OBTECTIVE:To explore the role of transient receptor potential vaniiloid subetype 1(TRPV1) in the increase of the thermal pain threshold by moxibustion.METHODS:Forty Kunming mice(20 ± 2) g were randomized into con...OBTECTIVE:To explore the role of transient receptor potential vaniiloid subetype 1(TRPV1) in the increase of the thermal pain threshold by moxibustion.METHODS:Forty Kunming mice(20 ± 2) g were randomized into control group,capsaicin group,capsazepine group,moxibustion group and moxibustion + capsazepine(MC) group with 8 mice in each,and 16 C57BL/6 wild-type mice(18 ± 2) g were randomized into wild-type(WT) control group and WT moxibustion group with 8 mice in each,and 14 TRPV1 knockout mice(18 ± 2) g were randomized into knockout(KO) control group and KO moxibustion group with 7 in each.Each mouse in the capsaicin group was subcutaneously injected with the amount of 0.1 mL/10 g into L5 and L6 spinal cords;each mouse in the capsazepine group was intraperitoneally injected with the amount of0.1 mL/10 g.Similarly,each mouse in the moxibustion group was given a suspended moxibustion with specially-made moxa-stick for 20 min on L5 and L6 spinal cords.Each mouse in MC group was intraperitoneally injected with the amount of 0.1 mL/10 g first,then after 15 min was given a suspended moxibustion for 20 min on L5 and L6 spinal cords.Each mouse in WT moxibustion group and KO moxibustion group was given a suspended moxibustion with specially-made moxa-stick for 20 min on L5 and L6 spinal cords.The control group,WT control group and KO control group were of no treatment in any way.After all treatments were completed,the digital-display measurement instrument for thermal pain was used to measure the threshold of thermal pain in each group respectively.RESULTS:Compared with the control group,the thresholds of thermal pain in the moxibustion group and MC group were significantly increased(P <0.01);no significant changes in the thresholds in the capsaicin group and the capsazepine group(P > 0.05);compared with moxibustion group,he threshold of thermal in MC group was obviously decreased(P < 0.01).Compared with WT control group,the threshold of thermal pain in WT moxibustion group was significantly increased(P <0.01);compared with KO control group,no changes in the threshold in KO moxibustion group(P > 0.05).CONCLUSION:TRPV1 participated in the process of increasing the threshold of thermal pain by stimulating L5 and L6 of mice spinal cord with burning mosa-stick.展开更多
与游戏相交织的教学空间杭州师范大学附属湖州鹤和小学位于湖州市太湖旅游度假区震泽路西侧。建制为8规48班,总建筑面积3.17万平方米,其中地上2.9万平方米,地下0.27万平方米。从建筑的外部形态看,鹤和小学的空间是从三、四层明确的围合...与游戏相交织的教学空间杭州师范大学附属湖州鹤和小学位于湖州市太湖旅游度假区震泽路西侧。建制为8规48班,总建筑面积3.17万平方米,其中地上2.9万平方米,地下0.27万平方米。从建筑的外部形态看,鹤和小学的空间是从三、四层明确的围合,到二层的半围合,再到一层的围而不合。从空间的内部结构看,这种空间的组合在立体上建构了一个同时具有广场尺度与庭院尺度、地面活动与屋顶活动、室内与室外、上部与下部相互交织且彼此并存的空间关联。从设计策略上看,正是三、层相对集中的空间使用效率为一、二层大量开放的“非功能空间”在建筑面积上提供了可能的余地。从功能分布上看,三、四楼是明确的教室空间以满足基本的日常教学,到了二层,户外公共活动活动平台明显增加,并以台阶与坡道的方式与一楼的院落和东侧的运动场广泛连接。而一楼,8 WORLD ARCHITECTURE REVIEW本期人物PERSONAGE OF THE EDITION少量围合的文体类空间也是明显地倾向于素质教育,并“与室外空间直接连接”,“鼓励偶然交流”并强调“空间的适应性与可塑性”。从材料与色彩上看,竹模混凝土及竹制品的应用让建筑有了明显在地性的文化属性,而明显是与非功能空间相结合的色彩应用,则是在视觉上进一步完善与补充了空间功能的定义并表述为明确的行为引导。展开更多
