The clinical benefit of combination therapy is significant,but it is not easy to define the mechanism of complexity and diversity.Previous studies illustrate that phillygenin(Phi)binds in the allosteric inhibit pocket...The clinical benefit of combination therapy is significant,but it is not easy to define the mechanism of complexity and diversity.Previous studies illustrate that phillygenin(Phi)binds in the allosteric inhibit pocket of protein kinase B(AKT),and swertiamarin(Swe)acts on the pleckstrin homology(PH)domain of AKT.However,the combined synergistic effect of relieving the inflammatory response has yet to be elucidated.Based on high sensitivity,specificity and fast-responsibility fluorescent sensors,the Förster resonance energy transfer(FRET)technique offers a route to provide clear insights into physiological and pathological processes.In the study,molecular docking,the fluorescent probes of Phi and Swe for FRET were designed and synthesized.FRET analysis shown that Swe and Phi concurrently acted on the PH domain and allosterically inhibited pocket of AKT,respectively.The combination of Swe and Phi significantly increased the heat stability of AKT and decreased protease-induced degeneration.In lipopolysaccharides(LPS)-induced mice and cells,the combination arrested AKT activation,nuclear factor kappa-B(NF-κB)phosphorylation,and the expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-8.In conclusion,FRET revealed Phi and Swe concurrently targeted AKT on different domains and the combination of Phi and Swe enhanced the anti-inflammatory effect.展开更多
目的了解“互联网+”教学模式在中医护理教学中的应用现状及效果,为提高中医护理信息化教学提供参考。方法系统检索PubMed、Web of Science、Cochrane Library、Embase、中国知网、万方数据知识服务平台、维普数据库、中国生物医学文献...目的了解“互联网+”教学模式在中医护理教学中的应用现状及效果,为提高中医护理信息化教学提供参考。方法系统检索PubMed、Web of Science、Cochrane Library、Embase、中国知网、万方数据知识服务平台、维普数据库、中国生物医学文献数据库中“互联网+”教学模式在中医护理教学中应用的相关文献,检索时限为建库至2024年9月30日,对纳入的文献进行资料汇总与分析。结果共纳入19篇文献,教学方式多采用线上+线下混合教学模式;评价指标多样但无统一标准,除课程成绩外,还包括自主学习能力、批判性思维等指标。结论“互联网+”教学模式虽在中医护理教学中应用广泛,但实践过程中存在教学方式和评价体系有待优化的问题,后续需进一步完善。展开更多
以CoO和Pt催化剂(Co-Pt催化剂)作为二元催化剂并将其应用到锂空气电池中,通过场发射扫描电镜和X射线粉末衍射进行物理表征,采用循环伏安、交流阻抗和恒流充放电法研究样品的电化学性能。结果表明,Co-Pt二元催化剂具有良好的电化学性能,...以CoO和Pt催化剂(Co-Pt催化剂)作为二元催化剂并将其应用到锂空气电池中,通过场发射扫描电镜和X射线粉末衍射进行物理表征,采用循环伏安、交流阻抗和恒流充放电法研究样品的电化学性能。结果表明,Co-Pt二元催化剂具有良好的电化学性能,在0.025×10–3A·cm^(–2)的电流密度下首次放电比容量和能量密度分别可达到2225.8 m Ah·g^(–1)和5822.8 m Wh·g^(–1)。展开更多
基金supported by the National Natural Science Foundation of China(No.81973449).
文摘The clinical benefit of combination therapy is significant,but it is not easy to define the mechanism of complexity and diversity.Previous studies illustrate that phillygenin(Phi)binds in the allosteric inhibit pocket of protein kinase B(AKT),and swertiamarin(Swe)acts on the pleckstrin homology(PH)domain of AKT.However,the combined synergistic effect of relieving the inflammatory response has yet to be elucidated.Based on high sensitivity,specificity and fast-responsibility fluorescent sensors,the Förster resonance energy transfer(FRET)technique offers a route to provide clear insights into physiological and pathological processes.In the study,molecular docking,the fluorescent probes of Phi and Swe for FRET were designed and synthesized.FRET analysis shown that Swe and Phi concurrently acted on the PH domain and allosterically inhibited pocket of AKT,respectively.The combination of Swe and Phi significantly increased the heat stability of AKT and decreased protease-induced degeneration.In lipopolysaccharides(LPS)-induced mice and cells,the combination arrested AKT activation,nuclear factor kappa-B(NF-κB)phosphorylation,and the expression of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-8.In conclusion,FRET revealed Phi and Swe concurrently targeted AKT on different domains and the combination of Phi and Swe enhanced the anti-inflammatory effect.
文摘目的了解“互联网+”教学模式在中医护理教学中的应用现状及效果,为提高中医护理信息化教学提供参考。方法系统检索PubMed、Web of Science、Cochrane Library、Embase、中国知网、万方数据知识服务平台、维普数据库、中国生物医学文献数据库中“互联网+”教学模式在中医护理教学中应用的相关文献,检索时限为建库至2024年9月30日,对纳入的文献进行资料汇总与分析。结果共纳入19篇文献,教学方式多采用线上+线下混合教学模式;评价指标多样但无统一标准,除课程成绩外,还包括自主学习能力、批判性思维等指标。结论“互联网+”教学模式虽在中医护理教学中应用广泛,但实践过程中存在教学方式和评价体系有待优化的问题,后续需进一步完善。
文摘以CoO和Pt催化剂(Co-Pt催化剂)作为二元催化剂并将其应用到锂空气电池中,通过场发射扫描电镜和X射线粉末衍射进行物理表征,采用循环伏安、交流阻抗和恒流充放电法研究样品的电化学性能。结果表明,Co-Pt二元催化剂具有良好的电化学性能,在0.025×10–3A·cm^(–2)的电流密度下首次放电比容量和能量密度分别可达到2225.8 m Ah·g^(–1)和5822.8 m Wh·g^(–1)。
