Microorganisms are fundamental for the functioning of marine ecosystems and are involved in the decomposition of organic matter, transformation of nutrients and circulation of biologically-important chemicals. Based o...Microorganisms are fundamental for the functioning of marine ecosystems and are involved in the decomposition of organic matter, transformation of nutrients and circulation of biologically-important chemicals. Based on the complexity of the natural geographic characteristics of the Changjiang River Estuary, the geographic distribution of sedimentary microorganisms and the causes of this distribution are largely unexplored. In this work, the surface sediment samples from the adjacent sea area of the Changjiang River Estuary were collected. Their prokaryotic diversity was examined by high-throughput sequencing technology, and the environmental factors of the bacterial community were investigated. The results indicated that the distribution of prokaryotic communities in the sediments of the study areas showed obvious spatial heterogeneity. The sampling sequences divided the sample regions into three distinct clusters. Each geographic region had a unique community structure, although Proteobacteria, Bacteroidota, Desulfobacterota, Acidobacteriota, and Actinobacteriota all existed in these three branches. Canonical correspondence analysis demonstrated that prokaryotic diversity and community distribution were significantly correlated with the geographic location of sediment, seawater depth, and in particular, nutrient content(e.g., total phosphorus, total organic carbon and dissolved oxygen). Moreover, it was found for the first time that the metal ions obviously affected the composition and distribution of the prokaryotic community in this area. In general, this work provides new insights into the structural characteristics and driving factors of prokaryotic communities under the background of the ever-changing Changjiang River Estuary.展开更多
The 6-4 photolyase can specifically repair 6-pyrimidine-4-pyrimidinone(6-4 PP)photoproduct from UVBtriggered DNA damage.However,its application for skin damage hasn’t been reported yet.This study prepared the novel 6...The 6-4 photolyase can specifically repair 6-pyrimidine-4-pyrimidinone(6-4 PP)photoproduct from UVBtriggered DNA damage.However,its application for skin damage hasn’t been reported yet.This study prepared the novel 6-4 photolyase nanoliposomes(6-4 NL),derived from UVB-resistant Antarctic Chlamydomonas sp.ICE-L,which effectively removed 6-4 PPs in UVB-induced skin.The 6-4 NL mitigated oxidative stress by remarkably reducing ROS,MDA and 8-OHdG,and enhancing SOD activity.Anti-inflammatory action was associated with the inhibition of NF-κB pathways by downregulating TNF-α,IL-6,COX-2 and NF-κB.The 6-4 NL regulated mitogen-activated protein kinase pathways via significantly diminishing expression of JNK,ERK and p38,and their phosphorylated forms.Additionally,anti-wrinkle effects were evidenced by significantly reducing the expression of AP-1,MMP-1 and MMP-2,and increasing hydroxyproline levels.These findings provided convincing evidence for the protective mechanisms of 6-4 NL against UVB-induced skin damage,as well as for the potential application in preventing photoaging and skin diseases.展开更多
Natural oyster zinc-rich peptide(OZP)was extracted and purified.The higher zinc concentration in natural OZP may be closely related to the high zinc affinity of amino acids containing Lys,His,Cys,Val,Arg and Ser resid...Natural oyster zinc-rich peptide(OZP)was extracted and purified.The higher zinc concentration in natural OZP may be closely related to the high zinc affinity of amino acids containing Lys,His,Cys,Val,Arg and Ser residues.FTIR analysis indicated that amino,amide,carboxyl and carbonyl groups may contribute to the binding sites for zinc and peptides.Administration of OZP ameliorated the symptoms of DSS-induced ulcerative colitis(UC)in mice,including weight loss,colonic damage,mucosal edema,goblet cell exhaustion and crypt disappearance.Natural OZP suppressed oxidative stress by significantly reducing the level of NO,MDA and MPO as well as increasing SOD activity.The anti-inflammatory effects of OZP were associated with the inhibition of TLR4/NF-κB pathways by diminishing NF-κB,TLR4,COX-2,iNOS,TNF-α,IL-6 and IFN-γand elevating IL-10.By promoting mucus secretion,repairing goblet cells and enhancing the expression of tight junction proteins including occludin,claudin-1 and ZO-1,natural OZP maintained the intestinal barrier function.Furthermore,natural OZP regulated the microbial composition by remarkedly enhancing the beneficial microbial abundance like Faecali-baculum,Odoribacter and Anaerostipes,which in turn led to a significant increase in SCFA production.Collec-tively,natural OZP exerted the synergistic capacity to protect against DSS-induced UC by improving antioxidation and anti-inflammation,repairing intestinal barrier and modulating intestinal microbiota and SCFA.展开更多
