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转高粱C4型NADP-ME基因水稻植株的光合生理特征 被引量:4

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摘要 NADP-苹果酸酶(NADP-ME)是C4型植物C4光合途径的一个关键酶。利用RT-PCR结合筛选cDNA文库技术,分离得到了编码高粱(Sorghum vulgareL.)C4型NADP-ME的全长cDNA。该cDNA全长为2139bp,其开放可读框为1911bp,共编码636个氨基酸和一个终止密码子(GenBank登求号为AY274836)。利用农杆菌介导的转化系统将其转入水稻品种“农垦58”。经Southern杂交、Northern杂交和酶活性检测表明,高粱C4型NADP-ME可以在水稻中有效表达,酶活性可被提高1~7倍。对转基因水稻进行光合生理检测表明,转NADP-ME基因水稻CO2交换特征没有明显改变,但是在中午强光条件下光抑制加剧。
出处 《Acta Botanica Sinica》 CSCD 2004年第7期873-882,共10页 Acta Botanica Sinica(植物学报:英文版)
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  • 1王强,郝迺斌,白克智,卢从明,戈巧英,匡廷云,张其德,董凤琴.Characterization of photosynthesis,photoinhibition and the activities of C_4 pathway enzymes in a superhigh-yield rice,Liangyoupeijiu[J].Science China(Life Sciences),2002,45(5):468-476. 被引量:9
  • 2Seiichi Toki.Rapid and efficientAgrobacterium-mediated transformation in rice[J].Plant Molecular Biology Reporter.1997(1)
  • 3Lo?c Lepiniec,Eliane Keryer,Herve Philippe,Pierre Gadal,Claude Crétin.Sorghum phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution[J].Plant Molecular Biology.1993(3)
  • 4Lo?c Lepiniec,Eliane Keryer,Denis Tagu,Pierre Gadal,Claude Crétin.Complete nucleotide sequence of a sorghum gene coding for the phosphoenolpyruvate carboxylase involved in C4 photosynthesis[J].Plant Molecular Biology.1992(2)
  • 5Qiu G X.The efficiency of plant photosynthesis[].Plant Physiology and Molecular Biology.1992
  • 6T oki,S.RapidandefficientAgrobacter ium-mediatedtra ns forma-tioninrice,PlantMol.Biol[].Reporter.1997
  • 7Ku,M.S.B,Agarie,S,Nomura, M. etal.High-lev elexpressi on ofmai zephosphoenolpyruva tecarboxyla se intransgen icri ceplants[].Nature Biotechnology.1999
  • 8Fukayama,H,Tsuchida,H,Aga ri e,S.etal.Signi ficantaccumu- lationofC (4)-sp ecificpyr uvate orthopho sphatedi ki nasei naC (3) plant,rice[].Plant and Physiology.2001
  • 9Lepiniec,L,Keryer,E,T agu,D.etal.Completenu cleotides e- q uenceof asor ghumge nec odi ngforthephosphoenol pyruvatecar boxylase invo lvedinC4photosynthesis[].Plant Molecular Biology.1992
  • 10Hiei,Y,Ohta,S,Ku ma shiro,T.etal.Efficienttran sf ormationof rice (Oryz asativaL .)mediatedbyAgr oba cteriuma ndsequen ce ana lysisofthe bo undariesoftheT-DNA[].ThePlantJ.1994

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