Development of photocatalytic materials able to profit from the whole spectrum of sun for efficient organic pollution removal and bacterial elimination is a fascinating strategy in environmental engineering.In current...Development of photocatalytic materials able to profit from the whole spectrum of sun for efficient organic pollution removal and bacterial elimination is a fascinating strategy in environmental engineering.In current work,GO-TiO_(2)/(Ca,Y)F_(2):Tm,Yb,a full-spectrum composite photocatalyst,was prepared via hydrothermal processing with TiO_(2)/(Ca,Y)F_(2):Tm,Yb particles deposited on the surface of graphene oxide(GO).The surface properties of GO-TiO_(2)/(Ca,Y)F_(2):Tm,Yb were characterized using various characterization techniques including X-ray diffraction(XRD),scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS)and Brunauer–Emmett–Teller(BET)surface area analyzer.Besides,ultraviolet–visible(UV–Vis)diffuse reflectance spectroscopy,electron spin resonance(ESR)and other methods were used to systematically explore the photocatalytic mechanism of GO enhancement of TiO_(2)/(Ca,Y)F_(2):Tm,Yb.In photocatalytic degradation study,the degradation rate of methyl orange(MO)was improved by 40%when GO was added into the TiO_(2)/(Ca,Y)F_(2):Tm,Yb system.In addition,the presence of GO also improves the antibacterial ability of TiO_(2)/(Ca,Y)F_(2):Tm,Yb against Escherichia coli and Staphylococcus aureus.All results reveal high efficacy of GOTiO_(2)/(Ca,Y)F_(2):Tm,Yb under full spectrum light irradiation,in which(Ca,Y)F2:Tm,Yb and TiO2 can improve its utilization of full spectrum sunlight,and GO can enhance adsorption ability of dye and electron–hole pair differentiation ability of TiO_(2)/(Ca,Y)F_(2):Tm,Yb.展开更多
An algorithm capable of predicting and optimizing the gradient separation of LC×LC system was developed in this paper.Two groups of structural analogues,five ginsenosides as well as eight bisphenols,which were di...An algorithm capable of predicting and optimizing the gradient separation of LC×LC system was developed in this paper.Two groups of structural analogues,five ginsenosides as well as eight bisphenols,which were difficult to discriminate in routine analysis,were used to verify the effectiveness of the proposed algorithm in fast separation optimization.Average errors of retention times below 1%were found in the retention prediction for all types of gradient programs,implying that the theory could lead to high quality in prediction of the retention times under gradients elution.Meanwhile,84%of relative average deviations(RADs)between the predicted peak width and the measured ones were less than 20%.The larger deviation occurred at the time when the peak appeared while the gradient of the mobile phase changed,which led the deviations increased to 20%–42%.In all,method development and optimization for LC×LC tandem system was realized by the homemade user-friendly software.The present protocol may turn on great opportunities for the convenient method development in analysis of trace structural analogues in environmental,food and biological samples.展开更多
