摘要
Tobacco and potato samples showing symptoms of PVY were collected from different regions in Anhui Province,and the ELISA results of partial samples were positive.The total RNA was extracted from the positive samples by TRIZOL methods.Specific primer pair was designed to amplify cp gene of PVY by RT-PCR.Sequencing results indicated that the full length of cp gene of PVY from tobacco(PVY-CP-4) and potato(PVY-CP-7) is 801 nts,and each of them encodes 266 amino acids.A phylogenetic tree based on alignment of cp nucleotide sequences was constructed and the sequence comparing of cp gene was conducted.The results showed that PVY-CP-4 could be grouped into one branch with PVYO and PVYN:O and shared the highest sequence similarity(99.4%) with PVYO(EF026074).It was suggested that PVY-CP-4 derived from tobacco in Hefei might belong to PVYO.PVY-CP-7 clustered together with PVYN and PVYNTN and formed another branch.Furthermore,PVY-CP-7 shared the highest sequence similarity(98.3%) with PVYNTN(AJ890347),PVYNTN(EF026075) and PVYNTN(AJ585342).It was supposed that PVY-CP-7 derived from potato in Wuhe probably belonged to PVYNTN.
Tobacco and potato samples showing symptoms of PVY were collected from different regions in Anhui Province, and the ELISA results of partial samples were positive. The total RNA was extracted from the positive samples by TRIZOL methods. Specific primer pair was designed to amplify cp gene of PVY by RT- PCR. Sequencing results indicated that the full length of cp gene of PVY from tobacco （PVY-CP-4） and pota-to （PVY-CP-7） is 801 nts, and each of them encodes 266 amino acids. A phylogenetic tree based on alignment of cp nucleotide sequences was constructed and the sequence comparing of cp gene was conducted. The results showed that PVY-CP-4 could be grouped into one branch with PVY^O and PVY^N：O and shared the highest sequence similarity （99.4%） with PVY^O （EF026074）. It was suggested that PVY-CP-4 derived from tobacco in Hefei might belong to PVY^O. PVY-CP-7 clustered together with PVYN and PVYNTN and formed another branch. Furthermore, PVY-CP-7 shared the highest sequence similarity （98.3%） with PVY^NTN（ AJ890347 ）, pvyNTN （EF026075） and pvyNTN（AJ585342）. It was supposed that PVY-CP-7 derived from potato in Wuhe probably belonged to PVYNTN.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第5期540-543,共4页
Acta Phytopathologica Sinica
基金
安徽省自然科学基金项目(070411002)
安徽省教育厅自然科学基金项目(KJ2007B121)
