摘要
使用荧光比率方法研究血管平滑肌细胞钙动力学变化在重症休克血管反应性降低中的作用.复制SD大鼠失血性休克模型,分离肠系膜细动脉血管平滑肌(ASMC),使用荧光探针Fluo-3/AM、FuraRed双标记比率方法结合激光扫描共聚焦显微成像技术测定单个平滑肌细胞钙动力学变化,观察ATP敏感钾通道(KATP)特异性阻滞剂优降糖对血管反应性和钙动力学影响.实验结果发现休克后2h(失代偿期),血管反应性明显降低,norepinephrine作用带来的平滑肌细胞内钙离子浓度升高的程度明显减弱;加入优降糖可明显提高NE对平滑肌细胞内钙离子的升高作用,改善细动脉对NE的反应性,带来血管反应性部分恢复.
Using a ratio fluorescent approach to study the role of the kinetic changes of calcium in vascular hyporeactivity of severe hemorrhagic shock.The hemorrhagic shock was made and mesenteric arteriolar smooth muscle cells (ASMC) were isolated.The calcium kinetic changes were measured with two highly sensitive Ca2+fluorescent labeling in conjunction with Laser Scanning Confocal Microscope (LSCM).The effect of glibenclamide, a specific inhibitor for ATP sensitive potassium (KATP) channel, on vascular reactivity and the concentration of intracellular calcium ions were studied.The results showed that hemorrhagic shock for 2 hours caused a obvious reduction of vascular reactivity and i rising on the effect of norepinephrine were reduced.Glibenclamide could improve NE effect on i in ASMCs.Glibenclamide, a specific inhibitor for ATP sensitive potassium (KATP) channel can increase the intracellular i and improve the vascular reactivity in severe hemorrhagic shock.
出处
《生命科学研究》
CAS
CSCD
2004年第2期154-158,共5页
Life Science Research
基金
国家自然科学基金资助项目(30070368)
国家973课题项目(G1999054308)
深圳市科技攻关项目
关键词
共聚焦显微术
荧光比率
血管反应性
钙离子
laser scanning confocal microscope
ratio fluorescent
vascular reactivity
calcium
