Integral to the urban ecosystem,greening trees provide many ecological benefits,but the active biogenic volatile organic compounds(BVOCs)they release contribute to the production of ozone and secondary organic aerosol...Integral to the urban ecosystem,greening trees provide many ecological benefits,but the active biogenic volatile organic compounds(BVOCs)they release contribute to the production of ozone and secondary organic aerosols,which harm ambient air quality.It is,therefore,necessary to understand the BVOC emission characteristics of dominant greening tree species and their relative contribution to secondary pollutants in various urban contexts.Consequently,this study utilized a dynamic enclosure system to collect BVOC samples of seven dominant greening tree species in urban Chengdu,Southwest China.Gas chromatography/mass spectrometry was used to analyze the BVOC components and standardized BVOC emission rates of each tree species were then calculated to assess their relative potential to form secondary pollutants.We found obvious differences in the composition of BVOCs emitted by each species.Ficus virens displayed a high isoprene emission rate at31.472μgC/(gdw(g dry weight)·hr),while Cinnamomum camphora emitted high volumes of D-Limonene at 93.574μgC/(gdw·hr).In terms of the BVOC emission rates by leaf area,C.camphora had the highest emission rate of total BVOCs at 13,782.59μgC/(m^(2)·hr),followed by Cedrus deodara with 5466.86μgC/(m^(2)·hr).Ginkgo biloba and Osmanthus fragrans mainly emitted oxygenated VOCs with lower overall emission rates.The high BVOC emitters like F.virens,C.camphora,and Magnolia grandiflora have high potential for significantly contributing to environmental secondary pollutants,so should be cautiously considered for future planting.This study provides important implications for improving urban greening efforts for subtropical Chinese urban contexts,like Chengdu.展开更多
OBJECTIVE: To evaluate the indicators of an animal model of gouty arthritis(GA) with dampness heat accumulation and the intervention effect of Tongfeng Qingxiao formula(痛风清消方, TFQXF). METHODS: Seventy-two healthy...OBJECTIVE: To evaluate the indicators of an animal model of gouty arthritis(GA) with dampness heat accumulation and the intervention effect of Tongfeng Qingxiao formula(痛风清消方, TFQXF). METHODS: Seventy-two healthy adult Sprague-Dawley male rats were selected and randomly divided into a normal group, model group, low-dose group, mediumdose group, high-dose group, and diclofenac group using a random number table method, with 12 rats in each group. After group intervention, the general condition of the rats in each group was monitored and recorded, and the swelling index was measured. After separating the serum, the changes in glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase(AST), carbamide(UREA), creatinine(CREA), triglyceride(TG), total serum cholesterol(TC), low density lipoprotein cholesterol(LDL-C), and high density lipoprotein cholesterol(HDL-C) in the serum of the rats in each group were measured using an automatic biochemical analyzer. The levels of motilin(MTL), gastrin(GAS), endothelin(ET), calcitonin gene-related peptide(CGRP), heat shock protein 70(HSP70), interleukin(IL)-1β, and nuclear factor kappa-B(NF-κB) in the serum of the rats in each group were evaluated using enzyme-linked immunosorbent assay(ELISA) kits. Kidney tissues were used to evaluate the protein and m RNA expression of aquaporin(AQP) 1 and AQP2. Colon tissue was used to evaluate the protein and m RNA expression of AQP3 and AQP4 by Western blotting(WB) assay and real-time quantitative polymerase chain reaction(RT-q PCR). The levels of ALT, AST, UREA, and CREA were used to evaluate the liver and kidney function of rats. The levels of MTL and GAS were used to evaluate the gastrointestinal function of rats. The levels of TG, TC, LDL-C, HDL-C, AQP1, AQP2, AQP3, and AQP4 were used to evaluate the "dampness" syndrome performance in rats. The levels of ET, CGRP, and HSP70 were used to evaluate the "heat" syndrome performance in rats. The levels of IL-1β and NF-κB were used to evaluate the degree of inflammation in rats. The pathological changes in synovial and colonic tissues were observed by hematoxylin and eosin staining. RESULTS: Except for the normal group, after modeling treatment, the ankle joint of rats in both the model group and drug treatment groups gradually swelled, reaching a peak at 12 h, and then gradually began to subside. The results of biochemical analyzer detection indicated that the serum ALT, AST, UREA, CREA, TG, TC and LDL-C levels were significantly higher, but the HDL-C level was significantly lower in the rats of the model group than in the rats of the normal group(P < 0.05). The serum ALT, AST, UREA, CREA, MTL, TC and LDL-C levels were significantly lower, but the HDL-C level was significantly higher in the rats of all drug treatment