OBJECTIVE:To examine the differences in cognitive processing between patients with mild cognitive impairment(MCI)of different Traditional Chinese Medicine(TCM)syndrome types to provide evidence supporting the TCM typi...OBJECTIVE:To examine the differences in cognitive processing between patients with mild cognitive impairment(MCI)of different Traditional Chinese Medicine(TCM)syndrome types to provide evidence supporting the TCM typing of MCI.METHODS:Participants were screened using a battery of scales for spleen and kidney deficiency(SKD)/liver Qi stagnation(LQS)-type MCI or those without syndrome or normal control(NC).Following sex,age,and educational matching,behavioral and electroencephalographic data were recorded using the verbal N-back experimental paradigm.The data were then analyzed and compared with respect to the reaction time and correctness of the participants in each group,as well as the amplitude and latency of the event-related potential(ERP)components of P2,N2,and P3.RESULTS:There were no statistically significant differences in the accuracy or reaction times of the behavioral data of the groups.Regarding ERP data,the SKD group had a shorter P2 latency than the LQS and NC groups,while the latter two groups did not differ statistically.The SKD group had a shorter N2 latency than the NC group,while the SKD group did not differ from the LQS group.The SKD and LQS groups had a shorter P3 latency than the NC group.CONCLUSION:Our study offers objective evidence of the distinction between the types of TCM syndrome.Different types of TCM syndromes produce different disease mechanisms,resulting in brain damage with different presentations of cognitive impairment and cognitive processing characteristics.展开更多
目的探究五行音乐对孕期恐应激子代抑郁样行为和肠道菌群的影响。方法将36只孕0.5 d Wistar雌鼠随机分为空白组、恐应激组和五行音乐组,所产仔鼠延续母代分组。孕1~19 d恐应激组和五行音乐组孕鼠采用旁观电击法造模,同时五行音乐组予五...目的探究五行音乐对孕期恐应激子代抑郁样行为和肠道菌群的影响。方法将36只孕0.5 d Wistar雌鼠随机分为空白组、恐应激组和五行音乐组,所产仔鼠延续母代分组。孕1~19 d恐应激组和五行音乐组孕鼠采用旁观电击法造模,同时五行音乐组予五行音乐羽调干预。采用旷场实验、酶联免疫吸附法(ELISA)检测孕20 d孕鼠恐惧行为及血清糖皮质激素(glucocorticoid,GC)含量评价造模效果;采用旷场、悬尾和糖水偏好实验评价3周龄仔鼠抑郁情绪;采用高效液相检测仔鼠海马神经递质去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)和5-羟色胺(5-hydroxytryptamine,5-HT)水平;采用16S rRNA测序分析仔鼠肠道菌群变化规律;采用蛋白质免疫印迹(Western blot)实验检测仔鼠近端结肠蛋白Claudin1、Occludin和ZO1的表达。结果与空白组相比,恐应激组孕鼠旷场周围区域停留时间延长(P<0.05),进入中心区域次数减少(P<0.001),血清GC水平升高(P<0.001),而五行音乐组逆转了恐应激组的行为学变化(P<0.05)及血清GC水平(P<0.001)。相较空白组,恐应激组仔鼠旷场穿格次数减少(P<0.01)、糖水偏好指数降低、悬尾不动时间延长(P<0.05),海马内NE、DA、5-HT显著减少(P<0.05),肠道菌群多样性下降(P<0.01),菌群结构显著改变,其中变形菌门、肠杆菌科、肠球菌属、埃希氏菌属等丰度上调(P<0.05),螺旋体门、螺旋体科、毛螺菌科、瘤胃球菌科、密螺旋体属、普雷沃氏菌属、粪球菌属、异杆菌属、瘤胃球菌属、多尔氏菌属等丰度下调(P<0.05),近端结肠蛋白Claudin1、Occludin和ZO1表达量显著下调(P<0.05);相较恐应激组,五行音乐组仔鼠穿格次数、糖水偏好指数回升、悬尾不动时间缩短(P<0.05),海马内DA、5-HT显著回升(P<0.05),肠道菌群物种多样性增加(P<0.01),且逆转了上述菌群丰度变化,近端结肠蛋白Occludin和ZO1表达量显著上调(P<0.05)。结论孕期五行音乐干预能有效改善孕期恐应激所致子代抑郁样行为及肠道菌群紊乱。展开更多
