Banana(Musa spp.)is an ancient and popular fruit plant with highly nutritious fruit.The pseudo-stem of banana represents on average 75%of the total dry mass but its valorization as a nutritional and industrial by-prod...Banana(Musa spp.)is an ancient and popular fruit plant with highly nutritious fruit.The pseudo-stem of banana represents on average 75%of the total dry mass but its valorization as a nutritional and industrial by-product is limited.Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts.This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem.Hereby,we identified and quantified 373 metabolites from a diverse range of classes including,alkaloids,flavonoids,lipids,phenolic acids,amino acids and its derivatives,nucleotide and its derivatives,organic acids,lignans and coumarins,tannins,and terpene using the widely-targeted metabolomics approach.Banana pseudo-stem is enriched in metabolites for utilization in the food industry(L-lysine and L-tryptophan,L-glutamic acid,Phenylalanine,Palmitoleic acid,α-Linolenic acid,and Lauric acid,and Adenine)and pharmaceutical industry(Guanosine and Cimidahurinine,Bergapten,Coumarins,Procyanidin A2,Procyanidin B1,Procyanidin B3,Procyanidin B2,and Procyanidin B4,Asiatic acid).The metabolome of banana pseudo-stem with integration across multiomics data may provide the opportunity to exploit the rich metabolome of banana pseudo-stem for industrial and nutritional applications.展开更多
Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al....Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al.,2025).However,its rapid softening severely limits shelf life,causing substantial economic losses during transport and storage.Recently,the enhanced shelf-life can be generated by compromising the key ripening regulators,such as RIN,but other fruit quality traits including flavor and color also can be impaired concurrently(Kitagawa et al.,2005).展开更多
The Streptococcus-derived CRISPR/Cas9 system can introduce precise and predictable modifications into the plant genome to obtain the desired traits.As one of the most advanced tools for editing crop genomes,the CRISPR...The Streptococcus-derived CRISPR/Cas9 system can introduce precise and predictable modifications into the plant genome to obtain the desired traits.As one of the most advanced tools for editing crop genomes,the CRISPR/Cas9 system has been expanding rapidly and has been widely applied to determine gene function and improve agronomic traits in horticultural crops such as fruits and vegetables(Ma et al.2023).展开更多
Bananas(Musa spp.)are one of the world’s most important fruit crops and play a vital role in food security for many developing countries.Most banana cultivars are triploids derived from inter-and intraspecific hybrid...Bananas(Musa spp.)are one of the world’s most important fruit crops and play a vital role in food security for many developing countries.Most banana cultivars are triploids derived from inter-and intraspecific hybrid-izations between the wild diploid ancestor species Musa acuminate(AA)and M.balbisiana(BB).We report two haplotype-resolved genome assemblies of the representative AAB-cultivated types,Plantain and Silk,and precisely characterize ancestral contributions by examining ancestry mosaics across the genome.Widespread asymmetric evolution is observed in their subgenomes,which can be linked to frequent homol-ogous exchange events.We reveal the genetic makeup of triploid banana cultivars and verify that subge-nome B is a rich source of disease resistance genes.Only 58.5%and 59.4%of Plantain and Silk genes,respectively,are present in all three haplotypes,with>50%of genes being differentially expressed alleles in different subgenomes.We observed that the number of upregulated genes in Plantain is significantly higher than that in Silk at one-week post-inoculation with Fusarium wilt tropical race 4(Foc TR4),which con-firms that Plantain can initiate defense responses faster than Silk.Additionally,we compared genomic and transcriptomic differences among the genes related to carotenoid synthesis and starch metabolism between Plantain and Silk.Our study provides resources for better understanding the genomic architecture of culti-vated bananas and has important implications for Musa genetics and breeding.展开更多
Bananas(Musa spp.)are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical countries.The fruits are a popular food,and the plants themselves...Bananas(Musa spp.)are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical countries.The fruits are a popular food,and the plants themselves have diverse uses.Four genetic groups(genomes)are thought to have contributed to current banana cultivars:Musa acuminata(A genome),Musa balbisiana(B genome),Musa schizocarpa(S genome),and species of the Australimusa section(T genome).However,the T genome has not been effectively explored.Here,we present the high-quality TT genomes of two representative accessions,Abaca(Musa textilis),with high-quality naturalfiber,and Utafun(Musa troglodytarum,Fe’i group),with abundant b-carotene.Both the Abaca and Utafun assemblies comprise 10 pseudochromosomes,and their total genome sizes are 613 Mb and 619 Mb,respectively.Comparative genome analysis revealed that the larger size of the T genome is likely attributable to rapid expansion and slow removal of trans-posons.Compared with those of Musa AA or BB accessions or sisal(Agava sisalana),Abacafibers exhibit superior mechanical properties,mainly because of their thicker cell walls with a higher content of cellulose,lignin,and hemicellulose.Expression of MusaCesA cellulose synthesis genes peaks earlier in Abaca than in AA or BB accessions during plant development,potentially leading to earlier cellulose accumulation during secondary cell wall formation.The Abaca-specific expressed gene MusaMYB26,which is directly regulated by MusaMYB61,may be an important regulator that promotes precocious expression of secondary cell wall MusaCesAs.Furthermore,MusaWRKY2 and MusaNAC68,which appear to be involved in regulating expression of MusaLAC and MusaCAD,may at least partially explain the high accumulation of lignin in Abaca.This work contributes to a better understanding of banana domestica-tion and the diverse genetic resources in the Musaceae family,thus providing resources for Musa genetic improvement.展开更多
