摘要
目的 分析乙型肝炎病毒(HBV)引起的肝炎肝硬化患者外周血Ⅱ型树突状细胞(pDC 2)的数量和产生α干扰素的功能,并分析其与患者淋巴细胞亚群和发生机会性感染的关系。 方法 采用流式细胞分析技术对27例HBV引起的肝炎肝硬化患者进行研究,对患者外周血p DCN和淋巴细胞亚群进行检测;用体外灭活的1型单纯疱疹病毒(HSV—1)刺激并培养外周血单个核细胞(PBMCs),检测培养上清液中。干扰素的产量。 结果 肝炎肝硬化患者pDC2的比例、细胞数和产生α干扰素的功能均降低;pDC2的数量与CD_8^+ T细胞及NK细胞数量高低存在正相关,而且发生机会性感染组患者的pDC2、CD_8^+ T细胞及NK细胞数均低于未感染组。 结论 肝炎肝硬化患者外周血pDC2数量和功能下降,伴随CD_8^+ T细胞和NK细胞数平行降低,与肝炎肝硬化疾病进程和机会性感染有关。
Objective To identify the frequency and interferon(IFN)-alpha-producing ability of circulating type 2 pre-dendritic cells(pDC2) and evaluate its role in liver cirrhotic patients with chronic HBV infection. Methods 27 liver cirrhotic patients were included in our study and 25 patients with chronic hepatitis B and 25 healthy individuals were enrolled as controls. The numbers of circulating pDC2 and lymphocytes including CD_4^+ T cells, CD_8^+ T cells, NK cells as well as B cells were analyzed by flow cytometry. The IFN-alpha-producing function of peripheral blood mononuclear cells (PBMCs) representing the circulating pDC2 was determined by ELISA assay after stimulated by ultraviolet-inactivated herpes simplex virus-1(UV-HSV-1). Results The number of pDC2 were(7.21±2.38)×10~6/L, (4.49±3.08)×10~6/L and(2.89±1.17)×10~6/L for healthy control, chronic hepatitis B and cirrhotic patients respectively. Both the number and IFN-alpha-producing function of circulating pDC2 in liver cirrhotic patients significantly lower than that in healthy subjects. There was a correlated simultaneous decrease numbers of circulating CD_8^+ T cells, NK cells in HBV-infected cirrhotic patients. Furthermore, cirrhotic patients with opportunistic infections have lower numbers of pDC2, CD_8^+ T cells and NK cells compared to those without opportunistic infections. Conclusions Liver cirrhotic patients with chronic HBV infection have a significant decrease of circulating pDC2 level and IFN-alpha-producing function. The decreased number and function of pDC2, together with the lower number of CD_8^+ T cells and NK cells may result in the decline of host immune response, which may partially contribute to the disease progression of HBV infection and opportunistic infections.
出处
《中华肝脏病杂志》
CAS
CSCD
2004年第5期274-277,共4页
Chinese Journal of Hepatology
基金
国家自然科学基金(30271230)
