Hyla tsinlingensis(Hu et al.,1966)is endemic to China,and is widely distributed in the Qinling and Dabie Mountains.Previous studies have suggested that genetic differentiation and geographical isolation exist between ...Hyla tsinlingensis(Hu et al.,1966)is endemic to China,and is widely distributed in the Qinling and Dabie Mountains.Previous studies have suggested that genetic differentiation and geographical isolation exist between the Qinling Mountains population and Dabie Mountains population of H.tsinlingensis,and that the Dabie Mountains population is a cryptic species.Several Hyla specimens were collected from the Dabie Mountains of western Anhui Province,China during herpetological surveys from 2022‒2024;these specimens belonged to a species previously identified as H.tsinlingensis.Herein,we utilized phylogenetic analyses,morphological comparisons,and advertisement call comparisons,revealing significant differences from those of other known congeners;thus,we describe them as a new species.The discovery of this species implies that there are currently 18 identified species in the genus Hyla,7 of which are found in China.展开更多
Successful non-native species can exhibit rapid divergence of key phenotypic traits to facilitate population persistence and further range expansion.Such phenotypic shifts can be sex-dependent as males and females usu...Successful non-native species can exhibit rapid divergence of key phenotypic traits to facilitate population persistence and further range expansion.Such phenotypic shifts can be sex-dependent as males and females usually have distinct reproductive and dispersal modes responding to relevant selective forces.Here,we showed significant phenotypic divergence in a set of fitness-related traits among males but not among females in the introduced oriental fire-bellied toads(Bombina orientalis),which established a non-native population in Beijing after being introduced in 1927.Specifically,compared with the native counterparts,introduced males displayed younger age and reduced body size,but increased hindlimb length and eye size after controlling the age effect.As the toads have male-biased sex ratio and male-biased dispersal during breeding season,the observed shifts may suggest that the male-biased selective forces have induced increased energy allocation to dispersal and foraging,and thus decreased allocation to maintain longevity in males.Overall,our study provides evidence for the modification of fitness-related phenotypic traits in a non-native anuran population compared with native populations and extends our understanding of key trait divergence in established non-native amphibian under sexual-unbalanced selective forces.展开更多
Precipitation at grain boundaries is typically not regarded as an efficient method for strengthening materials since it can induce grain boundary embrittlement, which detrimentally affects ductility. In this research,...Precipitation at grain boundaries is typically not regarded as an efficient method for strengthening materials since it can induce grain boundary embrittlement, which detrimentally affects ductility. In this research, we developed a multi-principal element alloy (MPEA) with the composition Cr_(30)Co_(30)Ni_(30)Al_(5)Ti_(5) (at.%), incorporating both intragranular and intergranular nanoprecipitates. Utilizing multiscale, three-dimensional, and in-situ electron microscopy techniques, coupled with computational simulations, we established that intergranular nanoprecipitation in this material plays a crucial role in enhancing strength and promoting dislocation plasticity. The structure of intergranular nanoprecipitation comprises multiple phases with varying composition and structure. Despite the diversity, the crystal planes conducive to the easy glide of dislocations are well-matched, allowing for the sustained continuity of dislocation slipping across different phase structures. Simultaneously, this structure generates an undulated stress field near grain boundaries, amplifying the strengthening effect and facilitating multiple slip and cross-slip during deformation. Consequently, it promotes the proliferation and storage of dislocations. As a result, our material exhibits a yield strength of approximately 1010 MPa and an ultimate tensile strength of around 1500 MPa, accompanied by a significant fracture elongation of 41 %. Our findings illuminate the potential for harnessing intergranular nanoprecipitation to optimize the strength-ductility trade-off in MPEAs, emphasizing the strategy of leveraging complex compositions for the design of sophisticated functional microstructures.展开更多
Tropical cyclones(TCs)are complex and powerful weather systems,and accurately forecasting their path,structure,and intensity remains a critical focus and challenge in meteorological research.In this paper,we propose a...Tropical cyclones(TCs)are complex and powerful weather systems,and accurately forecasting their path,structure,and intensity remains a critical focus and challenge in meteorological research.In this paper,we propose an Attention Spatio-Temporal predictive Generative Adversarial Network(AST-GAN)model for predicting the temporal and spatial distribution of TCs.The model forecasts the spatial distribution of TC wind speeds for the next 15 hours at 3-hour intervals,emphasizing the cyclone's center,high wind-speed areas,and its asymmetric structure.To effectively capture spatiotemporal feature transfer at different time steps,we employ a channel attention mechanism for feature selection,enhancing model performance and reducing parameter redundancy.We utilized High-Resolution Weather Research and Forecasting(HWRF)data to train our model,allowing it to assimilate a wide range of TC motion patterns.The model is versatile and can be applied to various complex scenarios,such as multiple TCs moving simultaneously or TCs approaching landfall.Our proposed model demonstrates superior forecasting performance,achieving a root-mean-square error(RMSE)of 0.71 m s^(-1)for overall wind speed and 2.74 m s^(-1)for maximum wind speed when benchmarked against ground truth data from HWRF.Furthermore,the model underwent optimization and independent testing using ERA5reanalysis data,showcasing its stability and scalability.After fine-tuning on the ERA5 dataset,the model achieved an RMSE of 1.33 m s^(-1)for wind speed and 1.75 m s^(-1)for maximum wind speed.The AST-GAN model outperforms other state-of-the-art models in RMSE on both the HWRF and ERA5 datasets,maintaining its superior performance and demonstrating its effectiveness for spatiotemporal prediction of TCs.展开更多
