摘要
目的 :观察肝心康对实验性肝纤维化的作用。方法 :用 40 %四氯化碳复合因素皮下注射致大鼠肝纤维化模型 ,肝心康治疗结束 ,断头取血分离血清 ,检测肝功能、血清透明质酸 (HA)、层粘连蛋白 ;分离肝组织 ,用免疫组化方法检测肝组织内转换生长因子 β1(TGF β1)的表达。 结果 :肝心康能明显降低肝纤维化大鼠的转氨酶 ,增加肝脏对白蛋白的合成 ,降低肝纤维化血清学指标 ,抑制TGF β1的表达。 结论 :肝心康具有保肝及抗肝纤维化作用 ,其作用机理可能和抑制TGF
Objective: To study the effect of GXK on the liver fibrosis in rats induced by intracutaneous injection of carbon tetrachloride(CCl 4). Methods: The rats liver fibrosis model was made by intracutaneous injection of CCl 4 twice a week in rats for 6 weeks. From the 1st day after the CCl 4 injection, GXK, at the dose 10g/kg or 2g/kg or 0.4g/kg were given once a day for 42 days. At the end of the experiment, the animals were sacrifice by cutting neck. The serum and liver were collected for measuring these liver function,serumhyaluronic acid(HA), laminin(LM) and the expression of TGF β1 by immunohistochemical technique. Results: The levels alamine trasaminase(ALT),aspartate trasaminase(AST) were markedly lessened as compared with those of the model group.Albumin(ALB) was increased. The levels of serum fibrosis markers HA ,LM were lower in GXK treatment group than in model group. On the immunohistochemical results,the positive stain of TGF β1 were stronger in model group than in GXK treatment group. Conclusions: GXK improved the liver function,decreased the serum hepatic fibrosis markers and inhibited the expression of TGF β1 in liver fibrosis rats.
出处
《中药药理与临床》
CAS
CSCD
2004年第2期34-36,共3页
Pharmacology and Clinics of Chinese Materia Medica
