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高产优质抗病棉花品种中棉所12号细菌人工染色体(BAC)文库构建 被引量：3

BAC library construction of Zhongmiansuo 12 with high yield, high quality and disease resistance
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摘要 中棉所12号是我国自育的高产、优质、抗枯萎病、耐黄萎病棉花品种,其重要创新是使抗性和产量、品质得到结合改良和提高。本研究以plndigoBAC-5为载体,对中棉所12号进行了细菌人工染色体(BAC)文库构建。初步建立的文库含有38800个克隆,覆盖2倍基因组。对文库中132个重组克隆的分析表明:插入片段为50～150kb,平均大小为120kb;大于100kb的克隆占87 7%,大于110kb的克隆占56%;空载率小于1%。该文库的构建为深入开展棉花基因组有关研究奠定了基础。 The bacterial artificial chromosome(BAC) library of Zhongmiansuo 12, a Chinese elite cotton variety with high yield, high quality and disease resistance, was constructed based on plndigoBAC-5 vector. The library included 38 800 clones and was two haploid genome equivalents based on a AD genome size of 2 240 Mb. Analysis results of 132 recombinants indicated that the insert DNA size ranged from 50 to 150 kb, averaged 120 kb. As much as 87.7% clones had inserts over 100 kb, and 56% clones over 110 kb. The empty clone was less than 1% in the library. This library provided a useful tool for the cotton genome research.
作者 郑拥民 王省芬 张桂寅 李喜焕 马峙英
机构地区 河北农业大学农学院河北省作物种质资源重点实验室
出处 《河北农业大学学报》 CAS CSCD 北大核心 2004年第3期17-20,共4页 Journal of Hebei Agricultural University
基金 国家自然科学基金(30270848) 教育部重点科技项目(204017)
关键词 棉花 中棉所12号 细菌人工染色体 文库构建 cotton Zhongmiansuo 12 bacterial artificial chromosome (BAC) construction of library
分类号 S512 [农业科学—作物学]
  • 相关文献

参考文献21

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二级参考文献40

共引文献253

同被引文献26

  • 1Jian-Mei Yin Wang-Zhen Guo Tian-Zhen Zhang.Construction and Identification of Bacterial Artificial Chromosome Library for 0-613-2R in Upland Cotton[J].Journal of Integrative Plant Biology,2006,48(2):219-222. 被引量：4
  • 2王省芬,马骏,马峙英,张桂寅,郑拥民.高纤维强力棉花种质系苏远7235 BAC文库的构建[J].棉花学报,2006,18(4):200-203. 被引量：10
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引证文献3

二级引证文献10

河北农业大学学报
2004年 第3期

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