Bacterial infections pose a significant threat to human health and entail substantial economic losses.Due to the broad-spectrum antibacterial effect and low susceptibility to drug resistance,photodynamic therapy(PDT),...Bacterial infections pose a significant threat to human health and entail substantial economic losses.Due to the broad-spectrum antibacterial effect and low susceptibility to drug resistance,photodynamic therapy(PDT),a nontraditional antibacterial approach,has garnered a lot of attention.In PDT,the selection of photosensitizer(PS)is crucial because it directly affects the efficiency and safety of the treatment.As a versatile fluorophore,the advantages of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene(BODIPY)used as a PS for antibacterial PDT are mainly reflected in its high quantum yield of singlet oxygen,easy modification,and exceptional photostability.Through strategic chemical modifications of the BODIPY structures,it is possible to enhance their photodynamic antibacterial activity and refine their selectivity for bacterial killing.This review focuses on the application of BODIPY-based PSs for treating bacterial infections.According to the design strategies of photodynamic antibacterial materials incorporating BODIPY,a variety of representative therapeutic agents having emerged in recent years are classified and discussed,aiming to offer insights for future research and development in this field.展开更多
Nitrogen gas pressure sintering was successfully employed to achieve the in-situ formation of Si_(3)N_(4)-bonded MgO-C refractories.The primary objective was to investigate the influence of different gas pressures on ...Nitrogen gas pressure sintering was successfully employed to achieve the in-situ formation of Si_(3)N_(4)-bonded MgO-C refractories.The primary objective was to investigate the influence of different gas pressures on the mechanical properties and microstructure of MgO-C refractories.The results indicate that higher nitrogen pressure promotes the transformation of silicon nitride from theαphase to theβphase.This phase transition positively impacts the mechanical properties of Si_(3)N_(4)-bonded MgO-C refractories,leading to an enhancement in their overall strength.Notably,when the nitrogen pressure was set at 3 MPa,exceptional compressive strength of 109.7 MPa and an elastic modulus of 142.4 GPa were achieved by these prepared refractories.These findings highlight the great potential for utilizing gas pressure sintered Si_(3)N_(4)-MgO-C refractories.展开更多
Large bile duct stone(> 10 mm) or multiple stones(≥ 3) are challenging for endoscopists. Endoscopic sphincterotomy(EST) is a routine therapeutic endoscopic retrograde cholangiopancreatography(ERCP) procedure usual...Large bile duct stone(> 10 mm) or multiple stones(≥ 3) are challenging for endoscopists. Endoscopic sphincterotomy(EST) is a routine therapeutic endoscopic retrograde cholangiopancreatography(ERCP) procedure usually used. It is safe and effective, but severe perforation or massive bleeding are the main causes of mortality. Because of the permanent destroy ofOddi sphincter, the use of EST is still controversial. Endoscopic papillary balloon dilation(EPBD) gives another way to open the sphincter. Less incidence of bleeding, perforation and partly preserving the Oddi sphincter's function are the main advantages. But high incidence of post-ERCP pancreatitis becomes a predominant problem. According to the anatomical feature of Oddi sphincter, limited EST + EPBD seems a more reasonable procedure. Compared to the former two procedures, it makes the stone extraction process much easier with lower incidences of short-term and long-term complications.展开更多
Aloin is a small-molecule drug well known for its protective actions in various models of damage.Traumatic brain injury(TBI)-induced cerebral edema from secondary damage caused by disruption of the blood-brain barrier...Aloin is a small-molecule drug well known for its protective actions in various models of damage.Traumatic brain injury(TBI)-induced cerebral edema from secondary damage caused by disruption of the blood-brain barrier(BBB) often leads to an adverse prognosis.Since the role of aloin in maintaining the integrity of the BBB after TBI remains unclear,we explored the protective effects of aloin on the BBB using in vivo and in vitro TBI models.Adult male C57 BL/6 mice underwent controlled cortical impact injury,and mouse brain capillary endothelial bEnd.3 cells underwent biaxial stretch injury,then both received aloin treatment.In the animal experiments,we found 20 mg/kg aloin to be the optimum concentration to decrease cerebral edema,decrease disruption of the BBB,and improve neurobehavioral performance after cortical impact injury.In the cellular studies,the optimum concentration of 40 μg/mL aloin reduced apoptosis and reversed the loss of tight junctions by reducing the reactive oxygen species levels and changes in mitochondrial membrane potential after stretch injury.The mechanisms may be that aloin downregulates the phosphorylation of p38 mitogen-activated protein kinase,the activation of p65 nuclear factor-kappa B,and the ratios of B cell lymphoma(Bcl)-2-associated X protein/Bcl-2 and cleaved caspase-3/caspase-3.We conclude that aloin exhibits these protective effects on the BBB after TBI through its anti-oxidative stress and anti-apoptotic properties in mouse brain capillary endothelial cells.Aloin may thus be a promising therapeutic drug for TBI.展开更多
