Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold ...Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold molding process with 20%w/v poly-vinylpyrrolidone and 15%w/v polyvinyl alcohol.The SFA-MNs were assessed for morphology,mechanical properties,in vitro dissolution,identification of components,and skin lesion improvement in imiquimod-induced psoriasis mice.Results:The SFA-MNs demonstrated good mechanical properties for efficiently penetrating the dermis,facilitating efficient drug delivery.Furthermore,they effectively inhibited mast cell levels in the dorsal lesion area of psoriasis mice and reduced the expression of the T-lymphocyte factor cluster of differ-entiation 3 and tumor necrosis factor-a.In addition,this system alleviated skin inflammation,splenic swelling,and thymic atrophy in the psoriasis-like mouse model.Seven major components were detected from SFA-MNs by comparison of the mass-to-nucleus ratios(m/z)of the secondary fragments N-methylcytisine,5a,9a-dihydroxymatrine,sophoramine,matrine,oxysophocarpine,oxymatrine,and kushenol O.Conclusion:The drug delivery strategy combining traditional herbal S.flavescens with soluble micro-needle technology provides more targeted and effective immune regulation for treating psoriasis-like mice models,enabling enhanced therapeutic effects compared with the control group.展开更多
背景:拇外翻是临床常见足部畸形之一,严重影响患者生活质量,传统手术治疗采取大切口,直视下矫形手术,患者痛苦大,恢复慢。随着微创技术的进步与发展,中西医结合微创治疗拇外翻在临床应用广泛,具有切口小,恢复快,能够尽早下地等优点。目...背景:拇外翻是临床常见足部畸形之一,严重影响患者生活质量,传统手术治疗采取大切口,直视下矫形手术,患者痛苦大,恢复慢。随着微创技术的进步与发展,中西医结合微创治疗拇外翻在临床应用广泛,具有切口小,恢复快,能够尽早下地等优点。目的:测量拇外翻微创截骨治疗后"8"字绷带外固定患者行走、站立及主被动功能锻炼时截骨端周围压力。方法:对1例拇外翻微创治疗后4周的24岁女性患者,运用SPI FREE FORM 32通道单点压力测量系统测量患者在行走、站立、主动跖屈、主动背伸、被动跖屈与被动背伸6种状态下"8"字绷带外固定拇外翻截骨端周围的压力参数。结果与结论:通过对拇外翻治疗后"8"字绷带外固定下不同区域的压力分析,趾蹼正中始终保持较大的压力;除站立状态下,跖骨头背侧压力均大于跖骨头下压力;除被动跖屈状态下,趾蹼侧方压力均大于跖骨头内侧压力。提示拇外翻微创截骨治疗后"8"字绷带外固定能维持截骨远端跖屈位及拇趾中立位,有效保持截骨端的稳定,有利于骨折愈合。展开更多
基金supported by the National Natural Science Foundation of China(82274225)NATCM's Project of High-level Construction of Key TCM Disciplines-Beijing University of Chinese Medicine-Life Science from the Perspective of Chinese Medicine(zyyzdxk-2023263).
文摘Objective:To assess the efficiency of a Sophora flavescens Ait(S.flavescens,Ku Shen)-soluble microneedle(SFA-MN)for improving skin lesion symptoms in mice with psoriasis.Methods:SFA-MNs were prepared using a two-mold molding process with 20%w/v poly-vinylpyrrolidone and 15%w/v polyvinyl alcohol.The SFA-MNs were assessed for morphology,mechanical properties,in vitro dissolution,identification of components,and skin lesion improvement in imiquimod-induced psoriasis mice.Results:The SFA-MNs demonstrated good mechanical properties for efficiently penetrating the dermis,facilitating efficient drug delivery.Furthermore,they effectively inhibited mast cell levels in the dorsal lesion area of psoriasis mice and reduced the expression of the T-lymphocyte factor cluster of differ-entiation 3 and tumor necrosis factor-a.In addition,this system alleviated skin inflammation,splenic swelling,and thymic atrophy in the psoriasis-like mouse model.Seven major components were detected from SFA-MNs by comparison of the mass-to-nucleus ratios(m/z)of the secondary fragments N-methylcytisine,5a,9a-dihydroxymatrine,sophoramine,matrine,oxysophocarpine,oxymatrine,and kushenol O.Conclusion:The drug delivery strategy combining traditional herbal S.flavescens with soluble micro-needle technology provides more targeted and effective immune regulation for treating psoriasis-like mice models,enabling enhanced therapeutic effects compared with the control group.
文摘背景:拇外翻是临床常见足部畸形之一,严重影响患者生活质量,传统手术治疗采取大切口,直视下矫形手术,患者痛苦大,恢复慢。随着微创技术的进步与发展,中西医结合微创治疗拇外翻在临床应用广泛,具有切口小,恢复快,能够尽早下地等优点。目的:测量拇外翻微创截骨治疗后"8"字绷带外固定患者行走、站立及主被动功能锻炼时截骨端周围压力。方法:对1例拇外翻微创治疗后4周的24岁女性患者,运用SPI FREE FORM 32通道单点压力测量系统测量患者在行走、站立、主动跖屈、主动背伸、被动跖屈与被动背伸6种状态下"8"字绷带外固定拇外翻截骨端周围的压力参数。结果与结论:通过对拇外翻治疗后"8"字绷带外固定下不同区域的压力分析,趾蹼正中始终保持较大的压力;除站立状态下,跖骨头背侧压力均大于跖骨头下压力;除被动跖屈状态下,趾蹼侧方压力均大于跖骨头内侧压力。提示拇外翻微创截骨治疗后"8"字绷带外固定能维持截骨远端跖屈位及拇趾中立位,有效保持截骨端的稳定,有利于骨折愈合。
