Objective： The aim of our studywas to discuss changes in the clinicopathological characteristics of gastric cancer in Hehuang Valley of China in recent 10 years. Methods： A retrospective case-control study was perfo...Objective： The aim of our studywas to discuss changes in the clinicopathological characteristics of gastric cancer in Hehuang Valley of China in recent 10 years. Methods： A retrospective case-control study was performed on 2379 newly-diagnosed gastric cancer patients. All of them came from Hehuang Valley. The patients were divided into two groups [recent 5 years （R5Y） and late 5 years （L5Y）] from February 2003 to February 2013, and the clinicopathological data were surveyed retrospectively. Results： The constituent ratio of upper 1/3 gastric cancer in R5Y and L5Y was 33.5% and 20.7%, respectively, and it showed a significant difference between the two groups （X2 = 21.28, P = 0.00）, The constituent ratio of squamous carcinoma/adenosquamous carcinoma was 2.7% and 1.6%, respectively, and it also showed a significant differ- ence between two groups （X2 = 50.91, P = 0.00）. The constituent ratio of moderately-poorly differentiated/poorly differentiated gastric carcinoma was 84.2% and 50.2%, respectively, and it showed statistically significant difference （X2 = 30.28, P = 0.00）. The detection rate of early gastric cancer （EGC） was 1.47% （35/2379）. The constituent ratio of the types of Borrmann II and Borrmann IV of advanced gastric cancer （AGC） among 2379 cases was 47.6% and 40.8%, respectively, and that still showed statistically significant difference （X2 = 18,80, P = 0.00）. The constituent ratio of diffuse-type of gastric cancer in R5Y and L5Y was 36.2% and 30.8%, respectively, even there was a significant difference （X2 = 7.49, P = 0.01）. Furthermore, there were also significant differences in regional lymph nodes metastasis, perineural infiltration and vascular invasion （P 〈 0.05）. The positive detection rate of HER2, ER and PR was 14.88%, 17.23% and 15.93%, respectively. The constituent ratio of HP in two groups was 43.8% and 36.2% respectively, and it also showed a significant difference （~2 = 13.51, P = 0.00）. Conclusion： The pathogenic sites in gastric cancer change to the upper stomach in Hehuang Valley in recent 10 years, and the detection rate of squamous carcinoma/adenosquamous carcinoma reveals a sharp rise. Borrmann III is still one of the main types of advanced gastric cancer, but the detection rates of Borrmann II and IV are increasing. The main type of gastric cancer is the intestinal- type, but the ratio of diffuse-type is also increasing in recent 10 years. The HP detection rate is 40.65% （967/2379）, and it has a slight rise in recent 10 years. The detection rate of poorly differentiated adenocarcinoma is increasing despite the fact that the moderately/moderately-poorly differentiated adenocarcinoma is the main histologic types. High detection rates of lymph nodes metastasis, perineural infiltration and vascular invasion indicate poor prognosis in patients with gastric cancer. There is no change in HER2 positive rate, on the contrary, there are a little increase in ER and PR expression in Hehuang Valley.展开更多
Within the context of ground-air cooperation,the distributed formation trajectory tracking control problems for the Heterogeneous Multi-Agent Systems(HMASs)is studied.First,considering external disturbances and model ...Within the context of ground-air cooperation,the distributed formation trajectory tracking control problems for the Heterogeneous Multi-Agent Systems(HMASs)is studied.First,considering external disturbances and model uncertainties,a graph theory-based formation control protocol is designed for the HMASs consisting of Unmanned Aerial Vehicles(UAVs)and Unmanned Ground Vehicles(UGVs).Subsequently,a formation trajectory tracking control strategy employing adaptive Fractional-Order Sliding Mode Control(FOSMC)method is developed,and a Feedback Multilayer Fuzzy Neural Network(FMFNN)is introduced to estimate the lumped uncertainties.This approach empowers HMASs to adaptively follow the expected trajectory and adopt the designated formation configuration,even in the presence of various uncertainties.Additionally,an event-triggered mechanism is incorporated into the controller to reduce the update frequency of the controller and minimize the communication exchange among the agents,and the absence of Zeno behavior is rigorously demonstrated by an integral inequality analysis.Finally,to confirm the effectiveness of the proposed formation control protocol,some numerical simulations are presented.展开更多
Lung ischemia-reperfusion injury(IRI)stands as the primary culprit behind primary graft dysfunction(PGD)after lung transplantation,yet viable therapeutic options are lacking.In the present study,we used a murine hilar...Lung ischemia-reperfusion injury(IRI)stands as the primary culprit behind primary graft dysfunction(PGD)after lung transplantation,yet viable therapeutic options are lacking.In the present study,we used a murine hilar clamp(1 h)and reperfusion(3 h)model to study IRI.The left lung tissues were harvested for metabolomics,transcriptomics,and single-cell RNA sequencing.Metabolomics of plasma from human lung transplantation recipients was also performed.Lung histology,pulmonary function,pulmonary edema,and survival analysis were measured in mice.Integrative analysis of metabolomics and transcriptomics revealed a marked up-regulation of arachidonate 12-lipoxygenase(ALOX12)and