Multi-principal element solid solutions are prone to develop local chemical inhomogeneities,i.e.,chemi-cal order/clustering and/or compositional undulation.However,these structural details from short-range(first coupl...Multi-principal element solid solutions are prone to develop local chemical inhomogeneities,i.e.,chemi-cal order/clustering and/or compositional undulation.However,these structural details from short-range(first couple of nearest-neighbor atomic shells)to nanometer length scale are very challenging to re-solve in both experimental characterization and computer simulations.For instance,Monte Carlo model-ing based on density-functional-theory calculations is severely limited by the sample size and the sim-ulation steps practical in the simulations.Adopting the cluster expansion approach,here we systemati-cally reveal the local chemical inhomogeneity,including chemical order and compositional fluctuation,in three representative equiatomic TiZrNb-based body-centered cubic refractory high-entropy alloys(HEAs):TiZrNb,TiZrHfNb and TiZrHfNbTa.Ti-Zr pairs are found to exhibit the highest degree of chemical pref-erence among all atomic pairs.Such chemical short-range order(CSRO)induces an accompanying com-positional undulation,both extending to characteristic dimensions of the order of one nanometer.The chemical inhomogeneity trend uncovered for this series of TiZrNb-based HEAs is expected to impact their mechanical properties;e.g.,incorporating the CSRO effects in a current model significantly improves its agreement with experimental measured yield strength.展开更多
The demand for low-carbon MgO–C refractories is ever growing to meet the development of advanced steelmaking technologies and efficient energy conservation.Meanwhile,to improve the oxidation resistance and inhibit th...The demand for low-carbon MgO–C refractories is ever growing to meet the development of advanced steelmaking technologies and efficient energy conservation.Meanwhile,to improve the oxidation resistance and inhibit the weakness of low-carbon MgO–C refractories,antioxidants are necessary.The application of ternary carbides that focused on improving the oxidation performance of MgO–C refractories has been explored,and the ternary carbides including Al_(4)O_(4)C,Al8B_(4)C_(7),Al_(4)SiC_(4),Ti_(2)AlC,Ti_(3)AlC_(2),and Ti_(3)SiC_(2)have been proved effective.The crystal structure,physical properties,oxidation behavior,and synthesis of these ternary carbides were summarized,and their oxidation mechanism in assisting antioxidation of MgO–C refractories was discussed.In addition,the potential aspects related to the usage and development of ternary carbides in low-carbon MgO–C refractories were proposed.展开更多
Direct infusion mass spectrometry(DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots(DBS).However,DIMS sometimes generated false-positive r...Direct infusion mass spectrometry(DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots(DBS).However,DIMS sometimes generated false-positive results for analysis of amino acids.In this work,we utilized a stable isotope derivatization method,combining with liquid chromatography tandem mass spectrometry(SID-LC-MS),to improve the specificity for screening amino acids in DBS specimens.A pair of isotope reagents,p-(dimethylamino)phenyl isothiocyanate(DMAP-NCS) and 4-isothiocyanato-N,N-bis(methyl-[2H2])aniline([2H4]DMAP-NCS),was synthesized and used to label amino acids in DBS specimens.The [2H4]DMAP-NCS labelled amino acid standards were used as internal standards to compensate the matrix effect.This method was validated by measuring linearity,recovery and accuracy.The results showed that the developed SID-LC-MS method can be used for sensitive and selective determination of 12 diagnostically important amino acids in DBS specimens.展开更多
Our previous study showed that cell cycle exit and neuronal differentiation 1(CEND1)may participate in neural stem cell cycle exit and oriented differentiation.However,whether CEND1-transfected neural stem cells can i...Our previous study showed that cell cycle exit and neuronal differentiation 1(CEND1)may participate in neural stem cell cycle exit and oriented differentiation.However,whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear.In this study,we performed quantitative proteomic analysis and found that after traumatic brain injury,CEND1 expression was downregulated in mouse brain tissue.Three days after traumatic brain injury,we transplanted CEND1-transfected neural stem cells into the area surrounding the injury site.We found that at 5 weeks after traumatic brain injury,transplantation of CEND1-transfected neural stem cells markedly alleviated brain atrophy and greatly improved neurological function.In vivo and in vitro results indicate that CEND1 overexpression inhibited the proliferation of neural stem cells,but significantly promoted their neuronal differentiation.Additionally,CEND1 overexpression reduced protein levels of Notch1 and cyclin D1,but increased levels of p21 in CEND1-transfected neural stem cells.Treatment with CEND1-transfected neural stem cells was superior to similar treatment without CEND1 transfection.These findings suggest that transplantation of CEND1-transfected neural stem cells is a promising cell therapy for traumatic brain injury.This study was approved by the Animal Ethics Committee of the School of Biomedical Engineering of Shanghai Jiao Tong University,China(approval No.2016034)on November 25,2016.展开更多
