摘要
为探讨单链抗体 (ScFv )对重症肌无力 (MG )的特异性免疫治疗作用 ,从分离自MG患者胸腺的抗乙酰胆碱受体(AchR )抗原结合片段Fab6 37构建单链抗体ScFv6 37。应用放射免疫测定法测定了ScFv6 37与刺激抗原人AchR的特异性结合活性以及与相关抗原大鼠AchR和电鳐AchR的交叉反应 ,应用竞争性ELISA测定抑制致病性抗AchR完整抗体IgG6 37与人AchR结合活性。结果发现ScFv6 37只能与人AchR结合 ,不能与大鼠和电鳐的AchR结合。对IgG6 37与人AchR结合的抑制率为 17 5 %～ 32 9%。表明单链抗体对AchR具有一定的“保护”作用。
To investigate the specific suppressive effect of the antibody against the single chain variable fragment (ScFv) on myathenia gravis (MG), ScFv637 was constructed from the antigen-binding fragment 637 (Fab637) of anti-acetylcholine receptor (AchR) antibody derived from thymus of a patient suffered from MG. The specific binding activity of ScFv637 with the human AchR of stimulating antigen (hAchR) and the cross-reactions with the rat AchR of related antigens (rAchR) and torpede AchR (tAchR) were determined by radioimmunoassay (RTA). The suppressive effect of ScFv637 on the binding activity of the pathogenic intact antibody against AchR was measured by competitive ELISA. It was found that ScFv6378 could bind with hAChR, but was unable to bind with rAchR or tAchR. Furthermore, it was also capable to inhibit the binding with the pathogenic anti AchR antibody IgG637 against hAchR by 17.5%～32.9%, indicating that ScFv637 has a protective effect on hAchR, which holds promise for the immunotherapy of ScFv in patients with MG.
出处
《现代免疫学》
CAS
CSCD
北大核心
2004年第3期235-237,244,共4页
Current Immunology
基金
国家自然科学基金资助项目 ( 3 9960 0 72 )
关键词
重症肌无力
单链抗体
乙酰胆碱受体
myasthenia gravis
single chain variable fragment
acetylcholine receptors
