目的对源自泰国红树林底泥中的耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行化学成分和生物活性的研究。方法综合利用硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱(HPLC)等多种方法对耐酸真菌Aspergillus fumigatus OU...目的对源自泰国红树林底泥中的耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行化学成分和生物活性的研究。方法综合利用硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱(HPLC)等多种方法对耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行分离;采用核磁共振谱(NMR)、质谱(MS)、紫外光谱(UV)等现代波谱学技术对分离得到的化合物进行结构鉴定;以人慢性髓性白血病细胞K562及人结肠癌细胞HCT116两株肿瘤细胞为研究模型,采用MTT法和CCK-8法对所分离的化合物进行细胞毒活性评价。结果从耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次级代谢产物中分离得到6个吲哚二酮哌嗪类生物碱:tryprostatins B (1),fumitremorgin C (2),spirotryprostatin A (3),spirotryprostatin.B(4),6-methoxyspirotryprostatin B(5),8,9-dihydroxyspirotrypr-ostatin A (6)以及1个酰胺类化合物cephalimysin B (7)。结论生物活性初步评价结果显示,化合物3和6对人结肠癌细胞HCT116以及人慢性髓性白血病细胞K562具有不同程度的抑制活性。展开更多
Lung cancer is the most common but fatal malignant tumor worldwide.Patients with lung cancer experienced a relatively low 5-year overall survival rate,and issues such as metastasis and drug resistance remain prominent...Lung cancer is the most common but fatal malignant tumor worldwide.Patients with lung cancer experienced a relatively low 5-year overall survival rate,and issues such as metastasis and drug resistance remain prominent challenges in its clinical management.Neddylation,a novel type of post-translational modification,was overactivated in lung cancer and was closely associated with its occurrence,development,metastasis,and drug resistance.This review systematically summarizes the biological process of neddylation and deeply explores the latest research progress on how neddylation affects lung cancer cell proliferation,metastasis,and drug resistance mechanisms,with a focus on its regulation of key molecules such as Cullin-RING E3 ligases and the SCCRO family.Meanwhile,it concludes the current advances in potential therapeutic agents targeting neddylation-related targets,including small-molecule compounds(such as Pevonedistat)and natural extracts(such as arctigenin).Finally,the review prospectively evaluates the application potential and questions requiring further exploration of neddylation in lung cancer treatment.In conclusion,we aim to systematically summarize the biological process of neddylation,critically explore its roles in lung cancer proliferation,metastasis,and drug resistance,and evaluate the therapeutic potential of neddylation-targeting agents.展开更多
目的分析糖化血红蛋白(glycated hemoglobin A1c,HbA1c)、白蛋白/肌酐比值(urinary albumin to creati-nine ratio,UACR)、肾小球滤过率(glomerular filtration rate,GFR)与糖尿病肾病(diabetic nephropathy,DN)患者临床分期的相关性。...目的分析糖化血红蛋白(glycated hemoglobin A1c,HbA1c)、白蛋白/肌酐比值(urinary albumin to creati-nine ratio,UACR)、肾小球滤过率(glomerular filtration rate,GFR)与糖尿病肾病(diabetic nephropathy,DN)患者临床分期的相关性。方法选取2022年1月—2023年12月枝江市人民医院收治的178例DN患者,设为研究组,参考DN临床分期标准,将DN患者分为Ⅰ期组、Ⅱ期组、Ⅲ期组、Ⅳ期组、Ⅴ期组,并选取本院同一时间段的178例糖尿病患者,设为参照组,分析参照组与研究组血糖指标、UACR、GFR水平差异,比较不同严重程度DN患者的HbA1c、肾功能,并分析HbA1c、肾功能指标与DN患者临床分期的相关性。结果参照组与研究组患者空腹血糖、餐后2 h血糖水平比较,差异均无统计学意义(P均>0.05);但研究组的HbA1c水平较参照组高,差异有统计学意义(P<0.05)。与参照组比较,研究组UACR水平更高,GFR水平更低,差异均有统计学意义(P均<0.05)。不同严重程度DN的HbA1c、UACR、GFR水平比较,差异均有统计学意义(P均<0.05)。Pear-son分析结果显示,HbA1c、UACR与DN患者临床分期呈正相关,GFR与DN患者临床分期呈负相关。结论不同临床分期的DN患者血糖及肾功能指标存在显著差异,临床分期更高的DN患者血糖水平更高、肾损伤更加严重。通过检测患者的HbA1c、UACR、GFR水平,有助于临床上诊断DN及评估其病情严重程度。展开更多
文摘目的对源自泰国红树林底泥中的耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行化学成分和生物活性的研究。方法综合利用硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱(HPLC)等多种方法对耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行分离;采用核磁共振谱(NMR)、质谱(MS)、紫外光谱(UV)等现代波谱学技术对分离得到的化合物进行结构鉴定;以人慢性髓性白血病细胞K562及人结肠癌细胞HCT116两株肿瘤细胞为研究模型,采用MTT法和CCK-8法对所分离的化合物进行细胞毒活性评价。结果从耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次级代谢产物中分离得到6个吲哚二酮哌嗪类生物碱:tryprostatins B (1),fumitremorgin C (2),spirotryprostatin A (3),spirotryprostatin.B(4),6-methoxyspirotryprostatin B(5),8,9-dihydroxyspirotrypr-ostatin A (6)以及1个酰胺类化合物cephalimysin B (7)。结论生物活性初步评价结果显示,化合物3和6对人结肠癌细胞HCT116以及人慢性髓性白血病细胞K562具有不同程度的抑制活性。
基金supported by the National Natural Science Foundation of China(No.82574683)the National Natural Science Foundation of Science and Technology Department of Sichuan Province(Nos.2023NSFSC1928 and 2023NSFSC1992)+2 种基金Project of State Administration of Traditional Chinese Medicine of China(No.ZYYCXTD-D-202209)Project of Sichuan Provincial Administration of Traditional Chinese Medicine(No.2022C001)Fundamental Research Funds for the Central Universities(No.YJ201880).
文摘Lung cancer is the most common but fatal malignant tumor worldwide.Patients with lung cancer experienced a relatively low 5-year overall survival rate,and issues such as metastasis and drug resistance remain prominent challenges in its clinical management.Neddylation,a novel type of post-translational modification,was overactivated in lung cancer and was closely associated with its occurrence,development,metastasis,and drug resistance.This review systematically summarizes the biological process of neddylation and deeply explores the latest research progress on how neddylation affects lung cancer cell proliferation,metastasis,and drug resistance mechanisms,with a focus on its regulation of key molecules such as Cullin-RING E3 ligases and the SCCRO family.Meanwhile,it concludes the current advances in potential therapeutic agents targeting neddylation-related targets,including small-molecule compounds(such as Pevonedistat)and natural extracts(such as arctigenin).Finally,the review prospectively evaluates the application potential and questions requiring further exploration of neddylation in lung cancer treatment.In conclusion,we aim to systematically summarize the biological process of neddylation,critically explore its roles in lung cancer proliferation,metastasis,and drug resistance,and evaluate the therapeutic potential of neddylation-targeting agents.