Electron beam melting(EBM)is a promising technology to manufacture various alloys with outstanding properties;however,the number of available alloys is limited.We propose in situ alloying to accelerate the development...Electron beam melting(EBM)is a promising technology to manufacture various alloys with outstanding properties;however,the number of available alloys is limited.We propose in situ alloying to accelerate the development of advanced and novel alloys,based on thermophysical calculations and CALPHAD approach,during the EBM process.We demonstrate our concept through the design and fabrication of high entropy alloys(HEAs).Three CoCrFeNiMn-xTi(x=0.18,0.50,2.00,in molar%)HEAs are manufactured.EBM-built HEAs achieve a homogeneous distribution of elements while forming multiphase alloys resulted from the hot powder bed.The topological structures formed by secondary phases contribute to an increase in the hardness of EBM-built HEAs up to 900 HV1.Considering alloy design,a systematic analysis on Co Cr Fe Ni Mn-0.18 Ti HEA elucidates the microstructural evolution in detail.These findings provide a deep understanding of in situ alloying and pave the way to develop new alloys specific to the EBM process.展开更多
Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneou...Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.展开更多
Studies have found that the phosphatase actin regulatory factor 1 expression can be related to stroke,but it remains unclear whether changes in phosphatase actin regulatory factor 1 expression also play a role in trau...Studies have found that the phosphatase actin regulatory factor 1 expression can be related to stroke,but it remains unclear whether changes in phosphatase actin regulatory factor 1 expression also play a role in traumatic brain injury.In this study we found that,in a mouse model of traumatic brain injury induced by controlled cortical impact,phosphatase actin regulatory factor 1 expression is increased in endothelial cells,neurons,astrocytes,and microglia.When we overexpressed phosphatase actin regulatory factor 1 by injection an adeno-associated virus vector into the contused area in the traumatic brain injury mice,the water content of the brain tissue increased.However,when phosphatase actin regulatory factor 1 was knocked down,the water content decreased.We also found that inhibiting phosphatase actin regulatory factor 1 expression regulated the nuclear factor kappa B signaling pathway,decreased blood-brain barrier permeability,reduced aquaporin 4 and intercellular adhesion molecule 1 expression,inhibited neuroinflammation,and neuronal apoptosis,thereby improving neurological function.The findings from this study indicate that phosphatase actin regulatory factor 1 may be a potential therapeutic target for traumatic brain injury.展开更多
The increasing energy requirements to power the modern world has driven active research into more advanced electrochemical energy storage devices(EESD)with both high energy densities and power densities.Wide range of ...The increasing energy requirements to power the modern world has driven active research into more advanced electrochemical energy storage devices(EESD)with both high energy densities and power densities.Wide range of newly discovered materials with promising electrochemical properties has shown great potential for next-generation devices,but their performance is normally associated with contradicting demands of thin electrodes and high mass loading that can be hardly achieved for practical applications.Design of three-dimensional(3D)porous electrodes can increase the mass loading while maintaining the effective charge transport even with thick electrodes,which has proven to be efficient to overcome the limitations.3D structures have also been demonstrated excellent structural stability to withstand strong strains and stresses generated during charge/discharge cycle.3D printing,which can fabricate various delicate and complex structural designs,thus offering brand-new opportunities for the rational design and facile construction of next-generation EESDs.The recent developments in 3D printing of next-generation EESDs with high performance are reviewed.Advanced/multiscale electrode structures,such as hierarchically porous structure that can be constructed via high-resolution 3D printing or with post-treatment,are further emphasized.The ability of current 3D printing techniques to fulfill multimaterial printing to fulfill simple packaging will be covered.展开更多
基金The Comprehensive Monitoring Station for National Ecological Quality in the Dabie Mountains(Forest)Ecological Monitoring for the Years 2024‒2025(ZF2024-18-1376)。
文摘Hyla tsinlingensis(Hu et al.,1966)is endemic to China,and is widely distributed in the Qinling and Dabie Mountains.Previous studies have suggested that genetic differentiation and geographical isolation exist between the Qinling Mountains population and Dabie Mountains population of H.tsinlingensis,and that the Dabie Mountains population is a cryptic species.Several Hyla specimens were collected from the Dabie Mountains of western Anhui Province,China during herpetological surveys from 2022‒2024;these specimens belonged to a species previously identified as H.tsinlingensis.Herein,we utilized phylogenetic analyses,morphological comparisons,and advertisement call comparisons,revealing significant differences from those of other known congeners;thus,we describe them as a new species.The discovery of this species implies that there are currently 18 identified species in the genus Hyla,7 of which are found in China.
