目的探讨黄芪甲苷Ⅳ对神经毒素多巴胺诱导的帕金森病(PD)模型SH-SY5Y细胞凋亡的影响及相关机制。方法取SH-SY5Y细胞株进行培养至对数生长期分为6组:对照组(正常培养)、模型组(多巴胺诱导)、低剂量组(多巴胺+10 mg/ml黄芪甲苷Ⅳ)、中剂量...目的探讨黄芪甲苷Ⅳ对神经毒素多巴胺诱导的帕金森病(PD)模型SH-SY5Y细胞凋亡的影响及相关机制。方法取SH-SY5Y细胞株进行培养至对数生长期分为6组:对照组(正常培养)、模型组(多巴胺诱导)、低剂量组(多巴胺+10 mg/ml黄芪甲苷Ⅳ)、中剂量组(多巴胺+30 mg/ml黄芪甲苷Ⅳ)、高剂量组(多巴胺+50 mg/ml黄芪甲苷Ⅳ)与酪氨酸蛋白激酶(JAK)抑制组(多巴胺+30 mg/ml黄芪甲苷Ⅳ+30μmol/L AG490),比较各组细胞存活率、细胞凋亡率、活性氧、JAK2、信号转导子和转录激活子3(STAT3)mRNA、磷酸化JAK2(p-JAK2)、磷酸化STAT3(p-STAT3)蛋白表达。结果与对照组比较,模型组、低、中剂量组及JAK抑制组细胞存活率明显降低(P<0.05),其他5组细胞凋亡率和活性氧水平更高,JAK2和STAT3 mRNA、p-JAK2和p-STAT3蛋白表达更低(P<0.05);与模型组比较,中、高剂量组细胞存活率、JAK2和STAT3 mRNA、p-JAK2及p-STAT3蛋白表达更高,低、中、高剂量组细胞凋亡率和活性氧水平更低(P<0.05)。低、中、高剂量组JAK2和STAT3 mRNA、p-JAK2及p-STAT3蛋白表达逐渐升高(0.50±0.03 vs 0.66±0.02 vs 0.81±0.03,0.33±0.02 vs 0.56±0.03 vs 0.77±0.05,0.24±0.03 vs 0.42±0.02 vs 0.55±0.04,0.36±0.03 vs 0.56±0.03 vs 0.73±0.04,P<0.05);JAK抑制组JAK2和STAT3 mRNA、p-JAK2及p-STAT3蛋白表达明显低于模型组(P<0.05)。结论黄芪甲苷Ⅳ能抑制多巴胺诱导的PD模型SH-SY5Y细胞凋亡及氧化应激水平,可能与JAK2/STAT3信号通路上调有关。展开更多
森林火灾范围大距离远,火灾图像中有效特征提取尺寸较大,传统卷积网络难以有效学习,另外火灾中烟雾和雾气较为相似,容易造成错误识别。针对上述问题,提出一种基于多尺度空洞卷积自编码器(Multi-Scale Dilated Convolution Auto Encoder,...森林火灾范围大距离远,火灾图像中有效特征提取尺寸较大,传统卷积网络难以有效学习,另外火灾中烟雾和雾气较为相似,容易造成错误识别。针对上述问题,提出一种基于多尺度空洞卷积自编码器(Multi-Scale Dilated Convolution Auto Encoder,MSDCAE)的深度网络,通过空洞卷积获得不同尺寸的感受野特征并连接输出来优化特征学习,再基于Softmaxwithloss设计改进的损失函数(Improved Softmaxwithloss,ISWL)来提升烟雾、雾气等相似图像的分类性能。实验验证了MSDCAE自编码器和ISWL损失函数的有效性,结果证明在森林火灾的烟火图像识别中,新方法对比普通深度网络算法更具优越性。展开更多
The molecular compositions and com- pound-specific carbon isotope compositions of indi- vidual long-chain n-alkanes of atmospheric aerosols collected during two severe Asian dust episodes in Qingdao in spring of 2002 ...The molecular compositions and com- pound-specific carbon isotope compositions of indi- vidual long-chain n-alkanes of atmospheric aerosols collected during two severe Asian dust episodes in Qingdao in spring of 2002 were analyzed using gas chromatography/mass spectrometry (GC/MS) and gas chromatography/isotope ratio mass spectrometry (GC/IRMS). Typical plant wax n-alkanes (C29 and C31) had lower δ 13C values than those from anthropo- genic (engine exhaust) sources (C21―C23). The av- erage δ 13C value of plant wax n-alkane C29 in non-dust episode periods was -30.5‰ (?30.3‰― ?31.9‰), while -31.3‰ (?31.1‰―?31.5‰) in dust episode periods; for C31, it was ?31.4‰ (?31.1‰― ?33.0‰) in non-dust episode periods, and ?31.7‰ (?31.3‰―?32.6‰) in dust episode periods. Plant wax in the dust episode samples was mainly from herbaceous plants via long-range transport, while local plant wax was mainly from deciduous plants and woody plants. In North China coast, 83.3% of the plant wax in the severe dust episode samples was from C3 plants while 80.0% for the non-dust samples, indicating that plant wax transported to the north- western Pacific Ocean by airborne dust from East Asia was mainly from C3 plants. The results suggestthat the molecular and molecular-isotopic composi- tions of individual long-chain n-alkanes can, as an effective indicator, identify the terrestrial organic components in the dust from East Asia and sedi- ments in the northwest Pacific Ocean.展开更多
