摘要
采用聚丙烯酰胺凝胶垂直平板电泳方法 ,研究分析了口虾蛄 (Oratosquillaoratoria)肌肉、眼球、腹鳃、心脏和肝胰腺 5种器官或组织中LDH、ADH、MDH、SDH、MEP、POD、AMY、GDH、SCD、EST、GLDH和SOD共 1 2种同工酶的表达情况。结果表明 ,口虾蛄同工酶的表达具有明显的组织特异性。对 1 2种同工酶进行生化遗传分析 ,获得了基本酶谱。 1 2种同工酶共记录出35个基因座位 ,其中 1 1个基因座位Ldh 3、Adh 1、Sdh 2、Est 1、Est 2、Est 3、Mdh、Mep 7、Gldh 1、Gldh 2和Scd 1为多态 ,其多态座位比例为 31 43%。
Oratosquilla oratoria is an economically valuable marine crustacean in northeast Asia, such as China, Japan and Korea. Prompted by the absence of any existing isozyme studies on O. oratoria , this paper studied the genetic expression of isozyme of O. oratoria . Thirty six live O. oratoria were collected from Yuehu market of Ningbo, China during 2001 and 2002. In total, one hundred and eighty biochemical samples from five tissues and organs (eye, muscle, ventral gill, heart and hepatopancreas) were taken and, either stored immediately in ultra low temperature freezer, or tested directly in situ. The samples were washed with double distilled water and blotted with disinfected filter paper before storage or testing. Twelve isozymes (LDH, MDH, ADH, MEP, SDH, SCD, SOD, GDH, GLDH, AMY, EST, POD) in a buffer system of TG (Tris Gly, pH8.3) were tested using vertical polyacrylamide gel electrophoresis (PAGE). Expression in 5 tissues and organ tissues was also tested. Results showed that isozymes expression contained clear tissue specificity, indicating that variations between different tissues of O. oratoria was a common phenomenon. The basic function of muscle is to move; therefore LDH showed a strong activity in muscle. The function of ventral gills is to respire, MDH making causing weak LDH's activity weak in ventral gills is much more strongly expressed in muscle and heart than in other tissues or organs, similar results to those for Penaeus chinensis . Biochemical genetics were analyzed in terms of their locus number, structure, alleles, etc. On the basis of these results, basic electrophoretic patterns of these twelve isozymes were obtained. Altogether, 35 gene loci were recorded, among which 11 gene loci included Ldh _3 (alleles, 100, 110), Adh _1 (alleles, 100, 105), Sdh _2 (alleles, 95, 100, 105), Est _1 (alleles, 100, 110), Est _2 (alleles, 100, 110), Est _3 (alleles, 100, 105), Mdh (alleles, 90, 100), Mep _7 (alleles, 75, 100), Gldh _1 (alleles, 100, 110), Gldh _2 (alleles, 100,110) and Scd _1 (alleles, 100, 115) were polymorphic. In addition, the mean proportion of polymorphic loci was 31.43%, higher than that of P. chinensis (15% or 20%). Results of this study suggest that the current variation state of O. oratoria is good. We must protect well this valuable resource from being damaged.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2004年第3期258-263,共6页
Oceanologia Et Limnologia Sinica
基金
国家农业科技成果转化资金项目
0 2EFN2 1 331 0 6 5 1号
浙江省自然科学基金资助项目
30 2 4 4 2号
关键词
口虾蛄
同工酶
组织特异性
生化遗传分析
Oratosquilla oratoria , Isozyme, Tissue specificity, Biochemical genetic analysis
