Objective To observe the effect of electroacupuncture at ear and body acupoints for the instant fasting blood glucose of model machins with type 2 diabetes mellitus（T2DM）.Methods For 10 machins with type2 diabetes,t...Objective To observe the effect of electroacupuncture at ear and body acupoints for the instant fasting blood glucose of model machins with type 2 diabetes mellitus（T2DM）.Methods For 10 machins with type2 diabetes,the electroacupuncture was applied at ＂Zusanli＂（足三里 ST 36）,＂Sanyinjiao＂（三阴交 SP 6） and three ＂Jiangtangdian＂（降糖点） in the ear continuously for 5 days,with the frequency of 2/15 Hz（The 2 Hz and the 15 Hz were transferred every second） and current of2 mA.The treatment was given once every day for 30 min.The fasting blood glucose was measured before acupuncture,after acupuncture for15 min and 30 min in the 5 days.Results On the first day,after 30-minute acupuncture the fasting blood glucose was reduced obviously compared with that before treatment（P〈0.01）.On the third and the fifth days,after acupuncture for 15 min and 30 min the fasting blood glucose was apparently lower than that before acupuncture（all P〈0.05）.On the fourth day,after acupuncture for 30 min the fasting blood glucose was decreased markedly than that before acupuncture（P〈0.05）.After 30-minute acupuncture on the fifth day,the fasting blood glucose was lower obviously than that before treatment on the first day（P〈0.05）.Conclusion ①The electroacupuncture at ear and body acupoints can reduce the instant fasting blood glucose of T2 DM machins apparently.② There is time effect on instant fasting blood glucose of T2 DM machins treated by electroacupuncture.The efficacy of 30-minute stimulation is superior to that of 15-minute acupuncture.After acupuncture continuously for 4 days,the effect is better than that with acupuncture for3 days successively.展开更多
目的:观察经皮耳穴-迷走神经刺激(taVNS)对糖耐量受损(IGT)模型大鼠血糖调节及下丘脑、肝脏、骨骼肌胰岛素受体(INR)表达的影响,探讨taVNS改善IGT的作用机制。方法:36只雄性Wistar大鼠随机分为正常组、模型组、耳缘-非迷走神经刺激(tnV...目的:观察经皮耳穴-迷走神经刺激(taVNS)对糖耐量受损(IGT)模型大鼠血糖调节及下丘脑、肝脏、骨骼肌胰岛素受体(INR)表达的影响,探讨taVNS改善IGT的作用机制。方法:36只雄性Wistar大鼠随机分为正常组、模型组、耳缘-非迷走神经刺激(tnVNS)组、耳穴-迷走神经刺激(taVNS)组,每组9只。正常组予普通饲料喂养,模型组、tnVNS组和taVNS组予高糖高脂饲料喂养联合腹腔注射小剂量链尿佐菌素以制备IGT模型。造模后对tnVNS组和taVNS组大鼠连续干预4周,tnVNS组刺激部位为耳缘区(无迷走神经分布)、taVNS组刺激部位为耳甲区(有迷走神经分布),强度为2 mA,频率为疏密波2 Hz/15 Hz,每次30 min,每天1次,共4周。整个实验持续10周,记录各组大鼠的体质量、空腹血糖(FPG)、餐后2 h血糖(2 h PG),ELISA法,检测血浆胰岛素(INS)、胰高血糖素(GC)、糖化血红蛋白(GHbA1c)水平,Western blot法检测INR在下丘脑、肝脏、骨骼肌中的表达量。结果:造模后与正常组比较,其余3组大鼠体质量均明显增加(P<0.001),FPG和2 h PG升高(P<0.05,P<0.001),符合IGT的诊断标准,提示造模成功。干预后与正常组比较,模型组大鼠体质量明显增加(P<0.001),FPG、2 h PG及GC、GHbA1c含量明显升高(P<0.05,P<0.01),血浆INS含量及下丘脑、肝脏、骨骼肌INR表达明显降低(P<0.001,P<0.01,P<0.05)。干预后与模型组比较,taVNS组大鼠体质量、FPG、2 h PG、血浆GC含量明显降低(P<0.01,P<0.05),INS含量及下丘脑、肝脏、骨骼肌中INR表达明显升高(P<0.001,P<0.01,P<0.05)。干预后与tnVNS组比较,taVNS组大鼠FPG和2 h PG值降低(P<0.05),INS含量及下丘脑、肝脏中INR表达明显升高(P<0.001,P<0.05,P<0.01)。结论:经皮耳穴-迷走神经刺激可调节IGT模型大鼠的FPG和2 h PG,降低GC,提高INS敏感性,上调下丘脑、肝脏、骨骼肌中INR的表达。展开更多
