this study,a mathematical model of multipath channels is established,and the delay parameters of 10-path models are calculated at 300 m.A multipath-channel hardware simulator based on a field programmable gate array(FPGA)is designed and verified at 100 k Hz,200 k Hz,500 k Hz,1 MHz,and 24 MHz transmission frequencies.According to the characteristics of the ocean induction coupling chain channel,the orthogonal frequency-division multiplexing(OFDM)algorithm parameters are designed by referring to the wireless communication protocol.The appropriate length cyclic prefix(CP)is added in the OFDM symbol to resist the multipath effect of the seawater channel,and the FPGA hardware transceiver based on the OFDM algorithm is realized.The hardware platform of the ocean induction coupling chain communication system is developed to resist the multipath effect of the seawater channel and tested at 24 MHz.The experimental results show that 800 ns is the best CP length for the developed system,which can effectively resist the multipath effect,with a signal-to-noise ratio above 24 d B and a bit error rate below 1%.This study provides a hardware simulation test platform and an effective method to resist the multipath effect of a seawater channel and improve the transmission performance of the seawater channel.展开更多
[目的]探究miR-325-3p靶向PRDX4对肾细胞癌细胞增殖、侵袭及凋亡的影响。[方法]设肾细胞癌细胞Caki-1组、miR-NC组、miR-325-3p-mimics组(过表达)、miR-325-3p-inhibitor组(低表达),测定各组细胞增殖、单克隆形成数目、凋亡率、侵袭水...[目的]探究miR-325-3p靶向PRDX4对肾细胞癌细胞增殖、侵袭及凋亡的影响。[方法]设肾细胞癌细胞Caki-1组、miR-NC组、miR-325-3p-mimics组(过表达)、miR-325-3p-inhibitor组(低表达),测定各组细胞增殖、单克隆形成数目、凋亡率、侵袭水平以及miR-325-3p、PRDX4水平。[结果]miR-325-3p-inhibitor组OD值(0.93±0.03)、存活率(86.58±6.36)%、单克隆形成数目(1062.29±102.78)、穿膜数(1917.34±425.35)、PRDX4 mRNA和蛋白表达水平(4.63±0.28、1.82±0.18)高于miR-325-3p-mimics组[(0.42±0.02)、(42.25±7.20)%、(239.89±35.27)个、(293.85±95.28)个、(2.04±0.24)、(0.38±0.07)](P<0.05),细胞凋亡率(1.12±0.29)%、miR-325-3p表达水平(1.42±0.38)低于miR-325-3p-mimics组(7.14±1.11)%、(5.68±0.37)(P<0.05)。[结论]miR-325-3p上调可以抑制肾细胞癌细胞的增殖(76.59%±7.30%vs 42.25%±7.20%)、迁移侵袭(702.28±111.52 vs 293.85±95.28),同时诱导细胞凋亡(3.46±1.04 vs 7.14±1.11),而这些过程主要是通过miR-325-3p与PRDX4的相互作用实现的。展开更多
To analyze the influence of _＋400 kV Q^nghai-Tibet HVDC transmission system on transmission- line protections in Qjnghai AG power system, a closed-loop simulation system was constructed by combing HyperSim system wit...To analyze the influence of _＋400 kV Q^nghai-Tibet HVDC transmission system on transmission- line protections in Qjnghai AG power system, a closed-loop simulation system was constructed by combing HyperSim system with HVDG control protection devices. Various faults on double-circuit 750 kV and multi- circuit 330 kV AC transmission lines in Qjnghai power system were simulated. The impedance characteristics and harmonic components at Qjnghai side of Qjng-Tibet DG transmission line were analyzed. The harmonic proportion in voltages and currents were studied for faults that took place at different locations near the DG system. The inflence of Qing-Tibet DG system on the directional components of protections, differential protections and distance protections of AC transmission lines was discussed and drew the conclusions that the DC sytem had little influence on differential protections, while had great inflence on directional components and distance protection. The conclusions can provide reference for studying the interaction between AC and DG systems.展开更多
[目的]探讨肾细胞癌中TXNDC5的表达及其对786-O细胞增殖、侵袭和迁移的影响。[方法]2020年8月-2023年8月102例肾细胞癌患者纳入研究,免疫组化染色测定TXNDC5表达水平,χ^(2)检验分析TXNDC5表达与肾细胞癌临床病理特征的关系。同时设肾...[目的]探讨肾细胞癌中TXNDC5的表达及其对786-O细胞增殖、侵袭和迁移的影响。[方法]2020年8月-2023年8月102例肾细胞癌患者纳入研究,免疫组化染色测定TXNDC5表达水平,χ^(2)检验分析TXNDC5表达与肾细胞癌临床病理特征的关系。同时设肾细胞癌细胞786-O组、sh-NC组、TXNDC5-inhibitor组、TXNDC5-mimics组,测定各组细胞增殖、侵袭、凋亡以及TXNDC5、CCN2水平。[结果]肾细胞癌组TXNDC5蛋白表达阳性率高于癌旁组(P<0.05)。TXNDC5蛋白表达与肾细胞癌临床病理特征相关。TXNDC5-inhibitor组OD值(0.78±0.05 vs 0.41±0.03)、存活率(78.56%±6.18%vs 42.11%±7.24%)、单克隆形成数目[(619.33±89.20)/个]、穿膜数[(323.85±84.25 vs 93.85±95.34)/个]、TXNDC5、CCN2 mRNA和蛋白表达水平低于肾细胞癌细胞786-O组、sh-NC组,凋亡率高于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于肾细胞癌细胞786-O组、sh-NC组,凋亡率低于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于TXNDC5-inhibitor组,凋亡率低于TXNDC5-inhibitor组(P<0.05)。[结论]TXNDC5下调可以抑制肾细胞癌细胞的增殖、迁移和侵袭,同时诱导细胞凋亡,这一过程与调节CCN2相关。展开更多
基金supported by the directional Foundation of the Key Laboratory of Ocean Observation Technology,MNR(No.2021KlootB06)the National Natural Science Foundation of China(No.52271341)。
