摘要
研究探讨了脱氧雪腐镰刀菌烯醇(DON)对人外周血单个核细胞HLA-I(human leuco-cyte antigen I)分子表达影响。采用流式细胞术(FCM)和免疫印迹方法研究了不同剂量DON对体外培养人外周血单个核细胞表面HLA-I分子表达的影响及其量效关系。FCM定量检测结果表明,不同浓度DON处理均可一定程度降低人外周血单个核细胞表面HLA-I分子的表达,DON 50ng/mL、100 ng/mL、1000 ng/mL和2000 ng/mL组HLA-I类分子的平均表达量分别为6.92±0.68、6.64±0.69、5.95±0.48和5.48±0.77,在50～2000ng/mL范围内随着DON浓度增加,外周血单个核细胞HLA-I分子表达降低,两者是显著负相关(r=0.737,P<0.01)。Western印迹结果显示,大剂量DON(1000ng/mL和2000 ng/mL)组人外周血单个核细胞HLA-I分子表达明显减弱。研究结果表明脱氧雪腐镰刀菌烯醇可剂量依赖地抑制体外培养的人外周血单个核细胞HLA-I分子的表达。
To explore the effects of deoxynivalenol on HLA-Ⅰ expression of human peripheral blood
mononuclear cells. Effects of deoxynivalenol at different concentration on HLA-Ⅰ expression of human peripheral
blood mononuclear cells in vitro were studied with flow cytometry(FCM) analysis and Western blotting. FCM
analysis indicated that HLA-Ⅰ expressions of human peripheral blood mononuclear cells were reduced in DON
treatment groups (50ng/mL, 100ng/mL, 1000ng/mL and 2000ng/mL), especially in relatively higher concentration
DON (1000ng/mL and 2000ng/mL) groups (P<0.01), compared to that in control. In the concentration range from
50ng/mL to 2000ng/mL, a significant concentration-depended negative correlation could be found between HLA-
Ⅰ expression and DON concentration. Western blotting also confirmed that DON at higher concentration (1000ng/
mL and 2000ng/mL) could significantly inhibit HLA-Ⅰ expression at protein level. The results suggest that DON
could reduce HLA-Ⅰ expression of human peripheral blood mononuclear cells in concentration-dependent manner
in vitro.
出处
《细胞生物学杂志》
CSCD
北大核心
2004年第3期297-300,共4页
Chinese Journal of Cell Biology
基金
国家科技部基础研究重大项目前期研究专项资助项目(批准号:2001CCC00500)
河北省自然科学基金(批准号:301350)
