摘要
目的 观察低频超声对人卵巢癌COC1体外生长的影响。方法 以低频超声 (频率 0 .2 4MHz,剂量 5 .12W /cm2 × 5s)辐照COC1细胞 ,采用MTT观察超声对COC1细胞增殖的抑制作用 ;流式细胞仪(FCM)、TUNEL法和光、电镜检测细胞凋亡。结果 超声波能够显著抑制卵巢癌COC1细胞增殖 ,辐照后 12h、2 4h、36h和 4 8h ,其MTT光吸收值与对照组相比显著降低 ;FCM检测超声辐照后 6h的COC1细胞凋亡率达 (2 5 .5 7± 4 .6 8) % ;同时TUNEL法观察到棕褐色着染的凋亡细胞 ;相差显微镜及电镜下可见凋亡小体形成等典型形态学改变。结论 低频超声辐照对人卵巢癌COC1细胞体外生长具有明显的抑制作用 ,并可诱导其凋亡。
Objective To investigate the effect of Low-Frequency Ultrasound on proliferation and apoptosis of human ovarian cell line COC1.Methods The growth inhibitory effect of Ultrasound Exposure( 0.24 MHz, 5.12 W /cm 2× 5 s ) on COC1 cell was observed by MTT colormetric assay;Cell apoptosis was analyzed using flow cytometry(FCM);Terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling(TUNEL);Phase contrast light microscope and Electron microscope were applied to detect the morphological changes of COC1 cell. Results Sonication could obviously inhibit the proliferation of COC1 cell;The absorbance value(A_ 570 )of MTT significantly decreased at 12h,24h,36h and 48h after sonication as compared to control group;The apoptosis rate was found to reach ( 25.57 ± 4.68 )% by FCM,and brown-colored positive apoptotic cells were observed with TUNEL method at 6h after sonication;Typical morphological changes of apoptosis such as apoptotic body were found under Phase contrast light microscope and Transmission electron microscope.Conclusion The results suggest Low-Frequency Ultrasound can suppress proliferation and induce apoptosis in vitro in human ovarian cell line COC1.
出处
《肿瘤防治研究》
CAS
CSCD
2004年第5期249-251,F002,共4页
Cancer Research on Prevention and Treatment
基金
国家自然科学基金资助项目 (30 2 0 0 0 6 0 )
