摘要
目的 探讨干扰素及拉米夫定 (3TC)的体外抗乙型肝炎病毒作用。方法 以HepG2 2 ,2 ,15为细胞模型 ,实验共观察 9d ,于第 3、6、9天收集培养上清进行HBsAg定量检测 ;收集细胞提取总RNA ,行RT PCR及荧光实时定量PCR测细胞内乙肝病毒mRNA水平。结果 实验第 3天及第 6天 ,对照组和各实验组之间HBsAg滴度及病毒mRNA水平差异无显著性 ;实验第 9天 ,干扰素及拉米夫定组HBsAg和病毒mRNA水平均较对照组明显降低 ,α 干扰素浓度 5 0 0IU/ml时抑制作用达最大 ,3TC浓度为 5 μg/ml时抑制作用达最大。 结论 干扰素及拉米夫定均可抑制HepG2 2 ,2 ,15 细胞内乙肝病毒基因的转录和表达 ,提示两种药物都可直接抑制乙肝病毒复制。
Objective To evaluate the in vitro inhibitive activities of interferon-alpha and lamivudine against hepatitis B virus. Methods HepG2 2,2,15 cell were used as cell model. At 3rd, 6th and 9th day, supernatant was collected for HBsAg quantitative assay, total RNA was isolated for RT-PCR and realtime-PCR assay. Results No significant difference were found among all groups about HBsAg titer and virus mRNA level at 3rd and 6th day. At 9th day, HBsAg titer and virus mRNA level from interferon groups and lamivudine groups were significant lower than those of control. It showed that they could get maximal inhibition effect on concentration of 500 IU/ml (interferon) and 5 μg/ml (lamivudine). Conclusions Interferon and lamivudine can inhabit transcription and expression of HBV gene in HepG2 2.2.15 cell. It indicated that interferon and lamivudine can inhibit HBV replication directly.
出处
《疾病控制杂志》
2004年第3期209-212,共4页
Chinese Journal of Disease Control and Prevention