基金Supported by National Key Basic Research Program 973(Dual Effects of Acupuncture on Functional Intestinal Disease and Its Relationship with Autonomic Nervous Function,No.2011cb505206)2013 Jiangsu Province Education Department of Natural Science Research of Major Projects(Research on The Role of Trpv1 About Anti-inflammation And Analgesia Effect of Moxibustion Treatment,No.13kja360001)Academic Propagate Project on Scientific And Technical Innovation Team,Nanjing University Of Chinese Medicine 2013 Scientific And Technical Innovation Team Project
文摘OBTECTIVE:To explore the role of transient receptor potential vaniiloid subetype 1(TRPV1) in the increase of the thermal pain threshold by moxibustion.METHODS:Forty Kunming mice(20 ± 2) g were randomized into control group,capsaicin group,capsazepine group,moxibustion group and moxibustion + capsazepine(MC) group with 8 mice in each,and 16 C57BL/6 wild-type mice(18 ± 2) g were randomized into wild-type(WT) control group and WT moxibustion group with 8 mice in each,and 14 TRPV1 knockout mice(18 ± 2) g were randomized into knockout(KO) control group and KO moxibustion group with 7 in each.Each mouse in the capsaicin group was subcutaneously injected with the amount of 0.1 mL/10 g into L5 and L6 spinal cords;each mouse in the capsazepine group was intraperitoneally injected with the amount of0.1 mL/10 g.Similarly,each mouse in the moxibustion group was given a suspended moxibustion with specially-made moxa-stick for 20 min on L5 and L6 spinal cords.Each mouse in MC group was intraperitoneally injected with the amount of 0.1 mL/10 g first,then after 15 min was given a suspended moxibustion for 20 min on L5 and L6 spinal cords.Each mouse in WT moxibustion group and KO moxibustion group was given a suspended moxibustion with specially-made moxa-stick for 20 min on L5 and L6 spinal cords.The control group,WT control group and KO control group were of no treatment in any way.After all treatments were completed,the digital-display measurement instrument for thermal pain was used to measure the threshold of thermal pain in each group respectively.RESULTS:Compared with the control group,the thresholds of thermal pain in the moxibustion group and MC group were significantly increased(P <0.01);no significant changes in the thresholds in the capsaicin group and the capsazepine group(P > 0.05);compared with moxibustion group,he threshold of thermal in MC group was obviously decreased(P < 0.01).Compared with WT control group,the threshold of thermal pain in WT moxibustion group was significantly increased(P <0.01);compared with KO control group,no changes in the threshold in KO moxibustion group(P > 0.05).CONCLUSION:TRPV1 participated in the process of increasing the threshold of thermal pain by stimulating L5 and L6 of mice spinal cord with burning mosa-stick.
文摘与游戏相交织的教学空间杭州师范大学附属湖州鹤和小学位于湖州市太湖旅游度假区震泽路西侧。建制为8规48班,总建筑面积3.17万平方米,其中地上2.9万平方米,地下0.27万平方米。从建筑的外部形态看,鹤和小学的空间是从三、四层明确的围合,到二层的半围合,再到一层的围而不合。从空间的内部结构看,这种空间的组合在立体上建构了一个同时具有广场尺度与庭院尺度、地面活动与屋顶活动、室内与室外、上部与下部相互交织且彼此并存的空间关联。从设计策略上看,正是三、层相对集中的空间使用效率为一、二层大量开放的“非功能空间”在建筑面积上提供了可能的余地。从功能分布上看,三、四楼是明确的教室空间以满足基本的日常教学,到了二层,户外公共活动活动平台明显增加,并以台阶与坡道的方式与一楼的院落和东侧的运动场广泛连接。而一楼,8 WORLD ARCHITECTURE REVIEW本期人物PERSONAGE OF THE EDITION少量围合的文体类空间也是明显地倾向于素质教育,并“与室外空间直接连接”,“鼓励偶然交流”并强调“空间的适应性与可塑性”。从材料与色彩上看,竹模混凝土及竹制品的应用让建筑有了明显在地性的文化属性,而明显是与非功能空间相结合的色彩应用,则是在视觉上进一步完善与补充了空间功能的定义并表述为明确的行为引导。