its metabolite 12-hydroxyeicosatetraenoic acid(12-HETE),which played a pivotal role in promoting ferroptosis and neutrophil extracellular trap(NET)formation during lung IRI.Additionally,single-cell RNA sequencing revealed that ferroptosis predominantly occurred in pulmonary endothelial cells.Importantly,Alox12-knockout(KO)mice exhibited a notable decrease in ferroptosis,NET formation,and tissue injury.To investigate the interplay between endothelial ferroptosis and NET formation,a hypoxia/reoxygenation(HR)cell model using 2 human endothelial cell lines was established.By incubating conditioned medium from HR cell model with neutrophils,we found that the liberation of high mobility group box1(HMGB1)from endothelial cells undergoing ferroptosis facilitated the formation of NETs by activating the TLR4/MYD88 pathway.Last,the administration of ML355,a targeted inhibitor of Alox12,mitigated lung IRI in both murine hilar clamp/reperfusion and rat left lung transplant models.Collectively,our study indicates ALOX12 as a promising therapeutic strategy for lung IRI.展开更多
In the Research Article“Inhibition of ALOX12–12-HETE Alleviates Lung Ischemia–Reperfusion Injury by Reducing Endothelial Ferroptosis-Mediated Neutrophil Extracellular Trap Formation”,the authors have identified 2 ...In the Research Article“Inhibition of ALOX12–12-HETE Alleviates Lung Ischemia–Reperfusion Injury by Reducing Endothelial Ferroptosis-Mediated Neutrophil Extracellular Trap Formation”,the authors have identified 2 inadvertent errors in their figures[1].In the upper right panel of Fig.2A,the H&E image of Alox12-KO+Sham group was incorrect.Additionally,during the figure assembly process,the incorrect image of ACTB was used in Fig.6F and H.展开更多
Whether the alternated microbiota in the gut contribute to the risk of allograft rejection(AR)and pulmonary infection(Pl)in the setting of lung transplant recipients(LTRs)remains unexplored.A prospective multicenter c...Whether the alternated microbiota in the gut contribute to the risk of allograft rejection(AR)and pulmonary infection(Pl)in the setting of lung transplant recipients(LTRs)remains unexplored.A prospective multicenter cohort of LTRs was identified in the four lung transplant centers.Paired fecal and serum specimens were collected and divided into AR,Pl,and event-free(EF)groups according to the diagnosis at sampling.Fecal samples were determined by metagenomic sequencing.展开更多
基金Supported by a grant from the Basic Research Foundation of Qinghai Province(No.2011-Z-730)
文摘Objective： The aim of our studywas to discuss changes in the clinicopathological characteristics of gastric cancer in Hehuang Valley of China in recent 10 years. Methods： A retrospective case-control study was performed on 2379 newly-diagnosed gastric cancer patients. All of them came from Hehuang Valley. The patients were divided into two groups [recent 5 years （R5Y） and late 5 years （L5Y）] from February 2003 to February 2013, and the clinicopathological data were surveyed retrospectively. Results： The constituent ratio of upper 1/3 gastric cancer in R5Y and L5Y was 33.5% and 20.7%, respectively, and it showed a significant difference between the two groups （X2 = 21.28, P = 0.00）, The constituent ratio of squamous carcinoma/adenosquamous carcinoma was 2.7% and 1.6%, respectively, and it also showed a significant differ- ence between two groups （X2 = 50.91, P = 0.00）. The constituent ratio of moderately-poorly differentiated/poorly differentiated gastric carcinoma was 84.2% and 50.2%, respectively, and it showed statistically significant difference （X2 = 30.28, P = 0.00）. The detection rate of early gastric cancer （EGC） was 1.47% （35/2379）. The constituent ratio of the types of Borrmann II and Borrmann IV of advanced gastric cancer （AGC） among 2379 cases was 47.6% and 40.8%, respectively, and that still showed statistically significant difference （X2 = 18,80, P = 0.00）. The constituent ratio of diffuse-type of gastric cancer in R5Y and L5Y was 36.2% and 30.8%, respectively, even there was a significant difference （X2 = 7.49, P = 0.01）. Furthermore, there were also significant differences in regional lymph nodes metastasis, perineural infiltration and vascular invasion （P 〈 0.05）. The positive detection rate of HER2, ER and PR was 14.88%, 17.23% and 15.93%, respectively. The constituent ratio of HP in two groups was 43.8% and 36.2% respectively, and it also showed a significant difference （~2 = 13.51, P = 0.00）. Conclusion： The pathogenic sites in gastric cancer change to the upper stomach in Hehuang Valley in recent 10 years, and the detection rate of squamous carcinoma/adenosquamous carcinoma reveals a sharp rise. Borrmann III is still one of the main types of advanced gastric cancer, but the detection rates of Borrmann II and IV are increasing. The main type of gastric cancer is the intestinal- type, but the ratio of diffuse-type is also increasing in recent 10 years. The HP detection rate is 40.65% （967/2379）, and it has a slight rise in recent 10 years. The detection rate of poorly differentiated adenocarcinoma is increasing despite the fact that the moderately/moderately-poorly differentiated adenocarcinoma is the main histologic types. High detection rates of lymph nodes metastasis, perineural infiltration and vascular invasion indicate poor prognosis in patients with gastric cancer. There is no change in HER2 positive rate, on the contrary, there are a little increase in ER and PR expression in Hehuang Valley.
