摘要
目的:运用肝肾同源理论及网络药理学研究肉苁蓉–巴戟天干预迟发性性腺功能减退症可能存在的作用机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)对肉苁蓉–巴戟天药对中活性成分靶点进行筛选,通过GeneCards、OMIM数据库检索迟发性性腺功能减退症靶点,取得交集基因后使用Cytoscape3.9.0建立化合物–疾病–靶点调控网络,基于生物学信息注释数据库(DAVID)对核心靶点数据信息进行下载,并用R软件绘制气泡图取得(GO)功能富集分析与基因组百科全书(KEGG)通路富集分析。结果:得到肉苁蓉–巴戟天药对的22个活性成分和271个潜在靶点,相关的疾病靶点2462个。GO富集分析主要包括正向调节凋亡过程、细胞因子介导的信号通路、药物反应、线粒体、蛋白质结合、酶结合等功能途径。KEGG通路分析主要包括丝裂原活化蛋白激酶(MAPK)信号通路、肿瘤坏死因子(TNF)信号通路、缺氧诱导因子1 (HIF-1)信号通路、糖尿病并发症中的RAGE信号通路等。结论:基于网络药理学及肝肾同源理论初步揭示出肉苁蓉–巴戟天药对干预LOH具有活性成分及作用靶点多样、作用途径广泛的特点,其作用机制可能与有效成分槲皮素、β谷甾醇,核心靶点及相关通路对睾丸间质细胞及支持细胞的调节,影响睾酮分泌有关。
Objective: Study the possible mechanism of Cistanches Herba and Morindae Officinalis Radix inter-vening late-onset hypogonadism (LOH) by using the theory of “homogeny of liver and kidney” and network pharmacology. Methods: The active component targets of Cistanches Herba and Morindae Officinalis Radix were screened through Traditional Chinese Medicine Systems Pharmacology Da-tabase and Analysis Platform (TCMSP). The targets of late-onset hypogonadism were searched through Gene Cards and OMIM databases. After obtaining the intersection genes, the com-pound-disease-target regulation network was established by using Cytoscape 3.9.0, and the core target data information was downloaded based on the database for annotation, visualization and integrated discovery (DAVID). The bubble diagram was drawn with R software to obtain (GO) func-tional enrichment analysis and genomic encyclopedia (KEGG) pathway enrichment analysis. Results: 22 active components and 271 potential targets of Cistanches Herba-Morindae Officinalis Radixwere were obtained, and 2462 related disease targets were obtained. GO enrichment analysis mainly in-cludes functional pathways such as positive regulation of protein phosphorylation, cyto-kine-mediated signaling pathway, angiogenesis, mitochondrion, zincion binding, transcription fac-tor binding and so on. KEGG pathway analysis mainly includes MAPK signaling pathway, TNF sig-naling pathway, HIF-1 signaling pathway, and RAGE signaling pathway in diabetic complications. Conclusion: Based on the network pharmacology and the theory of “homogeny of liver and kidney”, it is preliminarily revealed that Cistanches Herba and Morindae Officinalis Radix have the charac-teristics of diverse active components, action targets and wide action pathways in the intervention of LOH, and its action mechanism may be related to the effective components quercetin, β Sitosterol, core targets and related pathways regulating Leydig cells and Sertoli cells, affecting testosterone secretion.
出处
《临床医学进展》
2022年第8期7449-7461,共13页
Advances in Clinical Medicine