基金National Basic Research Program of China:2012CB518503National twelfth five-year science and technology support program:2012BAF14B10Special fund of technology research and development for institute by Ministry of science and Technology of China:2011EG152313
文摘Objective To observe the effect of electroacupuncture at ear and body acupoints for the instant fasting blood glucose of model machins with type 2 diabetes mellitus（T2DM）.Methods For 10 machins with type2 diabetes,the electroacupuncture was applied at ＂Zusanli＂（足三里 ST 36）,＂Sanyinjiao＂（三阴交 SP 6） and three ＂Jiangtangdian＂（降糖点） in the ear continuously for 5 days,with the frequency of 2/15 Hz（The 2 Hz and the 15 Hz were transferred every second） and current of2 mA.The treatment was given once every day for 30 min.The fasting blood glucose was measured before acupuncture,after acupuncture for15 min and 30 min in the 5 days.Results On the first day,after 30-minute acupuncture the fasting blood glucose was reduced obviously compared with that before treatment（P〈0.01）.On the third and the fifth days,after acupuncture for 15 min and 30 min the fasting blood glucose was apparently lower than that before acupuncture（all P〈0.05）.On the fourth day,after acupuncture for 30 min the fasting blood glucose was decreased markedly than that before acupuncture（P〈0.05）.After 30-minute acupuncture on the fifth day,the fasting blood glucose was lower obviously than that before treatment on the first day（P〈0.05）.Conclusion ①The electroacupuncture at ear and body acupoints can reduce the instant fasting blood glucose of T2 DM machins apparently.② There is time effect on instant fasting blood glucose of T2 DM machins treated by electroacupuncture.The efficacy of 30-minute stimulation is superior to that of 15-minute acupuncture.After acupuncture continuously for 4 days,the effect is better than that with acupuncture for3 days successively.
文摘目的:观察经皮耳穴-迷走神经刺激(taVNS)对糖耐量受损(IGT)模型大鼠血糖调节及下丘脑、肝脏、骨骼肌胰岛素受体(INR)表达的影响,探讨taVNS改善IGT的作用机制。方法:36只雄性Wistar大鼠随机分为正常组、模型组、耳缘-非迷走神经刺激(tnVNS)组、耳穴-迷走神经刺激(taVNS)组,每组9只。正常组予普通饲料喂养,模型组、tnVNS组和taVNS组予高糖高脂饲料喂养联合腹腔注射小剂量链尿佐菌素以制备IGT模型。造模后对tnVNS组和taVNS组大鼠连续干预4周,tnVNS组刺激部位为耳缘区(无迷走神经分布)、taVNS组刺激部位为耳甲区(有迷走神经分布),强度为2 mA,频率为疏密波2 Hz/15 Hz,每次30 min,每天1次,共4周。整个实验持续10周,记录各组大鼠的体质量、空腹血糖(FPG)、餐后2 h血糖(2 h PG),ELISA法,检测血浆胰岛素(INS)、胰高血糖素(GC)、糖化血红蛋白(GHbA1c)水平,Western blot法检测INR在下丘脑、肝脏、骨骼肌中的表达量。结果:造模后与正常组比较,其余3组大鼠体质量均明显增加(P<0.001),FPG和2 h PG升高(P<0.05,P<0.001),符合IGT的诊断标准,提示造模成功。干预后与正常组比较,模型组大鼠体质量明显增加(P<0.001),FPG、2 h PG及GC、GHbA1c含量明显升高(P<0.05,P<0.01),血浆INS含量及下丘脑、肝脏、骨骼肌INR表达明显降低(P<0.001,P<0.01,P<0.05)。干预后与模型组比较,taVNS组大鼠体质量、FPG、2 h PG、血浆GC含量明显降低(P<0.01,P<0.05),INS含量及下丘脑、肝脏、骨骼肌中INR表达明显升高(P<0.001,P<0.01,P<0.05)。干预后与tnVNS组比较,taVNS组大鼠FPG和2 h PG值降低(P<0.05),INS含量及下丘脑、肝脏中INR表达明显升高(P<0.001,P<0.05,P<0.01)。结论:经皮耳穴-迷走神经刺激可调节IGT模型大鼠的FPG和2 h PG,降低GC,提高INS敏感性,上调下丘脑、肝脏、骨骼肌中INR的表达。
