Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interacti...Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interaction dynamics between the cotton plant host and V.dahliae pathogen pose a crucial predicament for effectively managing cotton Verticillium wilt.Nevertheless,the most cost-effective approach to controlling this disease involves breeding and cul-tivating resistant cotton varieties,demanding a meticulous analysis of the mechanisms underlying cotton’s resistance to Verticillium wilt and the identification of pivotal genes.These aspects constitute focal points in disease-resistance breeding programs.In this review,we comprehensively discuss genetic inheritance associated with Verticillium wilt resistance in cotton,the advancements in molecular markers for disease resistance,the functional investiga-tion of resistance genes in cotton,the analysis of pathogenicity genes in V.dahliae,as well as the intricate interplay between cotton and this fungus.Moreover,we delve into the future prospects of cutting-edge research on cotton Verticillium wilt,aiming to proffer valuable insights for the effective management of this devastating fungus.展开更多
以冀棉11和鲁棉研21为供试材料,以枯草芽孢杆菌拌种为药剂对照,并设空白对照,分别于温室和田间进行药效试验,评估种子处理剂"入田"拌种处理对棉花枯黄萎病的防治效果。结果表明,2个拌种剂量均可以显著减轻枯黄萎病发生,拌种...以冀棉11和鲁棉研21为供试材料,以枯草芽孢杆菌拌种为药剂对照,并设空白对照,分别于温室和田间进行药效试验,评估种子处理剂"入田"拌种处理对棉花枯黄萎病的防治效果。结果表明,2个拌种剂量均可以显著减轻枯黄萎病发生,拌种用药制剂量为10 m L·kg^-1(以种子质量计,下同)时对棉花枯萎病防治效果较好,播种后20 d为55.4%,显著高于药剂对照(50.2%);拌种用药制剂量为20 m L·kg^-1时对棉花黄萎病的防治效果最佳,播种后90 d时对黄萎病的防效为47.4%,显著高于药剂对照(31.4%)。推荐"入田"种子处理剂用于防治棉花枯黄萎病的使用剂量为10~20 m L·kg^-1。展开更多
目前,枯草芽孢杆菌已广泛应用于防治棉花黄萎病,但不同菌株的防治效果(防效)参差不齐,有待进一步筛选验证。前期,本课题组从健康棉花植株和根际土壤中分离获得了48株枯草芽孢杆菌。为明确这些菌株对棉花黄萎病的防效,筛选具有优异防效...目前,枯草芽孢杆菌已广泛应用于防治棉花黄萎病,但不同菌株的防治效果(防效)参差不齐,有待进一步筛选验证。前期,本课题组从健康棉花植株和根际土壤中分离获得了48株枯草芽孢杆菌。为明确这些菌株对棉花黄萎病的防效,筛选具有优异防效的枯草芽孢杆菌菌株,采取对峙培养法、对扣培养法测定其对棉花黄萎病菌的抑制作用,并通过温室试验验证其对棉花黄萎病的防效。通过对峙培养和对扣培养筛选出18株拮抗效果较好的菌株。温室试验结果显示:无论浸种或灌根处理,EBS03、EBS07、EBS10和BS04菌株对棉花黄萎病的防效均大于55%,其中EBS03防效最好,浸种与灌根处理对棉花黄萎病的防效分别为76.31%、87.10%;此外,播种后60 d EBS03和EBS10菌株浸种处理的棉苗株高、地上部鲜物质质量、总鲜物质质量较对照分别提高78.08%和77.77%、113.33%和108.00%、107.23%和103.61%。综上,EBS03和EBS10菌株具有较好的促生长作用,并对棉花黄萎病有一定的防效,具有进一步开发应用的潜力。展开更多
基金supported by National Natural Science Foundation of China(32201752)Xinjiang Tianchi Talents Program (TCYC2023TP02)Key Project of the Natural Science Foundation of Xinjiang Production and Construction Corps (2024DA001)
文摘Verticillium wilt,caused by the infamous pathogen Verticillium dahliae,presents a primary constraint on cotton cul-tivation worldwide.The complexity of disease resistance in cotton and the largely unexplored interaction dynamics between the cotton plant host and V.dahliae pathogen pose a crucial predicament for effectively managing cotton Verticillium wilt.Nevertheless,the most cost-effective approach to controlling this disease involves breeding and cul-tivating resistant cotton varieties,demanding a meticulous analysis of the mechanisms underlying cotton’s resistance to Verticillium wilt and the identification of pivotal genes.These aspects constitute focal points in disease-resistance breeding programs.In this review,we comprehensively discuss genetic inheritance associated with Verticillium wilt resistance in cotton,the advancements in molecular markers for disease resistance,the functional investiga-tion of resistance genes in cotton,the analysis of pathogenicity genes in V.dahliae,as well as the intricate interplay between cotton and this fungus.Moreover,we delve into the future prospects of cutting-edge research on cotton Verticillium wilt,aiming to proffer valuable insights for the effective management of this devastating fungus.
文摘以冀棉11和鲁棉研21为供试材料,以枯草芽孢杆菌拌种为药剂对照,并设空白对照,分别于温室和田间进行药效试验,评估种子处理剂"入田"拌种处理对棉花枯黄萎病的防治效果。结果表明,2个拌种剂量均可以显著减轻枯黄萎病发生,拌种用药制剂量为10 m L·kg^-1(以种子质量计,下同)时对棉花枯萎病防治效果较好,播种后20 d为55.4%,显著高于药剂对照(50.2%);拌种用药制剂量为20 m L·kg^-1时对棉花黄萎病的防治效果最佳,播种后90 d时对黄萎病的防效为47.4%,显著高于药剂对照(31.4%)。推荐"入田"种子处理剂用于防治棉花枯黄萎病的使用剂量为10~20 m L·kg^-1。
文摘目前,枯草芽孢杆菌已广泛应用于防治棉花黄萎病,但不同菌株的防治效果(防效)参差不齐,有待进一步筛选验证。前期,本课题组从健康棉花植株和根际土壤中分离获得了48株枯草芽孢杆菌。为明确这些菌株对棉花黄萎病的防效,筛选具有优异防效的枯草芽孢杆菌菌株,采取对峙培养法、对扣培养法测定其对棉花黄萎病菌的抑制作用,并通过温室试验验证其对棉花黄萎病的防效。通过对峙培养和对扣培养筛选出18株拮抗效果较好的菌株。温室试验结果显示:无论浸种或灌根处理,EBS03、EBS07、EBS10和BS04菌株对棉花黄萎病的防效均大于55%,其中EBS03防效最好,浸种与灌根处理对棉花黄萎病的防效分别为76.31%、87.10%;此外,播种后60 d EBS03和EBS10菌株浸种处理的棉苗株高、地上部鲜物质质量、总鲜物质质量较对照分别提高78.08%和77.77%、113.33%和108.00%、107.23%和103.61%。综上,EBS03和EBS10菌株具有较好的促生长作用,并对棉花黄萎病有一定的防效,具有进一步开发应用的潜力。