文摘The use of antifouling agents is suggested to be a promising method for protecting oceanic instruments from biological contamination.We developed a novel antifouling material doped with capsaicin(CAP)as a filler and montmorillonite(MMT)as a carrier for the practical application of CTD(conductivity,temperature,depth)protection.The optimal parameters for preparing the material were established,and the obtained material achieved the maximum CAP loading capacity of 32.74%.The proposed material exhibited great release properties in acidic environments,which is beneficial for reducing bacterial attachment.Furthermore,the optimal conditions(temperature,flow rate,and pressure in the aquatic environment)for a better release rate of the material were determined through a series of simulation tests in lab.It provided good guidance and basis for practical application of the material.The CAP@MMT composite showed excellent efficiency and effectiveness in preventing the attachment of microorganisms during the four-month marine field tests.In the subsequent experiments,the great properties of the antifouling material were further confirmed by retesting the conductivity of four instruments participating in marine field tests.The measuring errors of CTD protected by the antifouling material are both within 0.01 mS/cm,which is far lower than that of the other two instruments.
基金Sponsored by the National Nature Science Foundation of China ( 11202102)pecialized Research Fund for Doctoral Program of High Educatio n ( 20123219120050)
文摘The Lorentz force generated by electromagnetic field on the surface of the cylinder in the electrolyte solution may modify the structure of the flow boundary layer effectively. The transient control process of Lorentz force is investigated experimentally for lift amplification and vibration suppression. The experiments are conducted in a rotating annular tank filled with a low-conducting electrolyte. A cylinder with an electro-magnetic actuator is placed into the electrolyte. The lift force of cylinder is measured using the strain gages attached to a fixed beam, and the flow fields are visualized by the dye markers. The results show that the upper vortex on the cylinder is suppressed, and the wake becomes a line and leans to the lower side under the action of upside Lorentz force while the lower vortex on the cylinder is suppressed and limited in a small region. Therefore, the value of lift increases with the variation of flow field. However, the vortexes on the cylinder are suppressed fully under the action of symmetrical Lorentz force which leads to the suppression of lift oscillation and then the vibration of cylinder are suppressed fully.
基金supported by grants from the National Natural Science Foundation of China(No.62071329)the National Science Foundation of Tianjin(No.23JCZD JC00440)。
文摘Inductively coupled mooring chain transmission technology plays a crucial role in the long-distance online monitoring of marine hydrographic information.However,the impedance characteristics of the seawater medium pose a limitation on its capability to achieve high-speed real-time transmission of underwater images.In this paper,based on the principle of inductively coupled transmission for marine applications,the selected 1920×1080 pixel images are segmented,packaged,and then transmitted to the sending node using the user datagram protocol(UDP),and the modulation and demodulation of the transmitted images are realized using the orthogonal frequency division multiplexing(OFDM)algorithm of least squares(LS)estimation,and eight combinations with different mapping modes and coding rates are tested in the bandwidth of 200-800 k Hz and the quality of the transmitted images is evaluated using three criteria:mean squared error(MSE),peak signal to noise ratio(PSNR),and structural similarity index(SSIM).The results indicate that the image transmission quality is optimal when the mapping method is 16-quadrature amplitude modulation(16QAM),the coding rate is 1/2,and the center frequency is 800 k Hz.Under these conditions,the maximum transmission rate is 0.84 Mbit s^(-1),the SSIM criterion exceeds 0.91690 d B,and the learned perceptual image patch similarity(LPIPS)is less than 0.06000.This paper provides a solution for the underwater image transmission of inductively coupled mooring chains for marine applications.