基金supported by the National Natural Science Foundation of China (32171657,32301459)the grant from Institute of Zoology,Chinese Academy of Sciences (2023IOZ0104)the grant from Youth Innovation Promotion Association of Chinese Academy of Sciences (Y201920)。
文摘Successful non-native species can exhibit rapid divergence of key phenotypic traits to facilitate population persistence and further range expansion.Such phenotypic shifts can be sex-dependent as males and females usually have distinct reproductive and dispersal modes responding to relevant selective forces.Here,we showed significant phenotypic divergence in a set of fitness-related traits among males but not among females in the introduced oriental fire-bellied toads(Bombina orientalis),which established a non-native population in Beijing after being introduced in 1927.Specifically,compared with the native counterparts,introduced males displayed younger age and reduced body size,but increased hindlimb length and eye size after controlling the age effect.As the toads have male-biased sex ratio and male-biased dispersal during breeding season,the observed shifts may suggest that the male-biased selective forces have induced increased energy allocation to dispersal and foraging,and thus decreased allocation to maintain longevity in males.Overall,our study provides evidence for the modification of fitness-related phenotypic traits in a non-native anuran population compared with native populations and extends our understanding of key trait divergence in established non-native amphibian under sexual-unbalanced selective forces.
基金supported by the National Science Fund for Distinguished Young Scholars of China(No.52325102)the Natural Science Foundation of Zhejiang Province,China(No.LZ22E010001)the National Key R&D Program of China(No.2023YFB2405802).
文摘Precipitation at grain boundaries is typically not regarded as an efficient method for strengthening materials since it can induce grain boundary embrittlement, which detrimentally affects ductility. In this research, we developed a multi-principal element alloy (MPEA) with the composition Cr_(30)Co_(30)Ni_(30)Al_(5)Ti_(5) (at.%), incorporating both intragranular and intergranular nanoprecipitates. Utilizing multiscale, three-dimensional, and in-situ electron microscopy techniques, coupled with computational simulations, we established that intergranular nanoprecipitation in this material plays a crucial role in enhancing strength and promoting dislocation plasticity. The structure of intergranular nanoprecipitation comprises multiple phases with varying composition and structure. Despite the diversity, the crystal planes conducive to the easy glide of dislocations are well-matched, allowing for the sustained continuity of dislocation slipping across different phase structures. Simultaneously, this structure generates an undulated stress field near grain boundaries, amplifying the strengthening effect and facilitating multiple slip and cross-slip during deformation. Consequently, it promotes the proliferation and storage of dislocations. As a result, our material exhibits a yield strength of approximately 1010 MPa and an ultimate tensile strength of around 1500 MPa, accompanied by a significant fracture elongation of 41 %. Our findings illuminate the potential for harnessing intergranular nanoprecipitation to optimize the strength-ductility trade-off in MPEAs, emphasizing the strategy of leveraging complex compositions for the design of sophisticated functional microstructures.
基金supported by the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(NO.SML2021SP201)the National Natural Science Foundation of China(Grant No.42306200 and 42306216)+2 种基金the National Key Research and Development Program of China(Grant No.2023YFC3008100)the Innovation Group Project of the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(Grant No.311021004)the Oceanic Interdisciplinary Program of Shanghai Jiao Tong University(Project No.SL2021ZD203)。
文摘Tropical cyclones(TCs)are complex and powerful weather systems,and accurately forecasting their path,structure,and intensity remains a critical focus and challenge in meteorological research.In this paper,we propose an Attention Spatio-Temporal predictive Generative Adversarial Network(AST-GAN)model for predicting the temporal and spatial distribution of TCs.The model forecasts the spatial distribution of TC wind speeds for the next 15 hours at 3-hour intervals,emphasizing the cyclone's center,high wind-speed areas,and its asymmetric structure.To effectively capture spatiotemporal feature transfer at different time steps,we employ a channel attention mechanism for feature selection,enhancing model performance and reducing parameter redundancy.We utilized High-Resolution Weather Research and Forecasting(HWRF)data to train our model,allowing it to assimilate a wide range of TC motion patterns.The model is versatile and can be applied to various complex scenarios,such as multiple TCs moving simultaneously or TCs approaching landfall.Our proposed model demonstrates superior forecasting performance,achieving a root-mean-square error(RMSE)of 0.71 m s^(-1)for overall wind speed and 2.74 m s^(-1)for maximum wind speed when benchmarked against ground truth data from HWRF.Furthermore,the model underwent optimization and independent testing using ERA5reanalysis data,showcasing its stability and scalability.After fine-tuning on the ERA5 dataset,the model achieved an RMSE of 1.33 m s^(-1)for wind speed and 1.75 m s^(-1)for maximum wind speed.The AST-GAN model outperforms other state-of-the-art models in RMSE on both the HWRF and ERA5 datasets,maintaining its superior performance and demonstrating its effectiveness for spatiotemporal prediction of TCs.