Since publication of a draft genome of the doubled-haploid‘Pahang’banana(Musa acuminata,DH-Pahang),a new era for banana biology research has begun.With the release of genomic data from some important Musa species an...Since publication of a draft genome of the doubled-haploid‘Pahang’banana(Musa acuminata,DH-Pahang),a new era for banana biology research has begun.With the release of genomic data from some important Musa species and subspecies and the continuous development of molecular biology techniques,significant progress has been made.Here,we summarize the achievements and advances in the banana molecular biology and breeding over the past decade covering origin and domestication,fruit biology,stress biology,and breeding aspects,and highlight their challenges and future perspectives.This review is intended to provide researchers with the latest information on the complex genetic background and evolutionary relationship of bananas,the biology of fruit ripening,and multi-omics-based stress biology research.We especially focus on recent advances in the molecular breeding of bananas,offering an informative research direction and providing valuable technical references for future research in the field.展开更多
基金This research was financially supported by National Key Research and Development Project(2018YFD1000102,2019YFD1000200,2019YFD1000901)Guangdong Science and Technology Project(2019B030316007)+2 种基金special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science(R2018PY-QY004,R2017PY-QY001,R2017PY-JX002)Guangzhou national modern agricultural industry science and technology innovation center project(2018kczx06)National Banana Industry and Technology System Project(CARS-31-01).
文摘Banana(Musa spp.)is an ancient and popular fruit plant with highly nutritious fruit.The pseudo-stem of banana represents on average 75%of the total dry mass but its valorization as a nutritional and industrial by-product is limited.Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts.This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem.Hereby,we identified and quantified 373 metabolites from a diverse range of classes including,alkaloids,flavonoids,lipids,phenolic acids,amino acids and its derivatives,nucleotide and its derivatives,organic acids,lignans and coumarins,tannins,and terpene using the widely-targeted metabolomics approach.Banana pseudo-stem is enriched in metabolites for utilization in the food industry(L-lysine and L-tryptophan,L-glutamic acid,Phenylalanine,Palmitoleic acid,α-Linolenic acid,and Lauric acid,and Adenine)and pharmaceutical industry(Guanosine and Cimidahurinine,Bergapten,Coumarins,Procyanidin A2,Procyanidin B1,Procyanidin B3,Procyanidin B2,and Procyanidin B4,Asiatic acid).The metabolome of banana pseudo-stem with integration across multiomics data may provide the opportunity to exploit the rich metabolome of banana pseudo-stem for industrial and nutritional applications.
基金supported by the Natural Science Foundation of China(Grant Nos.32172544,32441071)the special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science(Grant No.R2023PY-JG003)+3 种基金the earmarked fund for CARS(Grant No.CARS-31-01)Guangdong Special Support Program(Grant No.NYLJ2024010)Guangdong S&T Program(Grant No.2025B0202070005)IAEA CRP D23033,and the Project from Guangzhou Municipal Science and Technology Bureau(2023B03J0991).
文摘Banana(Musa spp.)is one of the most important fruit crop worldwide,and plays a critical role in human diet and agricultural economies across tropical and subtropical regions,including China(Jiang et al.,2025;Wu et al.,2025).However,its rapid softening severely limits shelf life,causing substantial economic losses during transport and storage.Recently,the enhanced shelf-life can be generated by compromising the key ripening regulators,such as RIN,but other fruit quality traits including flavor and color also can be impaired concurrently(Kitagawa et al.,2005).
基金Open access funding provided by Shanghai Jiao Tong Universitysupported by grants from the National Key R&D Project(2019YFD1000900)+4 种基金a Project from Guangzhou Municipal Science and Technology Bureau(201904020033 and 2023B03J0991)the Natural Science Foundation of China(31772289)Laboratory of Lingnan Modern Agriculture Project(NT2021004,2021TDQD003)supported by the earmarked fund for CARS(CARS-31)funded by the Key Realm R&D Program of Guangdong Province(2020B0202090005).
文摘The Streptococcus-derived CRISPR/Cas9 system can introduce precise and predictable modifications into the plant genome to obtain the desired traits.As one of the most advanced tools for editing crop genomes,the CRISPR/Cas9 system has been expanding rapidly and has been widely applied to determine gene function and improve agronomic traits in horticultural crops such as fruits and vegetables(Ma et al.2023).
基金funded by the Strategy of Rural Vitalization of Guangdong Provinces (2022-NPY-00-003,2022-NJS-00-001)the National Natural Science Foundation of China (32270712)+4 种基金the earmarked fund for CARS (CARS-31-01)GDAAS (202102TD,R2020PY-JX002)the Ba-Gui Scholar Program of Guangxi (to Z.-G.H)the Laboratory of Lingnan Modern Agriculture Project (NT2021004)the Maoming Branch Grant (2021TDQD003).
