摘要
目的 :以大鼠骨髓基质细胞为种子细胞 ,胶原 -壳聚糖复合物为支架材料 ,通过骨组织工程的原理和方法修复牙槽骨缺损。方法 :将 2 8只SD大鼠分为实验组和对照组 ,实验组取大鼠骨髓基质细胞进行体外培养并矿化诱导为成骨样细胞 ,植入胶原 -壳聚糖复合物培养 1周后 ,植入大鼠下颌骨缺损处 ,2周、4周后取材。对照组细胞未进行诱导。结果 :骨髓基质细胞矿化诱导后表现出与成骨细胞相似的形态学与功能表现 ,ALP染色阳性 ,并形成矿化结节 ,细胞在胶原 -壳聚糖复合物内伸展良好 ,组织学观察 :实验组 2周后可见有少量新骨形成 ,4周后新骨形成面积增大对照组无明显新骨形成 ,材料周围有炎细胞浸润。并可见部分纤维结缔组织包绕。结论 :利用胶原 -壳聚糖复合物与诱导后的BMSC复合有良好的新骨形成能力。
AIM:We use SD rats bone marrow stromal cells (BMSC) as seed cells,the composite of collagen and chitosan as scaffold,to repair the loss of SD rats alveolar.METHODS:28 SD rats were divided into experimental group,and the control group.The BMSC of experimental group were cultivated and induced to be osteoblast like cells,and embedded into the composite of chitosan and collagen one weer later,embedded the new composite to the defect of SD rats alveolar.Two weeks and four weeks observed the results.In the control group,the BMSC were not indueed.RESULTS:BMSC had the similar morphology and function with osteoblast after cultivation,mineralization and induction.ALP dying positive,and there was mineralization nodus formation.In the experimental group,two weeks later,certain amount of new bone formation could be seen.Four weeks later,the area of new bone formation increased.While in the control groups,no bone formation was observed,some inflammatory cells,and some fibro.connective tissues appeared.CONCLUSION:The composite of collagen and chitosan combines with induced BMSC can reproduce new bone.It has the potential in clinical repairing the bone defect of clefts and making their orthodontic treatment easier.
出处
《牙体牙髓牙周病学杂志》
CAS
2004年第5期246-249,共4页
Chinese Journal of Conservative Dentistry
关键词
骨髓基质细胞
胶原-壳聚糖复合物
组织工程化骨
bone marrow stromal cells BMSC
chotisan and collegen composite
tissue engineering bone
