Tobacco and potato samples showing symptoms of PVY were collected from different regions in Anhui Province,and the ELISA results of partial samples were positive.The total RNA was extracted from the positive samples b...Tobacco and potato samples showing symptoms of PVY were collected from different regions in Anhui Province,and the ELISA results of partial samples were positive.The total RNA was extracted from the positive samples by TRIZOL methods.Specific primer pair was designed to amplify cp gene of PVY by RT-PCR.Sequencing results indicated that the full length of cp gene of PVY from tobacco(PVY-CP-4) and potato(PVY-CP-7) is 801 nts,and each of them encodes 266 amino acids.A phylogenetic tree based on alignment of cp nucleotide sequences was constructed and the sequence comparing of cp gene was conducted.The results showed that PVY-CP-4 could be grouped into one branch with PVYO and PVYN:O and shared the highest sequence similarity(99.4%) with PVYO(EF026074).It was suggested that PVY-CP-4 derived from tobacco in Hefei might belong to PVYO.PVY-CP-7 clustered together with PVYN and PVYNTN and formed another branch.Furthermore,PVY-CP-7 shared the highest sequence similarity(98.3%) with PVYNTN(AJ890347),PVYNTN(EF026075) and PVYNTN(AJ585342).It was supposed that PVY-CP-7 derived from potato in Wuhe probably belonged to PVYNTN.展开更多
文摘Tobacco and potato samples showing symptoms of PVY were collected from different regions in Anhui Province,and the ELISA results of partial samples were positive.The total RNA was extracted from the positive samples by TRIZOL methods.Specific primer pair was designed to amplify cp gene of PVY by RT-PCR.Sequencing results indicated that the full length of cp gene of PVY from tobacco(PVY-CP-4) and potato(PVY-CP-7) is 801 nts,and each of them encodes 266 amino acids.A phylogenetic tree based on alignment of cp nucleotide sequences was constructed and the sequence comparing of cp gene was conducted.The results showed that PVY-CP-4 could be grouped into one branch with PVYO and PVYN:O and shared the highest sequence similarity(99.4%) with PVYO(EF026074).It was suggested that PVY-CP-4 derived from tobacco in Hefei might belong to PVYO.PVY-CP-7 clustered together with PVYN and PVYNTN and formed another branch.Furthermore,PVY-CP-7 shared the highest sequence similarity(98.3%) with PVYNTN(AJ890347),PVYNTN(EF026075) and PVYNTN(AJ585342).It was supposed that PVY-CP-7 derived from potato in Wuhe probably belonged to PVYNTN.
