摘要
采用群体和单体饲养的方法,用转双抗虫基因741杨叶片饲养舞毒蛾和美国白蛾幼虫,测定其死亡率、各龄期的发育速率、体重增加速率和取食效率。结果表明:4个转双抗虫基因的741杨株系对舞毒蛾和美国白蛾均有明显抗虫效果,主要表现为对低龄幼虫的毒杀作用和对高龄幼虫生长发育的抑制作用。用转抗虫基因杨树叶片饲养的昆虫幼虫死亡率均高于对照,最高可达100%,而且,可导致存活幼虫的不正常的发育,使其发育历期明显延迟,发育速率、体重增加速率和取食增长率均显著降低。
The larvae of Gypsy moth (Lmantria dispar L.) and American white moth (Hyphantria cunea Drury) were fed on transgenic hybrid poplar with individual and group feeding method. The mortality, development speed,weight increasing rate and feeding efficiency of each instar of the insects were determined. The results showed that four transgenic hybrid poplar clones all had insect-resistant effect on Gypsy moth and American white moth, which mainly indicated the killing effect on lower instar larvae and inhibition from development on higher instar larvae. The mortality of larvae fed on the leaves of transgenic hybrid poplar was higher than that of larvae fed on the leaves of control, and the highest was up to 100%. Moreover, transgenic hybrid poplar can bring about the surviving insect larvae abnormal development, their development period was postponed obviously, and the development speed, weight increasing rate and feeding increasing rate of them were all greatly decreased.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2004年第3期60-63,共4页
Journal of Hebei Agricultural University
基金
国家自然科学基金资助项目(30070626)
河北省自然科学基金资助项目(302312)
关键词
抗虫基因
741杨
抗虫性
insect-resistant genes
hybrid poplar 741
insect resistance
