摘要
目的 探讨血清沙眼衣原体 (CT)及其热休克蛋白 6 0 (C HSP6 0 )抗体反应与异位妊娠 (EP)的关系。方法 检测了4 7例EP患者 (EP组 )和 4 6例宫内妊娠妇女 (对照组 )血清CT抗体。用基因工程的方法重组C HSP6 0 ,以纯化后的C HSP6 0蛋白为抗原 ,采用酶联免疫吸附法 ,检测了两组妇女血清C HSP6 0抗体水平。结果 EP组和对照组血清沙眼衣原体IgG抗体阳性率分别为 6 8.1 %和 34.8% ,两组有显著性差异 (P <0 .0 5 ) ;EP妇女C HSP6 0IgG抗体阳性率为 2 5 .5 % ,高于对照组阳性率4 .3% ,两者有显著性差异 (P <0 .0 5 )。在沙眼衣原体血清学阳性妇女中 ,EP组C HSP6 0IgG抗体阳性率为 34.4 % ,与宫内妊娠组 0 % (0 /1 6 )相比 ,有显著性差异 (P <0 .0 5 )。两组在血清沙眼衣原体IgM、C hsp6 0IgM抗体水平上皆无显著性差异 (P >0 .0 5 )。结论 女性生殖道沙眼衣原体感染是导致EP的重要原因 ,C HSP6
Objective To investigate the prevalence of antibody to chlamydia trachomatis and chlamydial heat shock protein 60(C HSP60) in women with ectopic pregnancy(EP).Methods Fourty seven women with ectopic pregnancy (EP group),46 women with intrauterine pregnancy (control group) were investigated as a case control study.An ELISA using purified recombinant C HSP60 was developed to detect antidobies to C.trachomatis and C HSP60 in serologic samples.Results IgG antibody to C.trachomatis was present in 32(68.1%) of 47 women with ectopic pregnancy compared with 16(34.8%) of 46 women with intrauterine pregnancy ( P <0.05);C HSP60 IgG positive rate was signficantly higher in EP group than in the control group(25.5% versus 4.3 %, P < 0.05 ).Among the C.trachomatis seropositive women,antibody to C HSP60 was present in 11(34.4%) of women with ectopic pregnancy compared with 0(0%) of 16 with introurine pregnancy ( P <0.05).There were no signficant differences in the positive rate of C.trachomatis IgM and C hsp IgM between two groups.Conclusions There is a strong association between antibody response to C HSP60 and the development of C.trachomatis associated ectopic pregnancy.So C HSP60 plays an important role in the pathogenesis of ectopic pregnancy.
出处
《重庆医学》
CAS
CSCD
2004年第6期853-855,共3页
Chongqing medicine
关键词
异位妊娠
热休克蛋白60
沙眼衣原体
ectopic pregancy
heat shock protein 60
chlamydia trachomatis