Metabolic diseases such as obesity and diabetes are increasing rapidly,leading to adverse effects on patients’intestinal function and health.In this study,high-purity conjugated linoleic acid(CLA)was extracted using ...Metabolic diseases such as obesity and diabetes are increasing rapidly,leading to adverse effects on patients’intestinal function and health.In this study,high-purity conjugated linoleic acid(CLA)was extracted using glycerol from Suaeda salsa,followed by purification using simulated moving bed chromatography technique.Subsequently,type 2 diabetic mellitus(T2DM)mice were administered c9,t11-CLA(100 mg/kg),t10,c12-CLA(100 mg/kg),or varying doses of high-purity CLA diet(100,600,or 1800 mg/kg)for a duration of five weeks.The impact on intestinal function was evaluated through morphological and tissue function studies combined with 16S rDNA sequencing technology and other techniques.The results demonstrated that the CLA was purified to 97.81%and significantly reduced blood glucose and lipid levels in T2DM mice by inhibiting the expression of small intestinal glucose and lipid transporters;enhanced colon barrier function by upregulating the expression of colon tight junction proteins;activated Jnk-c-Jun pathway to suppress intestinal cell apoptosis thereby improving survival rate;improved intestinal microecological health by modulating intestinal flora composition and metabolic function.These findings demonstrate the potential advantageous impact and mechanism of CLA derived from S.salsa in enhancing intestinal health among individuals with T2DM,providing a substantial theoretical and practical foundation for investigating the hypoglycemic effects of CLA and addressing global public health concerns.展开更多
We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus sp.PCC7002 during the lag,exponential,and stationary growth phas...We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus sp.PCC7002 during the lag,exponential,and stationary growth phases.Elevated p CO_2significantly stimulated growth,and enhanced the maximum cell density during the stationary phase.Under ambient p CO_2conditions,the lag phase lasted for 6 days,while elevated p CO_2shortened the lag phase to two days and extended the exponential phase by four days.The elevated p CO_2increased photosynthesis levels during the lag and exponential phases,but reduced them during the stationary phase.Moreover,the elevated p CO_2reduced the saturated growth light(Ik)and increased the light utilization efficiency(α)during the exponential and stationary phases,and elevated the phycobilisome:chlorophyll a(Chl a)ratio.Furthermore,the elevated p CO_2reduced the particulate organic carbon(POC):Chl a and particulate organic nitrogen(PON):Chl a ratios during the lag and stationary phases,but enhanced them during the exponential phase.Overall,Synechococcus showed differential physiological responses to elevated p CO_2during different growth phases,thus providing insight into previous studies that focused on only the exponential phase,which may have biased the results relative to the effects of elevated p CO_2in ecology or aquaculture.展开更多
基金The National Natural Science Foundation of China under contract Nos 32000074 and 42176130the Science and Technology Planning Project of Guangxi under contract No. AA21196002+4 种基金the Natural Science Foundation of Shandong Province under contract No. ZR2021MD044the Tai Mountain Industry Leading Talent of Shandong under contract No. 2019TSCYCX-06the Key Research and Development Program of Shandong Province under contract No. 2021TZXD008the Biosafety Research Program under contract No.20SWAQX04the Shandong Program of Pilot National Laboratory for Marine Science and Technology (Qingdao)under contract No. 2022QNLM030003-1。
文摘Microorganisms are fundamental for the functioning of marine ecosystems and are involved in the decomposition of organic matter, transformation of nutrients and circulation of biologically-important chemicals. Based on the complexity of the natural geographic characteristics of the Changjiang River Estuary, the geographic distribution of sedimentary microorganisms and the causes of this distribution are largely unexplored. In this work, the surface sediment samples from the adjacent sea area of the Changjiang River Estuary were collected. Their prokaryotic diversity was examined by high-throughput sequencing technology, and the environmental factors of the bacterial community were investigated. The results indicated that the distribution of prokaryotic communities in the sediments of the study areas showed obvious spatial heterogeneity. The sampling sequences divided the sample regions into three distinct clusters. Each geographic region had a unique community structure, although Proteobacteria, Bacteroidota, Desulfobacterota, Acidobacteriota, and Actinobacteriota all existed in these three branches. Canonical correspondence analysis demonstrated that prokaryotic diversity and community distribution were significantly correlated with the geographic location of sediment, seawater depth, and in particular, nutrient content(e.g., total phosphorus, total organic carbon and dissolved oxygen). Moreover, it was found for the first time that the metal ions obviously affected the composition and distribution of the prokaryotic community in this area. In general, this work provides new insights into the structural characteristics and driving factors of prokaryotic communities under the background of the ever-changing Changjiang River Estuary.