groups than in the rats of the model group(P < 0.05). The results of ELISA detection indicated that the MTL, GAS, ET, HSP70, IL-1β,and NF-κB levels were significantly higher, but the CGRP level was significantly lower in the rats of the model group than in the rats of the normal group(P < 0.05). The levels of MTL, GAS, ET, HSP70, IL-1β, and NF-κB were significantly lower, but CGRP was significantly higher in the rats of the drug treatment groups than in the rats of the model group(P < 0.05). The results of WB and RTq PCR indicated that compared to the normal group, the levels of AQP1 and AQP2 in the model group were significantly higher in the kidney tissue, whereas the levels of AQP3 and AQP4 were significantly lower in the colon tissue(P < 0.05). Compared to those in the model group, the levels of AQP1 and AQP2 in the drug treatment groups were significantly lower in the kidney tissue, whereas the levels of AQP3 and AQP4 were significantly higher in the colon tissue(P < 0.05). In the model group, erosion of the colonic mucosal surface and inflammatory exudate occurred. Some mucosal epithelium had fallen off, the number of glands in the lamina propria was lower, many inflammatory cells infiltrated the interstitial layer, the connective tissue in the submucosa became loose and edematous, and lymph follicles developed. We found a significant proliferation of synovial cells in the ankle joint, an increase in cell density and neovascularization, and visible infiltration of inflammatory cells. The cartilage surface was not smooth. However, each drug group could improve the pathological changes in intestinal and synovial tissues to varying degrees. CONCLUSIONS: Blood lipid metabolism indexes and AQPs could be used as objective evaluation indexes for the "dampness" syndrome performance of damp-heat accumulation type GA. ET, HSP70 and CGRP could be used as objective evaluation indexes for the "heat" syndrome performance, and the immune inflammation index could be used as objective evaluation indexes for the inflammation degree. The overall efficacy of TFQXF in the treatment of damp-heat accumulation-type GA could be determined by adjusting the above objective evaluation indexes. It provided some ideas and directions for clinical risk assessments and drug development of GA.展开更多
For black shales,laminae and bedding are hard to identify,grain size is difficult to measure,and trace fossils do not exist.Taking the Ordovician Wufeng–Silurian Longmaxi shale in southern Sichuan Basin,China,as an e...For black shales,laminae and bedding are hard to identify,grain size is difficult to measure,and trace fossils do not exist.Taking the Ordovician Wufeng–Silurian Longmaxi shale in southern Sichuan Basin,China,as an example,the types,characteristics and models of microfacies in epicontinental shale are analyzed by means of full-scale observation of large thin sections,argon-ion polishing field emission-scanning electron microscopy(FE-SEM),and kerogen microscopy.The epicontinental sea develops delta,tidal flat and shelf facies,with black shale found in microfacies such as the underwater distributary channel and interdistributary bay under delta front facies,the calcareous and clayey flats under intertidal flat facies,the calcareous and clayey shelfs under shallow shelf facies,the deep slope,deep plain and deep depression under deep shelf facies,and the overflow under gravity flow facies.Basinward,silty lamina decreases and clayey lamina increases,the grain size changes from coarse silt to fine mud,the silica content increases from about 20%to above 55%,the carbonate and clay minerals content decreases from above 40%to around 10%,and the kerogen type changes from type II2 to type II1 and type I.Provenance and topography dominate the types and distribution of shale microfacies.The underwater distributary channel,interdistributary bay,clayey flat,clayey shelf,and overflow microfacies are developed in areas with sufficient sediment supply.The calcareous flat and calcareous shelf are developed in areas with insufficient sediment supply.The deep shelf shale area is divided into deep slope,deep plain,and deep depression microfacies as a result of three breaks.The formation of epicontinental shale with different microfacies is closely related to the tectonic setting,paleoclimate,and sea level rise.The relatively active tectonic setting increases the supply of terrigenous clasts,forming muddy water fine-grained sediment.The warm and humid paleoclimate is conducive to the enrichment of organic matter.The rapid sea level rise is helpful to the widespread black shale.展开更多
基金supported by the National Natural Science Foundation of China(No.21906108)the Fundamental Research Funds for the Central Universities(No.YJ201937)+1 种基金Chengdu Science and Technology Bureau(No.2020-YF09-00051-SN)the Sichuan"1000 Plan"Scholar Program
