目的总结黄芪多糖的提取制备技术、质量控制方法和免疫调控作用研究进展。方法检索维普、中国知网、Web of Science及PubMed数据库自建库起至2024年3月31日与黄芪多糖相关的文献,总结黄芪多糖的提取制备技术、质量控制及免疫调控的研究...目的总结黄芪多糖的提取制备技术、质量控制方法和免疫调控作用研究进展。方法检索维普、中国知网、Web of Science及PubMed数据库自建库起至2024年3月31日与黄芪多糖相关的文献,总结黄芪多糖的提取制备技术、质量控制及免疫调控的研究现状,并提出发展建议。结果不同提取方法对黄芪多糖的含量、纯度均有不同的影响,其中酶辅助提取法的提取效率最高。黄芪多糖化学结构复杂,需进一步优化多糖结构分析技术,建立统一的质控标准。黄芪多糖能通过调节免疫器官、免疫细胞、细胞因子等发挥免疫调控作用,从而产生抗病毒、抗肿瘤等功效。结论未来需进一步加强黄芪多糖的结构与功能研究,并深入阐明其药效机制,为进一步研究黄芪多糖的制备、质量控制、免疫调控作用机制及临床应用提供参考。展开更多
目的为新型程序性死亡受体-1(PD-1)/程序性死亡配体-1(PD-L1)小分子抑制剂的研发提供参考。方法检索PubMed、Embase、Web of Science、ClinicalTrails.gov、中国知网、万方数据库2010年至2023年的PD-1/PD-L1小分子抑制剂相关文献,汇总...目的为新型程序性死亡受体-1(PD-1)/程序性死亡配体-1(PD-L1)小分子抑制剂的研发提供参考。方法检索PubMed、Embase、Web of Science、ClinicalTrails.gov、中国知网、万方数据库2010年至2023年的PD-1/PD-L1小分子抑制剂相关文献,汇总并分析该类制剂的研发现状。结果与结论有成药潜力的PD-1/PD-L1小分子抑制剂共20种,包括CA-170(口服小分子抑制剂)、INCB086550(特异性PD-L1抑制剂)、DPPA-1(特异性抑制PD-1/PD-L1相互作用的多肽类拮抗剂)等,其中前两者已进入临床试验阶段。PD-1/PD-L1小分子抑制剂具有特异性抑制免疫检查点的药效作用特点,以及可口服、稳定性较好、膜通透性较高等优点,但其治疗效果仍需临床试验验证。展开更多
Long-term fluorescence monitoring of subcellular organelles is crucial for cellular physiology and pathology studies.Lipid droplets(LDs)are increasingly recognized for their involvement in various biological processes...Long-term fluorescence monitoring of subcellular organelles is crucial for cellular physiology and pathology studies.Lipid droplets(LDs)are increasingly recognized for their involvement in various biological processes,to influence disease development through diverse behaviors However,existing LD probes face challenges in achieving high targeting and long-term monitoring due to poor photostability and long-term phototoxicity.Carbon quantum dots(CQDs)have gained prominence due to their exceptional fluorescence properties,but their prevalent blue excitation wavelength presents difficulties for long-term imaging.Herein,we synthesized red-emissive carbon quantum dot(R-CQDs)with superior photobleaching resistance and red-emission,thus enabling harmlessly fluorescence monitoring of cells longer than3 h.In addition,R-CQD exhibits suitable amphiphilicity and remarkable solvatochromic effect,allowing rapid targeting to LDs for immediate imaging without cumbersome washing steps.Hence,R-CQD shows high performance for extended observation of dynamic LD behavior in various biological processes,which is confirmed by documenting the course of LDs during starvation as well as lipotoxicity.Compared to commercial probes,R-CQD extends live cell imaging time by at least 9-fold,facilitating the study of LD behavioral characteristics under diverse physiological or pathological conditions.This work provides a reliable fluorescence tool for tracking intercellular microenvironment dynamically thus to understand the divers biological or disease mechanism.展开更多
文摘目的总结黄芪多糖的提取制备技术、质量控制方法和免疫调控作用研究进展。方法检索维普、中国知网、Web of Science及PubMed数据库自建库起至2024年3月31日与黄芪多糖相关的文献,总结黄芪多糖的提取制备技术、质量控制及免疫调控的研究现状,并提出发展建议。结果不同提取方法对黄芪多糖的含量、纯度均有不同的影响,其中酶辅助提取法的提取效率最高。黄芪多糖化学结构复杂,需进一步优化多糖结构分析技术,建立统一的质控标准。黄芪多糖能通过调节免疫器官、免疫细胞、细胞因子等发挥免疫调控作用,从而产生抗病毒、抗肿瘤等功效。结论未来需进一步加强黄芪多糖的结构与功能研究,并深入阐明其药效机制,为进一步研究黄芪多糖的制备、质量控制、免疫调控作用机制及临床应用提供参考。
文摘目的为新型程序性死亡受体-1(PD-1)/程序性死亡配体-1(PD-L1)小分子抑制剂的研发提供参考。方法检索PubMed、Embase、Web of Science、ClinicalTrails.gov、中国知网、万方数据库2010年至2023年的PD-1/PD-L1小分子抑制剂相关文献,汇总并分析该类制剂的研发现状。结果与结论有成药潜力的PD-1/PD-L1小分子抑制剂共20种,包括CA-170(口服小分子抑制剂)、INCB086550(特异性PD-L1抑制剂)、DPPA-1(特异性抑制PD-1/PD-L1相互作用的多肽类拮抗剂)等,其中前两者已进入临床试验阶段。PD-1/PD-L1小分子抑制剂具有特异性抑制免疫检查点的药效作用特点,以及可口服、稳定性较好、膜通透性较高等优点,但其治疗效果仍需临床试验验证。
基金supported by the National Natural Science Foundation of China(Nos.52003178,52273141 and 51973132)Natural Science Foundation of Sichuan Province(No.2023NSFSC0338)。
文摘Long-term fluorescence monitoring of subcellular organelles is crucial for cellular physiology and pathology studies.Lipid droplets(LDs)are increasingly recognized for their involvement in various biological processes,to influence disease development through diverse behaviors However,existing LD probes face challenges in achieving high targeting and long-term monitoring due to poor photostability and long-term phototoxicity.Carbon quantum dots(CQDs)have gained prominence due to their exceptional fluorescence properties,but their prevalent blue excitation wavelength presents difficulties for long-term imaging.Herein,we synthesized red-emissive carbon quantum dot(R-CQDs)with superior photobleaching resistance and red-emission,thus enabling harmlessly fluorescence monitoring of cells longer than3 h.In addition,R-CQD exhibits suitable amphiphilicity and remarkable solvatochromic effect,allowing rapid targeting to LDs for immediate imaging without cumbersome washing steps.Hence,R-CQD shows high performance for extended observation of dynamic LD behavior in various biological processes,which is confirmed by documenting the course of LDs during starvation as well as lipotoxicity.Compared to commercial probes,R-CQD extends live cell imaging time by at least 9-fold,facilitating the study of LD behavioral characteristics under diverse physiological or pathological conditions.This work provides a reliable fluorescence tool for tracking intercellular microenvironment dynamically thus to understand the divers biological or disease mechanism.
