摘要
目的 建立良好的人类蜕膜细胞培养方法。方法 选用早孕人工流产蜕膜组织进行体外培养 ,采用胰蛋白酶和EDTA消化 ,进行全组分蜕膜细胞培养 ,利用传 3代的方法对蜕膜基质细胞进行分离提纯。制作细胞爬片 ,用泌乳素 (prolactin ,PRL)免疫组化试剂盒检测培养细胞成分。结果 免疫组化显示体外培养传代后 97%蜕膜细胞为蜕膜基质细胞。结论 实验方法经济实用、简单 ,简化了蜕膜细胞的提纯程序 ,获得的蜕膜基质细胞纯度高。
Objective To develop a simple and satisfactory method to culture human decidual stromal cells (DSC). Methods Abortion decidual tissues were digested with trypsin and EDTA. All components of the decidual cells were cultured together. After being transferred for three times, the DSC were isolated and purified, and finally identified by immunohischemistry with prolactin (PRL). Results The immunohischemistry revealed that the PRL positivity was 97%. Conclusion Compared with the conventional DSC extraction procedure, the present method is simple, economical and practical, in which the isolation procedures are simplified and the product is of high purity.
出处
《徐州医学院学报》
CAS
2004年第3期235-238,共4页
Acta Academiae Medicinae Xuzhou
基金
卫生部科研基金资助项目 (92 2 4 1)