文摘Bacterial infections pose a significant threat to human health and entail substantial economic losses.Due to the broad-spectrum antibacterial effect and low susceptibility to drug resistance,photodynamic therapy(PDT),a nontraditional antibacterial approach,has garnered a lot of attention.In PDT,the selection of photosensitizer(PS)is crucial because it directly affects the efficiency and safety of the treatment.As a versatile fluorophore,the advantages of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene(BODIPY)used as a PS for antibacterial PDT are mainly reflected in its high quantum yield of singlet oxygen,easy modification,and exceptional photostability.Through strategic chemical modifications of the BODIPY structures,it is possible to enhance their photodynamic antibacterial activity and refine their selectivity for bacterial killing.This review focuses on the application of BODIPY-based PSs for treating bacterial infections.According to the design strategies of photodynamic antibacterial materials incorporating BODIPY,a variety of representative therapeutic agents having emerged in recent years are classified and discussed,aiming to offer insights for future research and development in this field.
基金the financial support from the National Natural Science Foundation of China(U21A2057 and 52402034)the Key Research and Development Program of Hubei Province(2023BAB106).
文摘Nitrogen gas pressure sintering was successfully employed to achieve the in-situ formation of Si_(3)N_(4)-bonded MgO-C refractories.The primary objective was to investigate the influence of different gas pressures on the mechanical properties and microstructure of MgO-C refractories.The results indicate that higher nitrogen pressure promotes the transformation of silicon nitride from theαphase to theβphase.This phase transition positively impacts the mechanical properties of Si_(3)N_(4)-bonded MgO-C refractories,leading to an enhancement in their overall strength.Notably,when the nitrogen pressure was set at 3 MPa,exceptional compressive strength of 109.7 MPa and an elastic modulus of 142.4 GPa were achieved by these prepared refractories.These findings highlight the great potential for utilizing gas pressure sintered Si_(3)N_(4)-MgO-C refractories.
基金supported by National Natural Science Foundation of China,China(No.52101138)Natural Science Foundation of Hubei Province,China(No.2020CFB259)+7 种基金Shenzhen Science and Technology Program,China(No.JCYJ20220530160813032)State Key Lab of Advanced Metals and Materials,China(No.2020-Z01)State Key Laboratory for Mechanical Behavior of Materials,China(No.20202205)Guangdong Basic and Applied Basic Research Foundation,China(Nos.2020A1515110531,2021A1515111122)State Key Laboratory of Materials Processing and Die&Mold Technology,Huazhong University of Science and Technology,China(No.P2021-021)support from the National Natural Science Foundation of China(No.12004294)National Youth Talents Programsupport by Center for Alloy Innovation and Design(CAID)and HPC platform of Xi’an Jiaotong University。
文摘Large bile duct stone(> 10 mm) or multiple stones(≥ 3) are challenging for endoscopists. Endoscopic sphincterotomy(EST) is a routine therapeutic endoscopic retrograde cholangiopancreatography(ERCP) procedure usually used. It is safe and effective, but severe perforation or massive bleeding are the main causes of mortality. Because of the permanent destroy ofOddi sphincter, the use of EST is still controversial. Endoscopic papillary balloon dilation(EPBD) gives another way to open the sphincter. Less incidence of bleeding, perforation and partly preserving the Oddi sphincter's function are the main advantages. But high incidence of post-ERCP pancreatitis becomes a predominant problem. According to the anatomical feature of Oddi sphincter, limited EST + EPBD seems a more reasonable procedure. Compared to the former two procedures, it makes the stone extraction process much easier with lower incidences of short-term and long-term complications.
基金supported by the National Natural Science Foundation of China (81671207,81701895,and 81501048)the Shanghai Jiao Tong University Medicine-Engineering Research Fund (YG2016QN20).
