摘要
应用杂交瘤技术制备了蛋白激酶C(PKC)的单克隆抗体,用蛋白A-Sepharose-CL4B协同沉淀复合物分析单抗识别的蛋白,分子质量与PKC相同。采用该抗体对正常和转化的C_3H_(10) T_(1/2)细胞进行免疫荧光观察,发现它们的PKC含量明显不同。但荧光分布都主要集中在细胞质和细胞膜部分。
Hybrid clones are selected with HAT medium and tested for anti-PKC antibody by ELISA coating with purified protein kinase C(PKC). After the positive clones are subcloned by limited dilute method they were injected into pristane-primed Balb/C mice to prepare the ascites fluid. The enzyme is distributed in the plasma membrane and cytoplasm with immu- nocytochemical method using the prepared antibody and differences between C_3H_(10) T_(1/2) cell and transformed C_3H_(10) T_(1/2) cell are observed. SDS-PAGE anal- ysis of the immunoprecipitates of crude extract of C_3H_(10)T_(1/2) cell with the monoclone antibody desolved a protein with the same molecular weight of protein kinase C.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
1993年第1期137-141,共5页
Journal of Beijing Normal University(Natural Science)
基金
国家自然科学基金
关键词
蛋白激酶C
单克隆抗体
杂交瘤
protein kinase C, monoclone antibody, hybridroma, cell fusion, immunocytochemistry
