摘要
目的:通过建立高温高湿环境下大鼠腹部创伤动物模型,用局部氧气干预创面的方法,探讨这一特殊环境下创面组织的病理形态学变化以及局部氧气干预对创面愈合的影响,为临床伤口护理提供理论依据。方法:将24只创伤大鼠随机分为常温对照组(NEC)、常温氧疗组(NEO)、高温对照组(HHE)、高温氧疗组(HHO)4组,每组6只。在相应干预后的不同时间对各组动物的平均动脉压(MAP)、创面愈合时间、细菌学定量、病理形态学进行观察和检测。结果:HHE组较NEC组创面肿胀程度严重,创面愈合时间长,HHO组较HHE组创面肿胀程度轻,创面愈合时间短。光镜和电镜观察到HHE组创面肌肉纤维的病理变化均较NEC组显著,且损伤呈进行性加重,HHO组肌肉组织损伤程度较HHE组轻。细菌学定量检测HHE组创面细菌数在同一时相比NEC组高,且随时间的延长呈显著增长趋势,HHO组创面细菌数量与HHE组比较差异有显著性意义(P<0.05)。结论:高温高湿环境下创面组织病理损伤严重,细菌繁殖快,感染时间提前且严重,创面愈合速度慢;局部氧气干预可抑制创面组织细菌繁殖,对大鼠腹部刀割创面有明显的促修复作用。
AIM:To establish belly wound animal model of rats under high temperature and high moisture so as to evaluate the effect of oxygen therapy intervention on wound healing and pathomorphology of the wound organization under the special environment in order to supply theory foundation for the clinical wound nursing. METHOD:Twenty four injured rats were randomly divided into four groups, including normal environment control group(NEC),normal environment oxygen therapy group(NEO),hot and humid environment control group (HHE),hot and humid environment oxygen therapy group (HHO).Mean arterial pressure(MAP), wound healing time, bacteriology ration and pathomorphology of different groups were observed and detected during different periods after the corresponding intervention. RESULTS:The swollen degree of wound surface in HHE group was more serious and the wound healing time was longer as compared with those in NEC group.The swollen degree of wound surface in HHO group was less serious and the wound healing time was shorter as compared with those in HHE group. Under light microscope and electron microscope,the pathology changes of the muscle fibers in HHE group were more significant than those in NEC group, and the damages were aggravated. The damage degree of the muscular tissue in HHO group was better than that in HHE group.Bacterial quantification at the wound showed that the number of bacteria in HHE group was higher than that in NEC group at the same time, and the difference tended to increase with the extending of time. Bacterial quantification in HHO group was significant different from that in HHE group(P< 0.05). CONCLUSION:In hot and humid environment, pathology damage of wound organization is serious, bacteria proliferation is accelerated to result in earlier and more serious occurrence of infection and the wound healing time is prolonged.Local oxygen intervention can restrain the bacterial proliferation,which has significant effect in repairing belly wound of rsts.
出处
《中国临床康复》
CSCD
2004年第17期3303-3305,共3页
Chinese Journal of Clinical Rehabilitation
基金
全军医学科研基金"十五"计划资助(01MAI33)~~
