摘要
目的 :研究DOC - 2对卵巢癌细胞TC - 1的影响。方法 :用脂质体介导的转染技术 ,将含有全长DOC - 2cDNA的真核表达重组质粒和空载体质粒 ,分别导入不表达DOC - 2的TC - 1细胞 ,观察细胞增殖能力的改变。结果 :共获得 10个DOC - 2转染克隆 (TC -p93)和 6个空载体克隆 (TC -pcDNA3.1) ;转染后细胞形态与TC - 1细胞无显著差别 ;TC -p93细胞生长明显比TC-pcDNA3.1和TC - 1细胞慢 ;TC -p93细胞形成集落数显著少于TC - 1与TC -pcDNA3.1细胞 ;TC -p93G1期细胞百分比高于TC - 1和TC -pcDNA3.1细胞。结论 :DOC - 2基因可明显抑制癌细胞生长 ,可作为卵巢癌基因治疗的目的基因之一。
Objective:To study the effects of DOC-2 on proliferation of human ovarian cancer cell line TC-1 . Methods: Recombinant eukaryotic expression vector pcDNA3.1-P93 containing exogenous human DOC-2cDNA and vector with neomycin resistance gene only were introduced by lipofectamine-mediated gene transfection into TC-1 cell line which does not express DOC-2 endogenously. The clones obtained were detected for their efficiency of transfection and effects of vector expression and observed for their proliferous ability.Results: Ten clones named TC-p93 and 6 clones named TC-pcDNA3.1 were obtained . The morphology of cells either from TC-p93 and TC-pcDNA3.1 did not change significantly with respect to their parental TC-1;The growth rate of TC-p93 cells was inhibited,while the cell growth curve of TC-pcDNA3.1 was similar to that of TC-1.The number of colones formed by TC-p93 was significantly less than that formed by TC-1 or by TC-pcDNA3.1; The percentage of phase G 1 cells increased and that of phase S cells decreased by analysing cell cycle. Conclusion: [WT5BZ]DOC-2 gene plays an important role in generation and development of ovarian carcinoma. This study might provide the experimental evidence for the gene therapy in human ovarian cancer.
出处
《西北国防医学杂志》
CAS
2004年第3期207-209,共3页
Medical Journal of National Defending Forces in Northwest China