文摘Aloin is a small-molecule drug well known for its protective actions in various models of damage.Traumatic brain injury(TBI)-induced cerebral edema from secondary damage caused by disruption of the blood-brain barrier(BBB) often leads to an adverse prognosis.Since the role of aloin in maintaining the integrity of the BBB after TBI remains unclear,we explored the protective effects of aloin on the BBB using in vivo and in vitro TBI models.Adult male C57 BL/6 mice underwent controlled cortical impact injury,and mouse brain capillary endothelial bEnd.3 cells underwent biaxial stretch injury,then both received aloin treatment.In the animal experiments,we found 20 mg/kg aloin to be the optimum concentration to decrease cerebral edema,decrease disruption of the BBB,and improve neurobehavioral performance after cortical impact injury.In the cellular studies,the optimum concentration of 40 μg/mL aloin reduced apoptosis and reversed the loss of tight junctions by reducing the reactive oxygen species levels and changes in mitochondrial membrane potential after stretch injury.The mechanisms may be that aloin downregulates the phosphorylation of p38 mitogen-activated protein kinase,the activation of p65 nuclear factor-kappa B,and the ratios of B cell lymphoma(Bcl)-2-associated X protein/Bcl-2 and cleaved caspase-3/caspase-3.We conclude that aloin exhibits these protective effects on the BBB after TBI through its anti-oxidative stress and anti-apoptotic properties in mouse brain capillary endothelial cells.Aloin may thus be a promising therapeutic drug for TBI.
基金J.D.and E.M.acknowledge XJTU for hosting their research at the Center for Alloy Innovation and Design(CAID).This work was funded by the Natural Science Foundation of China(No.12004294)National Youth Talents Program and the HPC platform of Xi’an Jiaotong University。
文摘Multi-principal element solid solutions are prone to develop local chemical inhomogeneities,i.e.,chemi-cal order/clustering and/or compositional undulation.However,these structural details from short-range(first couple of nearest-neighbor atomic shells)to nanometer length scale are very challenging to re-solve in both experimental characterization and computer simulations.For instance,Monte Carlo model-ing based on density-functional-theory calculations is severely limited by the sample size and the sim-ulation steps practical in the simulations.Adopting the cluster expansion approach,here we systemati-cally reveal the local chemical inhomogeneity,including chemical order and compositional fluctuation,in three representative equiatomic TiZrNb-based body-centered cubic refractory high-entropy alloys(HEAs):TiZrNb,TiZrHfNb and TiZrHfNbTa.Ti-Zr pairs are found to exhibit the highest degree of chemical pref-erence among all atomic pairs.Such chemical short-range order(CSRO)induces an accompanying com-positional undulation,both extending to characteristic dimensions of the order of one nanometer.The chemical inhomogeneity trend uncovered for this series of TiZrNb-based HEAs is expected to impact their mechanical properties;e.g.,incorporating the CSRO effects in a current model significantly improves its agreement with experimental measured yield strength.
基金The authors acknowledge the financial support from the National Natural Science Foundation of China(U20A20239)Natural Science Foundation of Hubei Province(2020CFB692).
文摘The demand for low-carbon MgO–C refractories is ever growing to meet the development of advanced steelmaking technologies and efficient energy conservation.Meanwhile,to improve the oxidation resistance and inhibit the weakness of low-carbon MgO–C refractories,antioxidants are necessary.The application of ternary carbides that focused on improving the oxidation performance of MgO–C refractories has been explored,and the ternary carbides including Al_(4)O_(4)C,Al8B_(4)C_(7),Al_(4)SiC_(4),Ti_(2)AlC,Ti_(3)AlC_(2),and Ti_(3)SiC_(2)have been proved effective.The crystal structure,physical properties,oxidation behavior,and synthesis of these ternary carbides were summarized,and their oxidation mechanism in assisting antioxidation of MgO–C refractories was discussed.In addition,the potential aspects related to the usage and development of ternary carbides in low-carbon MgO–C refractories were proposed.
基金supported by the National Key R&D Program of China(No.2018YFA0900400)the National Natural ScienceFoundation of China(Nos.21635006,31670373,21721005,21904099)the Postdoctoral Science Foundation of China(No.2018M642893)。
文摘Direct infusion mass spectrometry(DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots(DBS).However,DIMS sometimes generated false-positive results for analysis of amino acids.In this work,we utilized a stable isotope derivatization method,combining with liquid chromatography tandem mass spectrometry(SID-LC-MS),to improve the specificity for screening amino acids in DBS specimens.A pair of isotope reagents,p-(dimethylamino)phenyl isothiocyanate(DMAP-NCS) and 4-isothiocyanato-N,N-bis(methyl-[2H2])aniline([2H4]DMAP-NCS),was synthesized and used to label amino acids in DBS specimens.The [2H4]DMAP-NCS labelled amino acid standards were used as internal standards to compensate the matrix effect.This method was validated by measuring linearity,recovery and accuracy.The results showed that the developed SID-LC-MS method can be used for sensitive and selective determination of 12 diagnostically important amino acids in DBS specimens.
