摘要
目的 探讨反义端粒酶肽核酸 (PNA)片段对肺癌细胞株端粒酶活性及细胞株生长的抑制作用。方法 将人工合成的端粒酶反义PNA片段 ,应用脂质体转染法将反义端粒酶序列导入肺腺癌细胞株A5 49及小细胞癌细胞株NCI H44 6中 ,采用MTT法检测活细胞数 ,采用RT PCR ELLISA法检测端粒酶活性。结果 转染 72h后 ,A5 49、NCI H 44 6细胞株端粒酶活性 (A45 0值 )分别由 0 .5 82± 0 .0 3 9和 0 .5 71± 0 .0 43降低至 0 .2 94± 0 .0 48(P <0 .0 1)和 0 .2 76± 0 .0 5 1(P <0 .0 1) ;而两细胞株的活细胞数 (A5 80值 )分别由 0 .485± 0 .0 0 9和 0 .5 13± 0 .0 15降低至 0 .191± 0 .0 2 7(P <0 .0 1)和 0 .13 8± 0 .0 46(P <0 .0 1) ,细胞生长受到显著抑制。结论 反义端粒酶PNA片段具有抑制肺癌细胞株端粒酶活性的作用 。
Objective To determine the inhibitory effect of antisense peptide nucleic acids (PNA) of telomerase on the growth of lung cancer cell lines. Methods The synthesized modified antisense PNAs of telomerase were transfected into the lung cancer cell lines A549 and NCI H446 respectively by lipofectamine transfection. Telomerase activity was detected by RT PCR ELLISA, and the cell counts were determined by MTT. Results Seventy two hours after transfection with antisense PNAs of telomerase, telomerase activity (A450 value) of A549 and NCI H446 were down regulated from 0.582 ±0.039, 0.571±0.043 to 0.294±0.048 ( P <0.01), 0.276±0.051 ( P <0.01) respectively, and alive cell counts (A580 value) of them from 0.485± 0.009 , 0.513±0.015 to 0.191±0.027 ( P <0.01), 0.138±0.046 ( P <0.01) respectively. The growth of two lung cancer cell lines were significantly inhibited. Conclusion Antisense PNA of telomerase might inhibit not only the telomerase activity, but also the growth of lung cancer cell lines in vitro .
出处
《中国肺癌杂志》
CAS
2004年第3期196-198,共3页
Chinese Journal of Lung Cancer
关键词
肺肿瘤
反义
端粒酶
肽核酸
Lung neoplasms Antisense Telomerase Peptide nucleic acids
