摘要
应用基因测序法对我国12个省24份脊髓灰质炎1型病毒标本散了VP1/2A片段的测序工作,并与疫苗株sabin 1型参考株的序列,我国台湾省和其他国家15株病毒的序列做了比较。由计算机PCGene程序分析处理,构建出两株关系树,初步揭示了我国大陆6个基因型病毒之间,以及它们与台湾省和其他国家病毒之间的分子流行病学关系。
24 strains of poliovirus type l isolated in 12 different provinces in China were sequenced in the VP1/2A region(l50 nucleiotides)by the dide-oxy chain termination method. After comparing these sequences with the Sabin 1 reference strain and with 15 strains from province Taiwan and other neighbouring countries using the gene alignment program from the PCGene, 2 dendrograms were constructed. We found 6 independent geno types among the Chinese viruses and 14 genotypes among the world viru ses. This interesting finding strongly suggested that there were molecular epidemiological relationships among viruses from China and other countries.
出处
《病毒学报》
CAS
CSCD
北大核心
1993年第4期337-344,共8页
Chinese Journal of Virology
关键词
脊髓灰质炎
Ⅰ型病毒
分子流行病学
Poliovirus type 1, Dideoxy chain termination method, VP1/2A region, Program PCGene, Molecular epidemiology