文摘目的探讨黄芪甲苷Ⅳ对神经毒素多巴胺诱导的帕金森病(PD)模型SH-SY5Y细胞凋亡的影响及相关机制。方法取SH-SY5Y细胞株进行培养至对数生长期分为6组:对照组(正常培养)、模型组(多巴胺诱导)、低剂量组(多巴胺+10 mg/ml黄芪甲苷Ⅳ)、中剂量组(多巴胺+30 mg/ml黄芪甲苷Ⅳ)、高剂量组(多巴胺+50 mg/ml黄芪甲苷Ⅳ)与酪氨酸蛋白激酶(JAK)抑制组(多巴胺+30 mg/ml黄芪甲苷Ⅳ+30μmol/L AG490),比较各组细胞存活率、细胞凋亡率、活性氧、JAK2、信号转导子和转录激活子3(STAT3)mRNA、磷酸化JAK2(p-JAK2)、磷酸化STAT3(p-STAT3)蛋白表达。结果与对照组比较,模型组、低、中剂量组及JAK抑制组细胞存活率明显降低(P<0.05),其他5组细胞凋亡率和活性氧水平更高,JAK2和STAT3 mRNA、p-JAK2和p-STAT3蛋白表达更低(P<0.05);与模型组比较,中、高剂量组细胞存活率、JAK2和STAT3 mRNA、p-JAK2及p-STAT3蛋白表达更高,低、中、高剂量组细胞凋亡率和活性氧水平更低(P<0.05)。低、中、高剂量组JAK2和STAT3 mRNA、p-JAK2及p-STAT3蛋白表达逐渐升高(0.50±0.03 vs 0.66±0.02 vs 0.81±0.03,0.33±0.02 vs 0.56±0.03 vs 0.77±0.05,0.24±0.03 vs 0.42±0.02 vs 0.55±0.04,0.36±0.03 vs 0.56±0.03 vs 0.73±0.04,P<0.05);JAK抑制组JAK2和STAT3 mRNA、p-JAK2及p-STAT3蛋白表达明显低于模型组(P<0.05)。结论黄芪甲苷Ⅳ能抑制多巴胺诱导的PD模型SH-SY5Y细胞凋亡及氧化应激水平,可能与JAK2/STAT3信号通路上调有关。
基金This work was supported by the National Natural Science Foundation of China （Grant Nos： 40276016, 90211022）.
文摘The molecular compositions and com- pound-specific carbon isotope compositions of indi- vidual long-chain n-alkanes of atmospheric aerosols collected during two severe Asian dust episodes in Qingdao in spring of 2002 were analyzed using gas chromatography/mass spectrometry (GC/MS) and gas chromatography/isotope ratio mass spectrometry (GC/IRMS). Typical plant wax n-alkanes (C29 and C31) had lower δ 13C values than those from anthropo- genic (engine exhaust) sources (C21―C23). The av- erage δ 13C value of plant wax n-alkane C29 in non-dust episode periods was -30.5‰ (?30.3‰― ?31.9‰), while -31.3‰ (?31.1‰―?31.5‰) in dust episode periods; for C31, it was ?31.4‰ (?31.1‰― ?33.0‰) in non-dust episode periods, and ?31.7‰ (?31.3‰―?32.6‰) in dust episode periods. Plant wax in the dust episode samples was mainly from herbaceous plants via long-range transport, while local plant wax was mainly from deciduous plants and woody plants. In North China coast, 83.3% of the plant wax in the severe dust episode samples was from C3 plants while 80.0% for the non-dust samples, indicating that plant wax transported to the north- western Pacific Ocean by airborne dust from East Asia was mainly from C3 plants. The results suggestthat the molecular and molecular-isotopic composi- tions of individual long-chain n-alkanes can, as an effective indicator, identify the terrestrial organic components in the dust from East Asia and sedi- ments in the northwest Pacific Ocean.