文摘Integral to the urban ecosystem,greening trees provide many ecological benefits,but the active biogenic volatile organic compounds(BVOCs)they release contribute to the production of ozone and secondary organic aerosols,which harm ambient air quality.It is,therefore,necessary to understand the BVOC emission characteristics of dominant greening tree species and their relative contribution to secondary pollutants in various urban contexts.Consequently,this study utilized a dynamic enclosure system to collect BVOC samples of seven dominant greening tree species in urban Chengdu,Southwest China.Gas chromatography/mass spectrometry was used to analyze the BVOC components and standardized BVOC emission rates of each tree species were then calculated to assess their relative potential to form secondary pollutants.We found obvious differences in the composition of BVOCs emitted by each species.Ficus virens displayed a high isoprene emission rate at31.472μgC/(gdw(g dry weight)·hr),while Cinnamomum camphora emitted high volumes of D-Limonene at 93.574μgC/(gdw·hr).In terms of the BVOC emission rates by leaf area,C.camphora had the highest emission rate of total BVOCs at 13,782.59μgC/(m^(2)·hr),followed by Cedrus deodara with 5466.86μgC/(m^(2)·hr).Ginkgo biloba and Osmanthus fragrans mainly emitted oxygenated VOCs with lower overall emission rates.The high BVOC emitters like F.virens,C.camphora,and Magnolia grandiflora have high potential for significantly contributing to environmental secondary pollutants,so should be cautiously considered for future planting.This study provides important implications for improving urban greening efforts for subtropical Chinese urban contexts,like Chengdu.
基金the National Natural Science Foundation of China:a Study on the Use of Tongfengqingxiao for Treating Gouty Arthritis and Neutrophil Extracellular Traps Formation based on the Transforming Growth Factor-β1 Signaling Pathway (No. 81860857)a Study on the Mechanism by which Tongfeng Qingxiao Regulates the Toll-like receptors-myeloid Differentiation Factor 88-nuclear Factor Kappa-B Signaling Pathway in Gouty Arthritis Rats with Gut Dysbiosis and Dampness-Heat Syndrome based on the Gut-enteric-microbiota Axis (No. 82060871)National Natural Science Foundation of Jiangxi Province:a Study on the Regulatory Mechanism of the Toll-like receptors-Myeloid differentiation factor 88-nuclear factor kappa-B Signaling Pathway Related to Dysbacteriosis of the Intestinal Flora with Gouty Arthritis in Rats Mediated by Tongfengqingxiao,based on the “Gut-Microbiota” Axis (No. 20202BAB206071)。
文摘OBJECTIVE: To evaluate the indicators of an animal model of gouty arthritis(GA) with dampness heat accumulation and the intervention effect of Tongfeng Qingxiao formula(痛风清消方, TFQXF). METHODS: Seventy-two healthy adult Sprague-Dawley male rats were selected and randomly divided into a normal group, model group, low-dose group, mediumdose group, high-dose group, and diclofenac group using a random number table method, with 12 rats in each group. After group intervention, the general condition of the rats in each group was monitored and recorded, and the swelling index was measured. After separating the serum, the changes in glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase(AST), carbamide(UREA), creatinine(CREA), triglyceride(TG), total serum cholesterol(TC), low density lipoprotein cholesterol(LDL-C), and high density lipoprotein cholesterol(HDL-C) in the serum of the rats in each group were measured using an automatic biochemical analyzer. The levels of motilin(MTL), gastrin(GAS), endothelin(ET), calcitonin gene-related peptide(CGRP), heat shock protein 70(HSP70), interleukin(IL)-1β, and nuclear factor kappa-B(NF-κB) in the serum of the rats in each group were evaluated using enzyme-linked immunosorbent assay(ELISA) kits. Kidney tissues were used to evaluate the protein and m RNA expression of aquaporin(AQP) 1 and AQP2. Colon tissue was used to evaluate the protein and m RNA expression of AQP3 and AQP4 by Western blotting(WB) assay and real-time quantitative polymerase chain reaction(RT-q PCR). The levels of ALT, AST, UREA, and CREA were used to evaluate the liver and kidney function of rats. The levels of MTL and GAS were used to evaluate the gastrointestinal function of rats. The levels of TG, TC, LDL-C, HDL-C, AQP1, AQP2, AQP3, and AQP4 were used to evaluate the "dampness" syndrome performance in rats. The levels of ET, CGRP, and HSP70 were used to evaluate the "heat" syndrome performance in rats. The levels of IL-1β and NF-κB were used to evaluate the degree of inflammation in rats. The pathological changes in synovial and colonic tissues were observed by hematoxylin and eosin staining. RESULTS: Except for the normal group, after modeling treatment, the ankle joint of rats in both the model group and drug treatment groups gradually swelled, reaching a peak at 12 h, and then gradually began to subside. The results of biochemical analyzer