目的:探讨虎黄洗剂对糖尿病创面愈合的影响及作用机制。方法:通过中药系统药理学数据库分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)数据库查询虎黄洗剂各组分的有效成分及靶基...目的:探讨虎黄洗剂对糖尿病创面愈合的影响及作用机制。方法:通过中药系统药理学数据库分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)数据库查询虎黄洗剂各组分的有效成分及靶基因。通过比较毒理基因组学数据库(Comparative Toxicogenomics Database,CTD)及GeneCards数据库筛选糖尿病足疾病靶点。采用Cytoscape软件构建成分-靶点相互作用网络。使用DAVID在线工具进行基因本体(gene ontology,GO)及京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。对糖尿病大鼠创面组织采用转录组学测序分析。动物实验验证虎黄洗剂对糖尿病大鼠创面愈合的影响,酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测创面组织炎症因子白细胞介素(interleukin,IL)-6、肿瘤坏死因子(tumor necrosis factor,TNF)-α及IL-1β表达。结果:网络药理学及转录组学测序分析表明,虎黄洗剂潜在靶点与炎症调控密切相关。创面局部应用虎黄洗剂可促进糖尿病创面愈合,苏木精-伊红(hematoxylin and eosin,HE)染色结果显示虎黄洗剂可促进糖尿病创面上皮化,ELISA检测结果提示虎黄洗剂可抑制创面组织炎症因子IL-6、TNF-α及IL-1β表达。结论:虎黄洗剂可抑制糖尿病创面炎症反应,促进创面愈合。展开更多
This paper reports that four new glycosyl esters of Vanillic Acid were synthesized by the reaction of vanillic acid with O-acetylglucopyranosyl bromide or O-acetylgalacopyranosyl bromide,O-acetylxylopyranosyl bromide ...This paper reports that four new glycosyl esters of Vanillic Acid were synthesized by the reaction of vanillic acid with O-acetylglucopyranosyl bromide or O-acetylgalacopyranosyl bromide,O-acetylxylopyranosyl bromide and O-acetyllactosyl bromide in the presence of Ag2O respectively;and vanillic acid-4-β-D-2’,3’,4’,6’-acetyl glucoside-β-D-2",3",4",6"-acetyl glucoester by vanillic acid with O-acetylglucopyranosyl bromide in the presence of Bu4NBr.Their structures were confirmed by IR,1H NMR,13C NMR,HMBC and MS.And spectra data proved that the products are of β-configuration.展开更多
文摘目的:探讨虎黄洗剂对糖尿病创面愈合的影响及作用机制。方法:通过中药系统药理学数据库分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)数据库查询虎黄洗剂各组分的有效成分及靶基因。通过比较毒理基因组学数据库(Comparative Toxicogenomics Database,CTD)及GeneCards数据库筛选糖尿病足疾病靶点。采用Cytoscape软件构建成分-靶点相互作用网络。使用DAVID在线工具进行基因本体(gene ontology,GO)及京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。对糖尿病大鼠创面组织采用转录组学测序分析。动物实验验证虎黄洗剂对糖尿病大鼠创面愈合的影响,酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测创面组织炎症因子白细胞介素(interleukin,IL)-6、肿瘤坏死因子(tumor necrosis factor,TNF)-α及IL-1β表达。结果:网络药理学及转录组学测序分析表明,虎黄洗剂潜在靶点与炎症调控密切相关。创面局部应用虎黄洗剂可促进糖尿病创面愈合,苏木精-伊红(hematoxylin and eosin,HE)染色结果显示虎黄洗剂可促进糖尿病创面上皮化,ELISA检测结果提示虎黄洗剂可抑制创面组织炎症因子IL-6、TNF-α及IL-1β表达。结论:虎黄洗剂可抑制糖尿病创面炎症反应,促进创面愈合。
文摘This paper reports that four new glycosyl esters of Vanillic Acid were synthesized by the reaction of vanillic acid with O-acetylglucopyranosyl bromide or O-acetylgalacopyranosyl bromide,O-acetylxylopyranosyl bromide and O-acetyllactosyl bromide in the presence of Ag2O respectively;and vanillic acid-4-β-D-2’,3’,4’,6’-acetyl glucoside-β-D-2",3",4",6"-acetyl glucoester by vanillic acid with O-acetylglucopyranosyl bromide in the presence of Bu4NBr.Their structures were confirmed by IR,1H NMR,13C NMR,HMBC and MS.And spectra data proved that the products are of β-configuration.