基金supported by the National Natural Science Foundation of China,No.81701895Shanghai Jiao Tong University Medicine-Engineering Research Fund,China,No.YG2016QN20(both to FY)。
文摘Our previous study showed that cell cycle exit and neuronal differentiation 1(CEND1)may participate in neural stem cell cycle exit and oriented differentiation.However,whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear.In this study,we performed quantitative proteomic analysis and found that after traumatic brain injury,CEND1 expression was downregulated in mouse brain tissue.Three days after traumatic brain injury,we transplanted CEND1-transfected neural stem cells into the area surrounding the injury site.We found that at 5 weeks after traumatic brain injury,transplantation of CEND1-transfected neural stem cells markedly alleviated brain atrophy and greatly improved neurological function.In vivo and in vitro results indicate that CEND1 overexpression inhibited the proliferation of neural stem cells,but significantly promoted their neuronal differentiation.Additionally,CEND1 overexpression reduced protein levels of Notch1 and cyclin D1,but increased levels of p21 in CEND1-transfected neural stem cells.Treatment with CEND1-transfected neural stem cells was superior to similar treatment without CEND1 transfection.These findings suggest that transplantation of CEND1-transfected neural stem cells is a promising cell therapy for traumatic brain injury.This study was approved by the Animal Ethics Committee of the School of Biomedical Engineering of Shanghai Jiao Tong University,China(approval No.2016034)on November 25,2016.
基金financially supported by the A^(*)STAR Additive Manufacturing Centre(AMC)Initiative:Work Package 1(High Temperature Materials Development for 3D Additive Manufacturing,Grant No.1426800088)。
文摘Electron beam melting(EBM)is a promising technology to manufacture various alloys with outstanding properties;however,the number of available alloys is limited.We propose in situ alloying to accelerate the development of advanced and novel alloys,based on thermophysical calculations and CALPHAD approach,during the EBM process.We demonstrate our concept through the design and fabrication of high entropy alloys(HEAs).Three CoCrFeNiMn-xTi(x=0.18,0.50,2.00,in molar%)HEAs are manufactured.EBM-built HEAs achieve a homogeneous distribution of elements while forming multiphase alloys resulted from the hot powder bed.The topological structures formed by secondary phases contribute to an increase in the hardness of EBM-built HEAs up to 900 HV1.Considering alloy design,a systematic analysis on Co Cr Fe Ni Mn-0.18 Ti HEA elucidates the microstructural evolution in detail.These findings provide a deep understanding of in situ alloying and pave the way to develop new alloys specific to the EBM process.
基金supported by the National Natural Science Foundation of China,No. 30770754
文摘Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.
基金supported by the National Natural Science Foundation of China,Nos.81501048(to JD),81801236(to ZMX),81974189(to HLT)Shanghai 6th People’s Hospital Research Fund,No.ynlc201808(to JD).
文摘Studies have found that the phosphatase actin regulatory factor 1 expression can be related to stroke,but it remains unclear whether changes in phosphatase actin regulatory factor 1 expression also play a role in traumatic brain injury.In this study we found that,in a mouse model of traumatic brain injury induced by controlled cortical impact,phosphatase actin regulatory factor 1 expression is increased in endothelial cells,neurons,astrocytes,and microglia.When we overexpressed phosphatase actin regulatory factor 1 by injection an adeno-associated virus vector into the contused area in the traumatic brain injury mice,the water content of the brain tissue increased.However,when phosphatase actin regulatory factor 1 was knocked down,the water content decreased.We also found that inhibiting phosphatase actin regulatory factor 1 expression regulated the nuclear factor kappa B signaling pathway,decreased blood-brain barrier permeability,reduced aquaporin 4 and intercellular adhesion molecule 1 expression,inhibited neuroinflammation,and neuronal apoptosis,thereby improving neurological function.The findings from this study indicate that phosphatase actin regulatory factor 1 may be a potential therapeutic target for traumatic brain injury.
基金supports by National Natural Science Foundation of China(grant no.51902265)Fundamental Research Funds for the Central Universities,Key Research and Development Program of Shaanxi(no.2020KWZ-001)Project for graduate Innovation team of Northwestern Polytechnical University.
文摘The increasing energy requirements to power the modern world has driven active research into more advanced electrochemical energy storage devices(EESD)with both high energy densities and power densities.Wide range of newly discovered materials with promising electrochemical properties has shown great potential for next-generation devices,but their performance is normally associated with contradicting demands of thin electrodes and high mass loading that can be hardly achieved for practical applications.Design of three-dimensional(3D)porous electrodes can increase the mass loading while maintaining the effective charge transport even with thick electrodes,which has proven to be efficient to overcome the limitations.3D structures have also been demonstrated excellent structural stability to withstand strong strains and stresses generated during charge/discharge cycle.3D printing,which can fabricate various delicate and complex structural designs,thus offering brand-new opportunities for the rational design and facile construction of next-generation EESDs.The recent developments in 3D printing of next-generation EESDs with high performance are reviewed.Advanced/multiscale electrode structures,such as hierarchically porous structure that can be constructed via high-resolution 3D printing or with post-treatment,are further emphasized.The ability of current 3D printing techniques to fulfill multimaterial printing to fulfill simple packaging will be covered.