detection indicated that the serum ALT, AST, UREA, CREA, TG, TC and LDL-C levels were significantly higher, but the HDL-C level was significantly lower in the rats of the model group than in the rats of the normal group(P < 0.05). The serum ALT, AST, UREA, CREA, MTL, TC and LDL-C levels were significantly lower, but the HDL-C level was significantly higher in the rats of all drug treatment groups than in the rats of the model group(P < 0.05). The results of ELISA detection indicated that the MTL, GAS, ET, HSP70, IL-1β,and NF-κB levels were significantly higher, but the CGRP level was significantly lower in the rats of the model group than in the rats of the normal group(P < 0.05). The levels of MTL, GAS, ET, HSP70, IL-1β, and NF-κB were significantly lower, but CGRP was significantly higher in the rats of the drug treatment groups than in the rats of the model group(P < 0.05). The results of WB and RTq PCR indicated that compared to the normal group, the levels of AQP1 and AQP2 in the model group were significantly higher in the kidney tissue, whereas the levels of AQP3 and AQP4 were significantly lower in the colon tissue(P < 0.05). Compared to those in the model group, the levels of AQP1 and AQP2 in the drug treatment groups were significantly lower in the kidney tissue, whereas the levels of AQP3 and AQP4 were significantly higher in the colon tissue(P < 0.05). In the model group, erosion of the colonic mucosal surface and inflammatory exudate occurred. Some mucosal epithelium had fallen off, the number of glands in the lamina propria was lower, many inflammatory cells infiltrated the interstitial layer, the connective tissue in the submucosa became loose and edematous, and lymph follicles developed. We found a significant proliferation of synovial cells in the ankle joint, an increase in cell density and neovascularization, and visible infiltration of inflammatory cells. The cartilage surface was not smooth. However, each drug group could improve the pathological changes in intestinal and synovial tissues to varying degrees. CONCLUSIONS: Blood lipid metabolism indexes and AQPs could be used as objective evaluation indexes for the "dampness" syndrome performance of damp-heat accumulation type GA. ET, HSP70 and CGRP could be used as objective evaluation indexes for the "heat" syndrome performance, and the immune inflammation index could be used as objective evaluation indexes for the inflammation degree. The overall efficacy of TFQXF in the treatment of damp-heat accumulation-type GA could be determined by adjusting the above objective evaluation indexes. It provided some ideas and directions for clinical risk assessments and drug development of GA.
基金Supported by the"14th Five-Year Plan"Major Special Project of the Science and Technology Management Department of RIPED(2021DJ1901).
文摘For black shales,laminae and bedding are hard to identify,grain size is difficult to measure,and trace fossils do not exist.Taking the Ordovician Wufeng–Silurian Longmaxi shale in southern Sichuan Basin,China,as an example,the types,characteristics and models of microfacies in epicontinental shale are analyzed by means of full-scale observation of large thin sections,argon-ion polishing field emission-scanning electron microscopy(FE-SEM),and kerogen microscopy.The epicontinental sea develops delta,tidal flat and shelf facies,with black shale found in microfacies such as the underwater distributary channel and interdistributary bay under delta front facies,the calcareous and clayey flats under intertidal flat facies,the calcareous and clayey shelfs under shallow shelf facies,the deep slope,deep plain and deep depression under deep shelf facies,and the overflow under gravity flow facies.Basinward,silty lamina decreases and clayey lamina increases,the grain size changes from coarse silt to fine mud,the silica content increases from about 20%to above 55%,the carbonate and clay minerals content decreases from above 40%to around 10%,and the kerogen type changes from type II2 to type II1 and type I.Provenance and topography dominate the types and distribution of shale microfacies.The underwater distributary channel,interdistributary bay,clayey flat,clayey shelf,and overflow microfacies are developed in areas with sufficient sediment supply.The calcareous flat and calcareous shelf are developed in areas with insufficient sediment supply.The deep shelf shale area is divided into deep slope,deep plain,and deep depression microfacies as a result of three breaks.The formation of epicontinental shale with different microfacies is closely related to the tectonic setting,paleoclimate,and sea level rise.The relatively active tectonic setting increases the supply of terrigenous clasts,forming muddy water fine-grained sediment.The warm and humid paleoclimate is conducive to the enrichment of organic matter.The rapid sea level rise is helpful to the widespread black shale.