基金supported by the Innovation Team Program of Jinan(202228041)Natural Science Foundation of China(32470132,32000074,42176130)+3 种基金Natural Science Foundation of Shandong Prov-ince(ZR2021MD044)Key Research and Development Program of Shandong Province(2023TZXD047,2023CXPT087,2021TZXD008)Shandong Program of Qingdao Pilot National Laboratory for Marine Science and Technology(2022QNLM030003-1)Key Technology and Industrialization Demonstration Projects of Qingdao(23-1-4-xxgg-20-nsh,23-1-3hysf-1-hy,23-1-4-xxgg-13-nsh,22-3-5-yqpy-10-qy,24-1-3-hygg-24-hy,24-1-8-xdny-28-nsh).
文摘The 6-4 photolyase can specifically repair 6-pyrimidine-4-pyrimidinone(6-4 PP)photoproduct from UVBtriggered DNA damage.However,its application for skin damage hasn’t been reported yet.This study prepared the novel 6-4 photolyase nanoliposomes(6-4 NL),derived from UVB-resistant Antarctic Chlamydomonas sp.ICE-L,which effectively removed 6-4 PPs in UVB-induced skin.The 6-4 NL mitigated oxidative stress by remarkably reducing ROS,MDA and 8-OHdG,and enhancing SOD activity.Anti-inflammatory action was associated with the inhibition of NF-κB pathways by downregulating TNF-α,IL-6,COX-2 and NF-κB.The 6-4 NL regulated mitogen-activated protein kinase pathways via significantly diminishing expression of JNK,ERK and p38,and their phosphorylated forms.Additionally,anti-wrinkle effects were evidenced by significantly reducing the expression of AP-1,MMP-1 and MMP-2,and increasing hydroxyproline levels.These findings provided convincing evidence for the protective mechanisms of 6-4 NL against UVB-induced skin damage,as well as for the potential application in preventing photoaging and skin diseases.
基金funded by the National Key Research and Devel-opment Program of China(2023YFD2100604)Science and Technology Planning Project of Guangxi(AA21196002)+4 种基金Natural Science Foundation of China(32000074,42176130)Natural Science Foundation of Shandong(ZR2021MD044)Key Research and Development Program of Shandong(2023TZXD047,2023CXPT087,2021TZXD008)Key Technology and Industrialization Demonstration Projects of Qingdao(23-1-4-xxgg-20-nsh,23-1-3hysf-1-hy,23-1-4-xxgg-13-nsh,22-3-5-yqpy-10-qy,24-1-3-hygg-24-hy,24-1-8-xdny-28-nsh)Innovation Team Pro-gram of Jinan(202228041).
文摘Natural oyster zinc-rich peptide(OZP)was extracted and purified.The higher zinc concentration in natural OZP may be closely related to the high zinc affinity of amino acids containing Lys,His,Cys,Val,Arg and Ser residues.FTIR analysis indicated that amino,amide,carboxyl and carbonyl groups may contribute to the binding sites for zinc and peptides.Administration of OZP ameliorated the symptoms of DSS-induced ulcerative colitis(UC)in mice,including weight loss,colonic damage,mucosal edema,goblet cell exhaustion and crypt disappearance.Natural OZP suppressed oxidative stress by significantly reducing the level of NO,MDA and MPO as well as increasing SOD activity.The anti-inflammatory effects of OZP were associated with the inhibition of TLR4/NF-κB pathways by diminishing NF-κB,TLR4,COX-2,iNOS,TNF-α,IL-6 and IFN-γand elevating IL-10.By promoting mucus secretion,repairing goblet cells and enhancing the expression of tight junction proteins including occludin,claudin-1 and ZO-1,natural OZP maintained the intestinal barrier function.Furthermore,natural OZP regulated the microbial composition by remarkedly enhancing the beneficial microbial abundance like Faecali-baculum,Odoribacter and Anaerostipes,which in turn led to a significant increase in SCFA production.Collec-tively,natural OZP exerted the synergistic capacity to protect against DSS-induced UC by improving antioxidation and anti-inflammation,repairing intestinal barrier and modulating intestinal microbiota and SCFA.
