摘要
目的 通过生物转化对去氢表雄酮进行结构修饰 ,以期获得更有意义的转化产物。方法 利用唐古特山莨菪毛状根液体悬浮培养体系对去氢表雄酮进行生物转化 ,通过柱色谱及制备薄层色谱进行分离纯化 ,利用核磁共振、ESI MS等光谱鉴定结构。结果 分离得到了 5个转化产物 ,即 :androst 4 ene 3,1 7 dione (I) ;6α hydroxyandrost 4 ene 3,1 7 dione (II) ;6α ,1 7β dihydroxyandrost 4 ene 3 one(III) ;androst 4 ene 3,6 ,1 7 trione (IV) ;1 7β hydroxyandrost 4 ene 3 one (V)。结论 所得到的
Aim To modify the structure of dehydroepiandrosterone (DHEA) . Methods Using hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products. Results (1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst 4 ene 3,17 dione (I); 6α hydroxyandrost 4 ene 3 ,17 dione (II) ; 6α,17β dihydroxyandrost 4 ene 3 one (III); androst 4 ene 3,6,17 trione (IV) and 17β hydroxyandrost 4 ene 3 one (V). Conclusion It is the first time to use hairy root cultures of Anisodus tanguticus for the biotransformation of DHEA and five DHEA related compounds were obtained.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第6期445-448,共4页
Acta Pharmaceutica Sinica
基金
国家 8 63项目资助 ( 2 0 0 2AA2Z3 43B)
