摘要
介绍一种用麦胚凝集素(WGA)沉淀法测定血清中骨型碱性磷酸酶(AKP)的方法。该法简单、重复性好,批内及批间CV为2.4%和7.98%,骨AKP测定值与计算值的相关性好(r=0.995)。测定131例不同年龄、性别正常成人骨AKP,建立了成人骨AKP参考值范围,它们与性别、年龄有关。测定部分代谢性骨病及恶性肿瘤患者,证明本法适用于临床。
WGA(Triticum Vulgaris) was weed to precipitate bone alkaline phosphutas(AKP) in serum. The method showed simple and reliable and the intra—and inter—assay CV were 2.4% and 7.9% respectively. The reference ranges of bone AKP from normal adults wore established and the levels were related to the sex and age. The AKP level of serum in patients with hyperparathyroidism, Paget's desease and bone metastases increased significantly. The results indicate that this method is a useful tool for studing metabolic bone desases.
出处
《北京医学》
CAS
北大核心
1993年第6期337-339,共3页
Beijing Medical Journal