文摘Bananas(Musa spp.)are one of the world’s most important fruit crops and play a vital role in food security for many developing countries.Most banana cultivars are triploids derived from inter-and intraspecific hybrid-izations between the wild diploid ancestor species Musa acuminate(AA)and M.balbisiana(BB).We report two haplotype-resolved genome assemblies of the representative AAB-cultivated types,Plantain and Silk,and precisely characterize ancestral contributions by examining ancestry mosaics across the genome.Widespread asymmetric evolution is observed in their subgenomes,which can be linked to frequent homol-ogous exchange events.We reveal the genetic makeup of triploid banana cultivars and verify that subge-nome B is a rich source of disease resistance genes.Only 58.5%and 59.4%of Plantain and Silk genes,respectively,are present in all three haplotypes,with>50%of genes being differentially expressed alleles in different subgenomes.We observed that the number of upregulated genes in Plantain is significantly higher than that in Silk at one-week post-inoculation with Fusarium wilt tropical race 4(Foc TR4),which con-firms that Plantain can initiate defense responses faster than Silk.Additionally,we compared genomic and transcriptomic differences among the genes related to carotenoid synthesis and starch metabolism between Plantain and Silk.Our study provides resources for better understanding the genomic architecture of culti-vated bananas and has important implications for Musa genetics and breeding.
基金funded by the National Key R&D Program of China (2019YFD1000203 and 2019YFD1000900)the National Natural Science Foundation of China (32270712)+3 种基金the earmarked fund for CARS (CARS-31-01)GDAAS (202102TD,and R2020PY-JX002)funds for the strategy of rural vitalization of Guangdong province,a Laboratory of Lingnan Modern Agriculture Project (NT2021004)a Maoming Branch grant (2021TDQD003).
文摘Bananas(Musa spp.)are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical countries.The fruits are a popular food,and the plants themselves have diverse uses.Four genetic groups(genomes)are thought to have contributed to current banana cultivars:Musa acuminata(A genome),Musa balbisiana(B genome),Musa schizocarpa(S genome),and species of the Australimusa section(T genome).However,the T genome has not been effectively explored.Here,we present the high-quality TT genomes of two representative accessions,Abaca(Musa textilis),with high-quality naturalfiber,and Utafun(Musa troglodytarum,Fe’i group),with abundant b-carotene.Both the Abaca and Utafun assemblies comprise 10 pseudochromosomes,and their total genome sizes are 613 Mb and 619 Mb,respectively.Comparative genome analysis revealed that the larger size of the T genome is likely attributable to rapid expansion and slow removal of trans-posons.Compared with those of Musa AA or BB accessions or sisal(Agava sisalana),Abacafibers exhibit superior mechanical properties,mainly because of their thicker cell walls with a higher content of cellulose,lignin,and hemicellulose.Expression of MusaCesA cellulose synthesis genes peaks earlier in Abaca than in AA or BB accessions during plant development,potentially leading to earlier cellulose accumulation during secondary cell wall formation.The Abaca-specific expressed gene MusaMYB26,which is directly regulated by MusaMYB61,may be an important regulator that promotes precocious expression of secondary cell wall MusaCesAs.Furthermore,MusaWRKY2 and MusaNAC68,which appear to be involved in regulating expression of MusaLAC and MusaCAD,may at least partially explain the high accumulation of lignin in Abaca.This work contributes to a better understanding of banana domestica-tion and the diverse genetic resources in the Musaceae family,thus providing resources for Musa genetic improvement.
基金supported by the Research Fund of Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture(2021TDQD003)the Laboratory of Lingnan Modern Agriculture Project(NT2021004)+3 种基金the Young and Middle-aged Discipline Leader“Jin Ying light”Training Program of Guangdong Academy of Agricultural Sciences(R2023PY-JG004)Outstanding Team Project of Guangdong Academy of Agricultural Sciences(202102TD)the Modern Agricultural Innovation Team Project of Guangdong Province(2023KJ106 and 2022KJ106)the Banana Seed Industry Innovation Park Project of the Guangdong Provincial Department of Agriculture and Rural Affairs(2022-NJS-00-001).
文摘Since publication of a draft genome of the doubled-haploid‘Pahang’banana(Musa acuminata,DH-Pahang),a new era for banana biology research has begun.With the release of genomic data from some important Musa species and subspecies and the continuous development of molecular biology techniques,significant progress has been made.Here,we summarize the achievements and advances in the banana molecular biology and breeding over the past decade covering origin and domestication,fruit biology,stress biology,and breeding aspects,and highlight their challenges and future perspectives.This review is intended to provide researchers with the latest information on the complex genetic background and evolutionary relationship of bananas,the biology of fruit ripening,and multi-omics-based stress biology research.We especially focus on recent advances in the molecular breeding of bananas,offering an informative research direction and providing valuable technical references for future research in the field.
