摘要
将藏猪外周血淋巴细胞在伴刀豆球蛋白 A(Con A)的刺激下体外培养 70 h后 ,提取激活淋巴细胞总 RNA,应用RT- PCR技术扩增出藏猪淋巴细胞白细胞介素 - 2 c DNA (TPIL - 2 ) ,克隆到 p MD- T载体上并测序。测序结果显示 ,克隆的TPIL- 2 c DNA全长为 5 0 3个碱基 ,开放阅读框 (ORF)为 4 6 5个碱基 ,编码 15 4个氨基酸 ,分子量为 17.4 kd,等电点为 5 .38;疏水氨基酸 4 0 .3% ,亲水氨基酸 37.0 % ,碱性氨基酸 11% ,酸性氨基酸 11.7%。此 c DNA与报道猪的 IL - 2同源性为 10 0 % ,证实克隆到了藏猪白细胞介素 - 2基因 c DNA。
The interleukin-2 cDNA of Tibet Pig was amplified by RT-PCR from total RNA extracted from blood lymphocytes,which were cultivated and stimulated with 10 μg/ml ConA in vitro for 70 hours.Then the amplified cDNA was cloned into pMD-T vector and named as TPIL-2.The nucletide acid sequence of cloned Tibet pig interleukin-2 cDNA was determined.The length of the TPIL-2 was 503 bp(ORF was 465 bp),encoding a peptide of 154 amino acids whose molecular is 17.4 kd and pI is 5.38.By blasting the homologous sequences in GenBank databases,the sequence of Tibet pig interleukin-2 gene from lymphocyte is identical to the interleukin-2 genes previously cloned from spleen cell and fibroblast cell of other porcines.
出处
《中国兽医杂志》
CAS
北大核心
2004年第6期3-5,共3页
Chinese Journal of Veterinary Medicine
基金
国家自然科学基金课题 (3 0 170 70 3 )资助