Homologous booster,heterologous booster,and Omicron variants breakthrough infection(OBI)could improve the humoral immunity against Omicron variants.Questions concerning about memory B cells(MBCs)and T cells immunity a...Homologous booster,heterologous booster,and Omicron variants breakthrough infection(OBI)could improve the humoral immunity against Omicron variants.Questions concerning about memory B cells(MBCs)and T cells immunity against Omicron variants,features of long-term immunity,after booster and OBI,needs to be explored.Here,comparative analysis demonstrate antibody and T cell immunity against ancestral strain,Delta and Omicron variants in Omicron breakthrough infected patients(OBIPs)are comparable to that in Ad5-nCoV boosted healthy volunteers(HVs),higher than that in inactivated vaccine(InV)boosted HVs.However,memory B cells(MBCs)immunity against Omicron variants was highest in OBIPs,followed by Ad5-nCoV boosted and InV boosted HVs.OBIPs and Ad5-nCoV boosted HVs have higher classical MBCs and activated MBCs,and lower naïve MBCs and atypical MBCs relative to both vaccine boosted HVs.Collectively,these data indicate Omicron breakthrough infection elicit higher MBCs and T cells against SARS-CoV-2 especially Omicron variants relative to homologous InV booster and heterologous Ad5-nCoV booster.展开更多
本研究以8个产地的珊瑚菜(Glehnia littoralis Fr.Schmidt ex Miq.)为材料,通过根尖染色体压片观察研究了染色体数目、核型,并通过巢式方差分析,在常规染色体水平上探讨了居群间、居群内个体间染色体大小和形态的变异情况。结果表明:8...本研究以8个产地的珊瑚菜(Glehnia littoralis Fr.Schmidt ex Miq.)为材料,通过根尖染色体压片观察研究了染色体数目、核型,并通过巢式方差分析,在常规染色体水平上探讨了居群间、居群内个体间染色体大小和形态的变异情况。结果表明:8个产地珊瑚菜体细胞染色体数目均为2n=2x=22条。其核型公式多样化,即墨、海阳、瓦房店情人岛、赤峰的分别为2n=2x=22=18m+4sm、2n=2x=22=12m+10sm、2n=2x=22=16m+6sm和2n=2x=22=14m+8sm,莱阳、日照、安国、瓦房店八仙岛的均为2n=2x=22=20m+2sm,仅有中部着丝点(m)型和近中部着丝点(sm)型染色体,包含短染色体(S)、中短染色体(M1)、中长染色体(M2)、长染色体(L)4种长度类型。4类核型均为较原始的类型,莱阳、日照、瓦房店情人岛的为“1A”型、海阳、即墨、瓦房店八仙岛的为“2A”型、安国和赤峰的分别为“1B”型和“2B”型。染色体长度比值在各个产地珊瑚菜中介于1.46~2.79之间,核型不对称系数范围在57.36%~60.29%。珊瑚菜染色体变异主要来源于居群内,染色体相对长度和臂比的方差分量百分比分别为69.41%和97.09%。该研究结果丰富了珊瑚菜细胞学方面的信息,初步揭示了不同产地珊瑚菜之间的变异情况,为进一步的选种育种以及揭示珊瑚菜属的遗传、进化机制提供理论基础。展开更多
基金This work was financially supported by the National Key Research and Development Program of China(No.2020YFC1107403)the National Natural Science Foundation of China(No.51771069)the Natural Science Foundation of Hebei Province of China(No.E2020202028).
文摘Development of photocatalytic materials able to profit from the whole spectrum of sun for efficient organic pollution removal and bacterial elimination is a fascinating strategy in environmental engineering.In current work,GO-TiO_(2)/(Ca,Y)F_(2):Tm,Yb,a full-spectrum composite photocatalyst,was prepared via hydrothermal processing with TiO_(2)/(Ca,Y)F_(2):Tm,Yb particles deposited on the surface of graphene oxide(GO).The surface properties of GO-TiO_(2)/(Ca,Y)F_(2):Tm,Yb were characterized using various characterization techniques including X-ray diffraction(XRD),scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS)and Brunauer–Emmett–Teller(BET)surface area analyzer.Besides,ultraviolet–visible(UV–Vis)diffuse reflectance spectroscopy,electron spin resonance(ESR)and other methods were used to systematically explore the photocatalytic mechanism of GO enhancement of TiO_(2)/(Ca,Y)F_(2):Tm,Yb.In photocatalytic degradation study,the degradation rate of methyl orange(MO)was improved by 40%when GO was added into the TiO_(2)/(Ca,Y)F_(2):Tm,Yb system.In addition,the presence of GO also improves the antibacterial ability of TiO_(2)/(Ca,Y)F_(2):Tm,Yb against Escherichia coli and Staphylococcus aureus.All results reveal high efficacy of GOTiO_(2)/(Ca,Y)F_(2):Tm,Yb under full spectrum light irradiation,in which(Ca,Y)F2:Tm,Yb and TiO2 can improve its utilization of full spectrum sunlight,and GO can enhance adsorption ability of dye and electron–hole pair differentiation ability of TiO_(2)/(Ca,Y)F_(2):Tm,Yb.