基金supported by the Beijing Municipal Science&Technology Commission China(No.Z19111000270000)the National Natural Science Foundation of China(Nos.62203050,51774042).
文摘Within the context of ground-air cooperation,the distributed formation trajectory tracking control problems for the Heterogeneous Multi-Agent Systems(HMASs)is studied.First,considering external disturbances and model uncertainties,a graph theory-based formation control protocol is designed for the HMASs consisting of Unmanned Aerial Vehicles(UAVs)and Unmanned Ground Vehicles(UGVs).Subsequently,a formation trajectory tracking control strategy employing adaptive Fractional-Order Sliding Mode Control(FOSMC)method is developed,and a Feedback Multilayer Fuzzy Neural Network(FMFNN)is introduced to estimate the lumped uncertainties.This approach empowers HMASs to adaptively follow the expected trajectory and adopt the designated formation configuration,even in the presence of various uncertainties.Additionally,an event-triggered mechanism is incorporated into the controller to reduce the update frequency of the controller and minimize the communication exchange among the agents,and the absence of Zeno behavior is rigorously demonstrated by an integral inequality analysis.Finally,to confirm the effectiveness of the proposed formation control protocol,some numerical simulations are presented.
基金supported by the National Key Research and Development Program of China(grant no.2024YFC3044600)Scientific and Technological Innovation Action Plans of Science and Technology Commission of Shanghai Municipality(nos.20DZ2253700,22Y2190050,and SHDC22021310-A)+1 种基金Ningbo Top Medical and Health Research Program(no.2022030208)The Young Talent Program of Shanghai Pulmonary Hospital(no.FKCY2302).
文摘Lung ischemia-reperfusion injury(IRI)stands as the primary culprit behind primary graft dysfunction(PGD)after lung transplantation,yet viable therapeutic options are lacking.In the present study,we used a murine hilar clamp(1 h)and reperfusion(3 h)model to study IRI.The left lung tissues were harvested for metabolomics,transcriptomics,and single-cell RNA sequencing.Metabolomics of plasma from human lung transplantation recipients was also performed.Lung histology,pulmonary function,pulmonary edema,and survival analysis were measured in mice.Integrative analysis of metabolomics and transcriptomics revealed a marked up-regulation of arachidonate 12-lipoxygenase(ALOX12)and its metabolite 12-hydroxyeicosatetraenoic acid(12-HETE),which played a pivotal role in promoting ferroptosis and neutrophil extracellular trap(NET)formation during lung IRI.Additionally,single-cell RNA sequencing revealed that ferroptosis predominantly occurred in pulmonary endothelial cells.Importantly,Alox12-knockout(KO)mice exhibited a notable decrease in ferroptosis,NET formation,and tissue injury.To investigate the interplay between endothelial ferroptosis and NET formation,a hypoxia/reoxygenation(HR)cell model using 2 human endothelial cell lines was established.By incubating conditioned medium from HR cell model with neutrophils,we found that the liberation of high mobility group box1(HMGB1)from endothelial cells undergoing ferroptosis facilitated the formation of NETs by activating the TLR4/MYD88 pathway.Last,the administration of ML355,a targeted inhibitor of Alox12,mitigated lung IRI in both murine hilar clamp/reperfusion and rat left lung transplant models.Collectively,our study indicates ALOX12 as a promising therapeutic strategy for lung IRI.
文摘In the Research Article“Inhibition of ALOX12–12-HETE Alleviates Lung Ischemia–Reperfusion Injury by Reducing Endothelial Ferroptosis-Mediated Neutrophil Extracellular Trap Formation”,the authors have identified 2 inadvertent errors in their figures[1].In the upper right panel of Fig.2A,the H&E image of Alox12-KO+Sham group was incorrect.Additionally,during the figure assembly process,the incorrect image of ACTB was used in Fig.6F and H.
基金This work was supported by the National Key Research and Development Program of China(2022YFC2504800)National Natural Science Foundation of China(Nos.82072257 and 82202543)+2 种基金Scientific and Technological Innovation action Plan of Science and Technology Commission of Shanghai Municipality(Nos.20DZ2253700,20DZ2272000,21410750500,and 22Y21900500)Shanghai Municipal Health Commission(shslczdzk01001)Guangxi Department of Science and Technology Foundation(No.2020GXNSFGA238001).
文摘Whether the alternated microbiota in the gut contribute to the risk of allograft rejection(AR)and pulmonary infection(Pl)in the setting of lung transplant recipients(LTRs)remains unexplored.A prospective multicenter cohort of LTRs was identified in the four lung transplant centers.Paired fecal and serum specimens were collected and divided into AR,Pl,and event-free(EF)groups according to the diagnosis at sampling.Fecal samples were determined by metagenomic sequencing.
