OBJECTIVE:To examine the influence of SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae)(SSPHⅠ)on hepatocellular carcinoma(HCC)metastasis,and to elucidate the underlying mechanism.METHODS:The intrahepatic m...OBJECTIVE:To examine the influence of SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae)(SSPHⅠ)on hepatocellular carcinoma(HCC)metastasis,and to elucidate the underlying mechanism.METHODS:The intrahepatic metastasis Bagg's Albino/c(BALB/c)mouse model was established with human hepatocellular carcinomas(HepG2)cells,then treated with normal saline(once per day),cisplatin(2 mg/kg,once every 2 d),and SSPHⅠ(25,50,and 75 mg/kg,once per day).Then,we assessed alterations in the hepatic pathology and target protein expressions in the intrahepatic metastasis BALB/c mouse model using a series of molecular biology techniques.RESULTS:Based on our analysis,SSPHⅠsignificantly alleviated hepatocyte necrosis and tumor cells infiltration.Moreover,SSPHⅠsuppressed extracellular matrix(ECM)degradation and angiogenesis via a decrease in matrix etalloproteinase-2(MMP-2),MMP-9,CD31,CD34,and vascular endothelial growth factor(VEGF)levels.Furthermore,SSPHⅠrepressed invasion and metastasis by suppressing the transforming growth factor-β1(TGF-β1)/Smad7 axis and epithelial-mesenchymal transition(EMT),as evidenced by the scarce TGF-β1,Ncadherin,and Vimentin expressions,and elevated Smad7 and E-cadherin expressions.CONCLUSION:The SSPHⅠ-mediated negative regulation of the TGF-β1/Smad7 axis and EMT are critical for the inhibition of HCC invasion and metastasis.展开更多
目的初步调查肺移植(lung transplant,LT)受者术后健康相关生活质量(health-related quality of life,HRQOL)的纵向变化特点,并分析影响因素。方法2022年10月至2023年12月,采用便利抽样法选取广州市某三级甲等医院收治的62例LT受者为研...目的初步调查肺移植(lung transplant,LT)受者术后健康相关生活质量(health-related quality of life,HRQOL)的纵向变化特点,并分析影响因素。方法2022年10月至2023年12月,采用便利抽样法选取广州市某三级甲等医院收治的62例LT受者为研究对象,分别于出院前1 d、术后3个月及6个月3个时间点采用简明健康调查量表(medical outcomes study 36-item short form health survey,SF-36)、广泛性焦虑障碍量表(generalized anxiety disorder-7,GAD-7)、患者健康问卷(patient health questionnaire-9,PHQ-9)、移植症状发生和症状困扰量表(modified transplant symptom occurrence and symptom distress scale,MTSOSD)对其开展纵向调查。结果62例LT受者3个时间点的SF-36总分中位数分别为37.39(24.48,50.29)、59.43(45.48,74.77)、66.86(44.38,83.66)分,随时间推移逐渐升高(P<0.05)。线性混合模型显示,年龄增长、付费方式、术后住院时间延长、抑郁情绪越重是LT受者HRQOL的危险因素(均P<0.05),原发疾病诊断、轻度经济负担是其HRQOL的保护因素(均P<0.05)。结论LT受者术后HRQOL随时间推移逐渐改善,医护人员应针对其年龄、经济负担程度、付费方式、抑郁情绪等因素,制订个体化干预措施,进一步改善其HRQOL。展开更多
基金National Natural Science Foundation of China,a New Anti-cancer Plant drug,SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae),against Invasion and Metastasis of Non-small Cell Lung Cancer and Reversing Tyrosine Kinase Inhibitors Resistance basing on Human Growth Factor/c-Mesenchymal to Epithelial Transition Factor Pathway and its Molecular Mechanism of Regulating Epithelial-Mesenchymal Transition(No.8164062)the Natural Science Foundation of Guangxi Province,Study on the Antihepatic Fibrosis Mechanism of Saponins from Shuitianqi(Rhizoma Schizocapasae Plantagineae)based on Transforming Growth Factor-β/Smad Signaling Pathway(No.2019GXNSFAA245075)。
文摘OBJECTIVE:To examine the influence of SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae)(SSPHⅠ)on hepatocellular carcinoma(HCC)metastasis,and to elucidate the underlying mechanism.METHODS:The intrahepatic metastasis Bagg's Albino/c(BALB/c)mouse model was established with human hepatocellular carcinomas(HepG2)cells,then treated with normal saline(once per day),cisplatin(2 mg/kg,once every 2 d),and SSPHⅠ(25,50,and 75 mg/kg,once per day).Then,we assessed alterations in the hepatic pathology and target protein expressions in the intrahepatic metastasis BALB/c mouse model using a series of molecular biology techniques.RESULTS:Based on our analysis,SSPHⅠsignificantly alleviated hepatocyte necrosis and tumor cells infiltration.Moreover,SSPHⅠsuppressed extracellular matrix(ECM)degradation and angiogenesis via a decrease in matrix etalloproteinase-2(MMP-2),MMP-9,CD31,CD34,and vascular endothelial growth factor(VEGF)levels.Furthermore,SSPHⅠrepressed invasion and metastasis by suppressing the transforming growth factor-β1(TGF-β1)/Smad7 axis and epithelial-mesenchymal transition(EMT),as evidenced by the scarce TGF-β1,Ncadherin,and Vimentin expressions,and elevated Smad7 and E-cadherin expressions.CONCLUSION:The SSPHⅠ-mediated negative regulation of the TGF-β1/Smad7 axis and EMT are critical for the inhibition of HCC invasion and metastasis.
文摘目的初步调查肺移植(lung transplant,LT)受者术后健康相关生活质量(health-related quality of life,HRQOL)的纵向变化特点,并分析影响因素。方法2022年10月至2023年12月,采用便利抽样法选取广州市某三级甲等医院收治的62例LT受者为研究对象,分别于出院前1 d、术后3个月及6个月3个时间点采用简明健康调查量表(medical outcomes study 36-item short form health survey,SF-36)、广泛性焦虑障碍量表(generalized anxiety disorder-7,GAD-7)、患者健康问卷(patient health questionnaire-9,PHQ-9)、移植症状发生和症状困扰量表(modified transplant symptom occurrence and symptom distress scale,MTSOSD)对其开展纵向调查。结果62例LT受者3个时间点的SF-36总分中位数分别为37.39(24.48,50.29)、59.43(45.48,74.77)、66.86(44.38,83.66)分,随时间推移逐渐升高(P<0.05)。线性混合模型显示,年龄增长、付费方式、术后住院时间延长、抑郁情绪越重是LT受者HRQOL的危险因素(均P<0.05),原发疾病诊断、轻度经济负担是其HRQOL的保护因素(均P<0.05)。结论LT受者术后HRQOL随时间推移逐渐改善,医护人员应针对其年龄、经济负担程度、付费方式、抑郁情绪等因素,制订个体化干预措施,进一步改善其HRQOL。
