Profilin has recently been identified as an actin-binding protein in higher plants. A cDNA clone (designated Repro) encoding profilin gene was isolated from rapeseed ( Brassica napus L. cv. canadian Tween) using RT-PC...Profilin has recently been identified as an actin-binding protein in higher plants. A cDNA clone (designated Repro) encoding profilin gene was isolated from rapeseed ( Brassica napus L. cv. canadian Tween) using RT-PCR technique. Sequence analysis showed 82% similarity to Zea mays L. ZmPro3, 85% to Arabidopsis AthPRF1, 82% to Nicotiana tabacum L. NTPRO, 81% to Oryza sativa L. profilin A. A new full-length cDNA was obtained by 5'-RACE and 3'-RACE techniques. Sequence analysis showed that the size of full-length cDNA is 672 bp which contains a major open reading frame of 134 amino, acids, 5' and 3' untranslated regions and a long Poly (A) tail. Northern blot analysis showed that the profilin gene is a pollen and anther specific gene.展开更多
In this study, testtube plantlets of O.Violaceus were cultured on MS media and treated with low temperature, phytohormones, etc. The following results were obtained: 1. The cold treatment of 5～7℃ for more than 7 da...In this study, testtube plantlets of O.Violaceus were cultured on MS media and treated with low temperature, phytohormones, etc. The following results were obtained: 1. The cold treatment of 5～7℃ for more than 7 days was needed for flower initiation of the testtube plantlets originated from germinated seeds. 2. When cultured on the MS media supplemented with 2mg/L gibberellin (GA3), the testtube plantlets could initiate flowers without cold treatment. 3. When the MS media was supplemented with a reduced amount of NH4NO3, flower initiation of the testtube plantlets could be accelerated. The results of the study on flower development of O.Violaceus may be useful for the production of testtube flowers of this ornamental plant species.展开更多
文摘Profilin has recently been identified as an actin-binding protein in higher plants. A cDNA clone (designated Repro) encoding profilin gene was isolated from rapeseed ( Brassica napus L. cv. canadian Tween) using RT-PCR technique. Sequence analysis showed 82% similarity to Zea mays L. ZmPro3, 85% to Arabidopsis AthPRF1, 82% to Nicotiana tabacum L. NTPRO, 81% to Oryza sativa L. profilin A. A new full-length cDNA was obtained by 5'-RACE and 3'-RACE techniques. Sequence analysis showed that the size of full-length cDNA is 672 bp which contains a major open reading frame of 134 amino, acids, 5' and 3' untranslated regions and a long Poly (A) tail. Northern blot analysis showed that the profilin gene is a pollen and anther specific gene.
文摘In this study, testtube plantlets of O.Violaceus were cultured on MS media and treated with low temperature, phytohormones, etc. The following results were obtained: 1. The cold treatment of 5～7℃ for more than 7 days was needed for flower initiation of the testtube plantlets originated from germinated seeds. 2. When cultured on the MS media supplemented with 2mg/L gibberellin (GA3), the testtube plantlets could initiate flowers without cold treatment. 3. When the MS media was supplemented with a reduced amount of NH4NO3, flower initiation of the testtube plantlets could be accelerated. The results of the study on flower development of O.Violaceus may be useful for the production of testtube flowers of this ornamental plant species.