基金supported by the National Key Research and Development Program of China(Nos.2017YFC1403403,2017YFC1403304)。
文摘In this study,a mathematical model of multipath channels is established,and the delay parameters of 10-path models are calculated at 300 m.A multipath-channel hardware simulator based on a field programmable gate array(FPGA)is designed and verified at 100 k Hz,200 k Hz,500 k Hz,1 MHz,and 24 MHz transmission frequencies.According to the characteristics of the ocean induction coupling chain channel,the orthogonal frequency-division multiplexing(OFDM)algorithm parameters are designed by referring to the wireless communication protocol.The appropriate length cyclic prefix(CP)is added in the OFDM symbol to resist the multipath effect of the seawater channel,and the FPGA hardware transceiver based on the OFDM algorithm is realized.The hardware platform of the ocean induction coupling chain communication system is developed to resist the multipath effect of the seawater channel and tested at 24 MHz.The experimental results show that 800 ns is the best CP length for the developed system,which can effectively resist the multipath effect,with a signal-to-noise ratio above 24 d B and a bit error rate below 1%.This study provides a hardware simulation test platform and an effective method to resist the multipath effect of a seawater channel and improve the transmission performance of the seawater channel.
文摘[目的]探究miR-325-3p靶向PRDX4对肾细胞癌细胞增殖、侵袭及凋亡的影响。[方法]设肾细胞癌细胞Caki-1组、miR-NC组、miR-325-3p-mimics组(过表达)、miR-325-3p-inhibitor组(低表达),测定各组细胞增殖、单克隆形成数目、凋亡率、侵袭水平以及miR-325-3p、PRDX4水平。[结果]miR-325-3p-inhibitor组OD值(0.93±0.03)、存活率(86.58±6.36)%、单克隆形成数目(1062.29±102.78)、穿膜数(1917.34±425.35)、PRDX4 mRNA和蛋白表达水平(4.63±0.28、1.82±0.18)高于miR-325-3p-mimics组[(0.42±0.02)、(42.25±7.20)%、(239.89±35.27)个、(293.85±95.28)个、(2.04±0.24)、(0.38±0.07)](P<0.05),细胞凋亡率(1.12±0.29)%、miR-325-3p表达水平(1.42±0.38)低于miR-325-3p-mimics组(7.14±1.11)%、(5.68±0.37)(P<0.05)。[结论]miR-325-3p上调可以抑制肾细胞癌细胞的增殖(76.59%±7.30%vs 42.25%±7.20%)、迁移侵袭(702.28±111.52 vs 293.85±95.28),同时诱导细胞凋亡(3.46±1.04 vs 7.14±1.11),而这些过程主要是通过miR-325-3p与PRDX4的相互作用实现的。
文摘To analyze the influence of _＋400 kV Q^nghai-Tibet HVDC transmission system on transmission- line protections in Qjnghai AG power system, a closed-loop simulation system was constructed by combing HyperSim system with HVDG control protection devices. Various faults on double-circuit 750 kV and multi- circuit 330 kV AC transmission lines in Qjnghai power system were simulated. The impedance characteristics and harmonic components at Qjnghai side of Qjng-Tibet DG transmission line were analyzed. The harmonic proportion in voltages and currents were studied for faults that took place at different locations near the DG system. The inflence of Qing-Tibet DG system on the directional components of protections, differential protections and distance protections of AC transmission lines was discussed and drew the conclusions that the DC sytem had little influence on differential protections, while had great inflence on directional components and distance protection. The conclusions can provide reference for studying the interaction between AC and DG systems.
文摘[目的]探讨肾细胞癌中TXNDC5的表达及其对786-O细胞增殖、侵袭和迁移的影响。[方法]2020年8月-2023年8月102例肾细胞癌患者纳入研究,免疫组化染色测定TXNDC5表达水平,χ^(2)检验分析TXNDC5表达与肾细胞癌临床病理特征的关系。同时设肾细胞癌细胞786-O组、sh-NC组、TXNDC5-inhibitor组、TXNDC5-mimics组,测定各组细胞增殖、侵袭、凋亡以及TXNDC5、CCN2水平。[结果]肾细胞癌组TXNDC5蛋白表达阳性率高于癌旁组(P<0.05)。TXNDC5蛋白表达与肾细胞癌临床病理特征相关。TXNDC5-inhibitor组OD值(0.78±0.05 vs 0.41±0.03)、存活率(78.56%±6.18%vs 42.11%±7.24%)、单克隆形成数目[(619.33±89.20)/个]、穿膜数[(323.85±84.25 vs 93.85±95.34)/个]、TXNDC5、CCN2 mRNA和蛋白表达水平低于肾细胞癌细胞786-O组、sh-NC组,凋亡率高于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于肾细胞癌细胞786-O组、sh-NC组,凋亡率低于肾细胞癌细胞786-O组、sh-NC组(P<0.05);TXNDC5-mimics组OD值、存活率、单克隆形成数目、穿膜数、TXNDC5、CCN2 mRNA和蛋白表达水平高于TXNDC5-inhibitor组,凋亡率低于TXNDC5-inhibitor组(P<0.05)。[结论]TXNDC5下调可以抑制肾细胞癌细胞的增殖、迁移和侵袭,同时诱导细胞凋亡,这一过程与调节CCN2相关。
