摘要
The 6-4 photolyase can specifically repair 6-pyrimidine-4-pyrimidinone(6-4 PP)photoproduct from UVBtriggered DNA damage.However,its application for skin damage hasn’t been reported yet.This study prepared the novel 6-4 photolyase nanoliposomes(6-4 NL),derived from UVB-resistant Antarctic Chlamydomonas sp.ICE-L,which effectively removed 6-4 PPs in UVB-induced skin.The 6-4 NL mitigated oxidative stress by remarkably reducing ROS,MDA and 8-OHdG,and enhancing SOD activity.Anti-inflammatory action was associated with the inhibition of NF-κB pathways by downregulating TNF-α,IL-6,COX-2 and NF-κB.The 6-4 NL regulated mitogen-activated protein kinase pathways via significantly diminishing expression of JNK,ERK and p38,and their phosphorylated forms.Additionally,anti-wrinkle effects were evidenced by significantly reducing the expression of AP-1,MMP-1 and MMP-2,and increasing hydroxyproline levels.These findings provided convincing evidence for the protective mechanisms of 6-4 NL against UVB-induced skin damage,as well as for the potential application in preventing photoaging and skin diseases.
基金
supported by the Innovation Team Program of Jinan(202228041)
Natural Science Foundation of China(32470132,32000074,42176130)
Natural Science Foundation of Shandong Prov-ince(ZR2021MD044)
Key Research and Development Program of Shandong Province(2023TZXD047,2023CXPT087,2021TZXD008)
Shandong Program of Qingdao Pilot National Laboratory for Marine Science and Technology(2022QNLM030003-1)
Key Technology and Industrialization Demonstration Projects of Qingdao(23-1-4-xxgg-20-nsh,23-1-3hysf-1-hy,23-1-4-xxgg-13-nsh,22-3-5-yqpy-10-qy,24-1-3-hygg-24-hy,24-1-8-xdny-28-nsh).
