目的:基于网络药理学研究半夏泻心汤治疗慢性萎缩性胃炎的作用机制。方法:利用TCMSP数据库,得到半夏泻心汤成分的口服利用率和药物相似性等参数,并筛选药物的有效成分组,收集有效成分的靶点。通过Dis Ge NET数据库得到慢性萎缩性胃炎的...目的:基于网络药理学研究半夏泻心汤治疗慢性萎缩性胃炎的作用机制。方法:利用TCMSP数据库,得到半夏泻心汤成分的口服利用率和药物相似性等参数,并筛选药物的有效成分组,收集有效成分的靶点。通过Dis Ge NET数据库得到慢性萎缩性胃炎的疾病靶点,在与半夏泻心汤靶点取得交集后得到了药效靶点。将上述靶点输入到String数据库进行蛋白质-蛋白质相互作用网络预测,最后利用DAVID数据库进行KEGG富集分析,阐明半夏泻心汤治疗慢性萎缩性胃炎的潜在信号通路。结果:我们筛选出了半夏泻心汤194个活性成分和154个作用靶点。通过蛋白质互作网络分析,半夏泻心汤治疗慢性萎缩性胃炎可能与BCL-2,PTGS2,TNF,GSTP1,GSTM1等靶点有关。KEGG富集分析结果显示半夏泻心汤治疗慢性萎缩性胃炎与TNF信号通路,NF-κB信号通路,NOD样受体信号通路,PI3k/Akt信号通路,Toll样受体信号通路等有关。结论:半夏泻心汤治疗慢性萎缩性胃炎具有中药复方多成分-多靶点-多通路的特点,其治疗慢性萎缩性胃炎的生物学机制可能与TNF signaling pathway等信号通路有关。展开更多
OBJECTIVE:To investigate the targets and mechanisms of action of Qingkailing injection(清开灵注射液,QKL)in the treatment of cholestatic hepatitis.METHODS:A network pharmacology method was implemented using drug and di...OBJECTIVE:To investigate the targets and mechanisms of action of Qingkailing injection(清开灵注射液,QKL)in the treatment of cholestatic hepatitis.METHODS:A network pharmacology method was implemented using drug and disease databases to target QKL and cholestasis hepatitis,respectively.The functional protein association network STRING database was used to construct a protein-protein interaction network using R language and the Bioconductor toolkit.The org.Hs.eg.db and cluster Profiler packages were used for gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis,which explored biological functions and pathways of potential targets.Targets were then visualized using Cytoscape 3.6.0 software.RESULTS:We screened 121 compounds in QKL and identified 112 targets for the treatment of cholestatic hepatitis.QKL played a role in the treatment of cholestatic hepatitis through 305 biology process terms,15 cellular component and 29 molecular function terms.The mechanism of QKL action was mainly related to tumor necrosis factor,mitogen-activated protein kinase,and PI3 K-Akt signaling pathways.CONCLUSION:The treatment of cholestatic hepatitis by QKL involved multiple targets,biological functions,and signaling pathways that are closely associated with the disease.展开更多
文摘目的:基于网络药理学研究半夏泻心汤治疗慢性萎缩性胃炎的作用机制。方法:利用TCMSP数据库,得到半夏泻心汤成分的口服利用率和药物相似性等参数,并筛选药物的有效成分组,收集有效成分的靶点。通过Dis Ge NET数据库得到慢性萎缩性胃炎的疾病靶点,在与半夏泻心汤靶点取得交集后得到了药效靶点。将上述靶点输入到String数据库进行蛋白质-蛋白质相互作用网络预测,最后利用DAVID数据库进行KEGG富集分析,阐明半夏泻心汤治疗慢性萎缩性胃炎的潜在信号通路。结果:我们筛选出了半夏泻心汤194个活性成分和154个作用靶点。通过蛋白质互作网络分析,半夏泻心汤治疗慢性萎缩性胃炎可能与BCL-2,PTGS2,TNF,GSTP1,GSTM1等靶点有关。KEGG富集分析结果显示半夏泻心汤治疗慢性萎缩性胃炎与TNF信号通路,NF-κB信号通路,NOD样受体信号通路,PI3k/Akt信号通路,Toll样受体信号通路等有关。结论:半夏泻心汤治疗慢性萎缩性胃炎具有中药复方多成分-多靶点-多通路的特点,其治疗慢性萎缩性胃炎的生物学机制可能与TNF signaling pathway等信号通路有关。
基金Supported by a grant from the National Natural Science Foundation of China(Study on Qingkailing’s Intervention Mechanism on"No Reflow"Phenomenon after Cerebral Infarction and Pericyte"Rho A/ROCK"Pathway,No.81973789)。
文摘OBJECTIVE:To investigate the targets and mechanisms of action of Qingkailing injection(清开灵注射液,QKL)in the treatment of cholestatic hepatitis.METHODS:A network pharmacology method was implemented using drug and disease databases to target QKL and cholestasis hepatitis,respectively.The functional protein association network STRING database was used to construct a protein-protein interaction network using R language and the Bioconductor toolkit.The org.Hs.eg.db and cluster Profiler packages were used for gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis,which explored biological functions and pathways of potential targets.Targets were then visualized using Cytoscape 3.6.0 software.RESULTS:We screened 121 compounds in QKL and identified 112 targets for the treatment of cholestatic hepatitis.QKL played a role in the treatment of cholestatic hepatitis through 305 biology process terms,15 cellular component and 29 molecular function terms.The mechanism of QKL action was mainly related to tumor necrosis factor,mitogen-activated protein kinase,and PI3 K-Akt signaling pathways.CONCLUSION:The treatment of cholestatic hepatitis by QKL involved multiple targets,biological functions,and signaling pathways that are closely associated with the disease.