目的采用基于低频振幅(amplitude of low frequency fluctuation,ALFF)算法的静息态功能磁共振成像(function-al magnetic resonance imaging,fMRI)技术探讨轻度认知功能障碍(mild cognitive impairment,MCI)的客观诊断指标及疏肝法改...目的采用基于低频振幅(amplitude of low frequency fluctuation,ALFF)算法的静息态功能磁共振成像(function-al magnetic resonance imaging,fMRI)技术探讨轻度认知功能障碍(mild cognitive impairment,MCI)的客观诊断指标及疏肝法改善肝郁型MCI患者认知功能的机制。方法以肝郁型MCI患者10例为病例组,年龄、性别、文化程度与之匹配的正常人10例被视为对照组,采集病例组用药前后及对照组静息态fMRI的血氧水平依赖(blood oxygenation level dependent,BOLD)信号,分析ALFF值的差异。结果与对照组相比,病例组ALFF值降低的区域主要位于左侧枕中回和岛盖部额下回(P<0.01,AlphaSim校正),ALFF值增高的区域为右内侧前额叶(P<0.01,AlphaSim校正)。与用药前相比,病例组用药后ALFF值降低的区域主要位于右侧枕中回(P<0.01,AlphaSim校正),ALFF值增高的区域主要集中在左侧颞上回和左侧颞中回(P<0.01,AlphaSim校正)。结论ALFF值有望成为诊断MCI的客观指标,左侧颞上回和左侧颞中回兴奋性增高是疏肝法改善肝郁型MCI患者认知功能的可能机制。展开更多
基金Supported by National Natural Science Foundation of China-funded Project:Study on Mechanism of“Smoothing The Liver Therapy”on Working Memory of the Patients with Mild Cognitive Impairment Caused by Negative Emotion Regulation(No.81473556)Effects and Mechanism of Liver’s Failing to Facilitate the Coursing of Qi on Decline Process of Cognitive Function of Normal People and Patients with MCI(No.81873208)。
文摘OBJECTIVE:To examine the differences in cognitive processing between patients with mild cognitive impairment(MCI)of different Traditional Chinese Medicine(TCM)syndrome types to provide evidence supporting the TCM typing of MCI.METHODS:Participants were screened using a battery of scales for spleen and kidney deficiency(SKD)/liver Qi stagnation(LQS)-type MCI or those without syndrome or normal control(NC).Following sex,age,and educational matching,behavioral and electroencephalographic data were recorded using the verbal N-back experimental paradigm.The data were then analyzed and compared with respect to the reaction time and correctness of the participants in each group,as well as the amplitude and latency of the event-related potential(ERP)components of P2,N2,and P3.RESULTS:There were no statistically significant differences in the accuracy or reaction times of the behavioral data of the groups.Regarding ERP data,the SKD group had a shorter P2 latency than the LQS and NC groups,while the latter two groups did not differ statistically.The SKD group had a shorter N2 latency than the NC group,while the SKD group did not differ from the LQS group.The SKD and LQS groups had a shorter P3 latency than the NC group.CONCLUSION:Our study offers objective evidence of the distinction between the types of TCM syndrome.Different types of TCM syndromes produce different disease mechanisms,resulting in brain damage with different presentations of cognitive impairment and cognitive processing characteristics.
文摘目的采用基于低频振幅(amplitude of low frequency fluctuation,ALFF)算法的静息态功能磁共振成像(function-al magnetic resonance imaging,fMRI)技术探讨轻度认知功能障碍(mild cognitive impairment,MCI)的客观诊断指标及疏肝法改善肝郁型MCI患者认知功能的机制。方法以肝郁型MCI患者10例为病例组,年龄、性别、文化程度与之匹配的正常人10例被视为对照组,采集病例组用药前后及对照组静息态fMRI的血氧水平依赖(blood oxygenation level dependent,BOLD)信号,分析ALFF值的差异。结果与对照组相比,病例组ALFF值降低的区域主要位于左侧枕中回和岛盖部额下回(P<0.01,AlphaSim校正),ALFF值增高的区域为右内侧前额叶(P<0.01,AlphaSim校正)。与用药前相比,病例组用药后ALFF值降低的区域主要位于右侧枕中回(P<0.01,AlphaSim校正),ALFF值增高的区域主要集中在左侧颞上回和左侧颞中回(P<0.01,AlphaSim校正)。结论ALFF值有望成为诊断MCI的客观指标,左侧颞上回和左侧颞中回兴奋性增高是疏肝法改善肝郁型MCI患者认知功能的可能机制。