基金funded by National Key Research and Development Program of China,grant number 2023YFD2100604Basic Scientific Fund for National Public Research Institutes of China,grant number 2022Q10+8 种基金Natural Science Foundation of China,grant number 42176130,32000074 and 42176242Natural Science Foundation of Shandong Province,grant number ZR2021MD044Taishan Industry Leading Talents,grant number 2019TSCYCX-06Natural Science Foundation of Qingdao,grant number 23-2-1-59-zyyd-jchKey Technology Research and Development Program of Shandong,grant number 2021TZXD008Qingdao Marine Science and Technology Center,grant number 2022QNLM030003-1Qingdao Key Technology Research and Industrialization Demonstration,grant number 23-1-4-xxgg-20-nsh,23-1-3hysf-1-hy,23-1-4-xxgg-13-nsh,22-3-5-yqpy-10-qyInnovation Team Program of Jinan,grant number 202228041Qingdao West Coast New Area Major Scientific and Technological Innovation Project,grant number ZDKC-2022-02.
文摘Metabolic diseases such as obesity and diabetes are increasing rapidly,leading to adverse effects on patients’intestinal function and health.In this study,high-purity conjugated linoleic acid(CLA)was extracted using glycerol from Suaeda salsa,followed by purification using simulated moving bed chromatography technique.Subsequently,type 2 diabetic mellitus(T2DM)mice were administered c9,t11-CLA(100 mg/kg),t10,c12-CLA(100 mg/kg),or varying doses of high-purity CLA diet(100,600,or 1800 mg/kg)for a duration of five weeks.The impact on intestinal function was evaluated through morphological and tissue function studies combined with 16S rDNA sequencing technology and other techniques.The results demonstrated that the CLA was purified to 97.81%and significantly reduced blood glucose and lipid levels in T2DM mice by inhibiting the expression of small intestinal glucose and lipid transporters;enhanced colon barrier function by upregulating the expression of colon tight junction proteins;activated Jnk-c-Jun pathway to suppress intestinal cell apoptosis thereby improving survival rate;improved intestinal microecological health by modulating intestinal flora composition and metabolic function.These findings demonstrate the potential advantageous impact and mechanism of CLA derived from S.salsa in enhancing intestinal health among individuals with T2DM,providing a substantial theoretical and practical foundation for investigating the hypoglycemic effects of CLA and addressing global public health concerns.
基金supported by the National Key Research and Development Program of China (Grant No. 2016YFA0601402)the China SOA Grant Associated with Task (Grant No. GASI-03-01-02-05)+1 种基金the CNOOC Zhanjiang Branch (Grant No. CNOOC-KJ 125 FZDXM 00 ZJ 001-2014)the National Natural Science Foundation of China (Grant Nos. 41606092 & 41676156)
文摘We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus sp.PCC7002 during the lag,exponential,and stationary growth phases.Elevated p CO_2significantly stimulated growth,and enhanced the maximum cell density during the stationary phase.Under ambient p CO_2conditions,the lag phase lasted for 6 days,while elevated p CO_2shortened the lag phase to two days and extended the exponential phase by four days.The elevated p CO_2increased photosynthesis levels during the lag and exponential phases,but reduced them during the stationary phase.Moreover,the elevated p CO_2reduced the saturated growth light(Ik)and increased the light utilization efficiency(α)during the exponential and stationary phases,and elevated the phycobilisome:chlorophyll a(Chl a)ratio.Furthermore,the elevated p CO_2reduced the particulate organic carbon(POC):Chl a and particulate organic nitrogen(PON):Chl a ratios during the lag and stationary phases,but enhanced them during the exponential phase.Overall,Synechococcus showed differential physiological responses to elevated p CO_2during different growth phases,thus providing insight into previous studies that focused on only the exponential phase,which may have biased the results relative to the effects of elevated p CO_2in ecology or aquaculture.