基金supported by National Natural Science Foundation of China(Nos.82174090,22176085,21874065,21577057)Natural Science Foundation for Colleges of Jiangsu(No.21KJA360007)+2 种基金Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)Jiangsu Qinglan ProjectJiangsu“333”Project。
文摘An algorithm capable of predicting and optimizing the gradient separation of LC×LC system was developed in this paper.Two groups of structural analogues,five ginsenosides as well as eight bisphenols,which were difficult to discriminate in routine analysis,were used to verify the effectiveness of the proposed algorithm in fast separation optimization.Average errors of retention times below 1%were found in the retention prediction for all types of gradient programs,implying that the theory could lead to high quality in prediction of the retention times under gradients elution.Meanwhile,84%of relative average deviations(RADs)between the predicted peak width and the measured ones were less than 20%.The larger deviation occurred at the time when the peak appeared while the gradient of the mobile phase changed,which led the deviations increased to 20%–42%.In all,method development and optimization for LC×LC tandem system was realized by the homemade user-friendly software.The present protocol may turn on great opportunities for the convenient method development in analysis of trace structural analogues in environmental,food and biological samples.
基金Guangzhou Health Science and Technology Project(20201A011078)Guangzhou Science and Technology Project(202102010094)+5 种基金Guangdong Basic and Applied Basic Research Foundation(2021A1515012550)Clinical research project of Guangzhou Medical University Second Affiliated Hospital(2021-LCYJ-05)Guangdong Medical Research Fund(A2022255)Key Clinical Specialty of Guangzhou Medical University(0F03031)Guangzhou Laboratory(EKPG21-30-3)Guangzhou key discipline of urology.The funding sources had no role in the study design,data collection,analysis,interpretation,or writing of the report.
文摘Homologous booster,heterologous booster,and Omicron variants breakthrough infection(OBI)could improve the humoral immunity against Omicron variants.Questions concerning about memory B cells(MBCs)and T cells immunity against Omicron variants,features of long-term immunity,after booster and OBI,needs to be explored.Here,comparative analysis demonstrate antibody and T cell immunity against ancestral strain,Delta and Omicron variants in Omicron breakthrough infected patients(OBIPs)are comparable to that in Ad5-nCoV boosted healthy volunteers(HVs),higher than that in inactivated vaccine(InV)boosted HVs.However,memory B cells(MBCs)immunity against Omicron variants was highest in OBIPs,followed by Ad5-nCoV boosted and InV boosted HVs.OBIPs and Ad5-nCoV boosted HVs have higher classical MBCs and activated MBCs,and lower naïve MBCs and atypical MBCs relative to both vaccine boosted HVs.Collectively,these data indicate Omicron breakthrough infection elicit higher MBCs and T cells against SARS-CoV-2 especially Omicron variants relative to homologous InV booster and heterologous Ad5-nCoV booster.
文摘本研究以8个产地的珊瑚菜(Glehnia littoralis Fr.Schmidt ex Miq.)为材料,通过根尖染色体压片观察研究了染色体数目、核型,并通过巢式方差分析,在常规染色体水平上探讨了居群间、居群内个体间染色体大小和形态的变异情况。结果表明:8个产地珊瑚菜体细胞染色体数目均为2n=2x=22条。其核型公式多样化,即墨、海阳、瓦房店情人岛、赤峰的分别为2n=2x=22=18m+4sm、2n=2x=22=12m+10sm、2n=2x=22=16m+6sm和2n=2x=22=14m+8sm,莱阳、日照、安国、瓦房店八仙岛的均为2n=2x=22=20m+2sm,仅有中部着丝点(m)型和近中部着丝点(sm)型染色体,包含短染色体(S)、中短染色体(M1)、中长染色体(M2)、长染色体(L)4种长度类型。4类核型均为较原始的类型,莱阳、日照、瓦房店情人岛的为“1A”型、海阳、即墨、瓦房店八仙岛的为“2A”型、安国和赤峰的分别为“1B”型和“2B”型。染色体长度比值在各个产地珊瑚菜中介于1.46~2.79之间,核型不对称系数范围在57.36%~60.29%。珊瑚菜染色体变异主要来源于居群内,染色体相对长度和臂比的方差分量百分比分别为69.41%和97.09%。该研究结果丰富了珊瑚菜细胞学方面的信息,初步揭示了不同产地珊瑚菜之间的变异情况,为进一步的选种育种以及揭示珊瑚菜属的遗传、进化机制提供理论基础。
